2024
Antiphospholipid-exposed trophoblast-derived extracellular vesicles express elevated levels of TLR7/8-activating microRNAs and induce endometrial endothelial activation, in part, through TLR7
Coenen C, Hidalgo T, Lynn T, Jones D, Salmon J, Chamley L, Abrahams V. Antiphospholipid-exposed trophoblast-derived extracellular vesicles express elevated levels of TLR7/8-activating microRNAs and induce endometrial endothelial activation, in part, through TLR7. Journal Of Reproductive Immunology 2024, 164: 104255. PMID: 38797133, DOI: 10.1016/j.jri.2024.104255.Peer-Reviewed Original ResearchHuman endometrial endothelial cellsObstetric antiphospholipid syndromeToll-like receptor 4MiR-146a-3pMaternal-fetal interfaceAntiphospholipid syndromeIL-8 responseEndometrial endothelial cellsTrophoblast cellsExtracellular vesiclesElevated levels of IL-8IL-8Endothelial cellsLevels of IL-8Activation of Toll-like receptor 4Downstream of toll-like receptor 4Exposure to aPLElevated levelsExtravillous trophoblast cellsHuman trophoblast cellsInsufficient placentationTrophoblast-derived extracellular vesiclesAntiphospholipid antibodiesMaternal vasculaturePlacental trophoblastsTLR8‐activating miR‐146a‐3p is an intermediate signal contributing to fetal membrane inflammation in response to bacterial LPS
Georges H, Cassin C, Tong M, Abrahams V. TLR8‐activating miR‐146a‐3p is an intermediate signal contributing to fetal membrane inflammation in response to bacterial LPS. Immunology 2024, 172: 577-587. PMID: 38631842, PMCID: PMC11223956, DOI: 10.1111/imm.13794.Peer-Reviewed Original ResearchMiR-146a-3pPreterm birthFetal membranesToll-like receptorsExpression of miR-146a-3pLipopolysaccharide exposureMouse model of pregnancyIL-8Response to bacterial LPSAssociated with chorioamnionitisFetal membrane inflammationHuman FM explantsMouse fetal membranesInduced preterm birthBacterial lipopolysaccharideMaternal-fetal interfaceDanger signalsWild-type miceIn vivo mouse modelModel of pregnancyInterleukin (IL)-8Pro-inflammatory roleResponse to bacterial lipopolysaccharideAssociated with elevated levelsResponse to LPS
2018
Role of NOD2 in antiphospholipid antibody-induced and bacterial MDP amplification of trophoblast inflammation
Mulla MJ, Pasternak MC, Salmon JE, Chamley LW, Abrahams VM. Role of NOD2 in antiphospholipid antibody-induced and bacterial MDP amplification of trophoblast inflammation. Journal Of Autoimmunity 2018, 98: 103-112. PMID: 30594350, DOI: 10.1016/j.jaut.2018.12.003.Peer-Reviewed Original ResearchConceptsMuramyl dipeptideAbsence of aPLAnti-angiogenic profileNovel danger signalPresence of aPLRole of NOD2IL-1β expressionMaternal-fetal interfaceActivation of NOD2IL-1β productionBacterial muramyl dipeptideAntiphospholipid antibodiesCommon bacterial componentsPregnancy complicationsPregnancy outcomesAdverse outcomesIL-1βTrophoblast responsesExtravillous trophoblastsHigh riskPreeclampsiaDanger signalsBacterial infectionsVEGF secretionInflammation
2017
Mechanisms of Antiphospholipid Antibody-Mediated Pregnancy Morbidity
Salmon J, Mineo C, Giles I, Chamley L, Meroni P, Abrahams V. Mechanisms of Antiphospholipid Antibody-Mediated Pregnancy Morbidity. 2017, 117-143. DOI: 10.1007/978-3-319-55442-6_6.Peer-Reviewed Original ResearchIntrauterine growth restrictionPregnancy complicationsAntiphospholipid syndromeAntiphospholipid antibodiesRecurrent early pregnancy lossImpaired placental functionLate obstetrical complicationsNon-thrombotic eventsPartial beneficial effectEarly pregnancy lossMaternal-fetal interfacePregnancy morbidityAPS patientsObstetrical complicationsThrombotic eventsTrophoblast functionsPregnancy lossGrowth restrictionPlacental functionHigh riskComplicationsPathogenic mechanismsComplement activationBeneficial effectsPatients
2014
Single- and double-stranded viral RNA generate distinct cytokine and antiviral responses in human fetal membranes
Bakaysa SL, Potter JA, Hoang M, Han CS, Guller S, Norwitz ER, Abrahams VM. Single- and double-stranded viral RNA generate distinct cytokine and antiviral responses in human fetal membranes. Molecular Human Reproduction 2014, 20: 701-708. PMID: 24723465, PMCID: PMC4072183, DOI: 10.1093/molehr/gau028.Peer-Reviewed Original ResearchConceptsHuman fetal membranesToll-like receptorsMIP-1βMIP-1αFetal membranesAntiviral responseIL-1βIL-6IL-2Viral infectionG-CSFCytokine/chemokine profilesViral ssRNAAdverse pregnancy outcomesMaternal-fetal interfaceInfection-associated inflammationMCP-1 secretionViral dsRNA analogueTNF-α productionMIP-1α secretionTLR adapter proteinsExpression of IFNβPregnancy outcomesCytokine profileChemokine profilesHuman Fetal Membranes Generate Distinct Cytokine Profiles in Response to Bacterial Toll-Like Receptor and Nod-Like Receptor Agonists1
Hoang M, Potter JA, Gysler SM, Han CS, Guller S, Norwitz ER, Abrahams VM. Human Fetal Membranes Generate Distinct Cytokine Profiles in Response to Bacterial Toll-Like Receptor and Nod-Like Receptor Agonists1. Biology Of Reproduction 2014, 90: 39, 1-9. PMID: 24429216, PMCID: PMC4076407, DOI: 10.1095/biolreprod.113.115428.Peer-Reviewed Original ResearchConceptsBacterial toll-like receptorsToll-like receptorsPathogen-associated molecular patternsInnate immune pattern recognition receptorsNOD-like receptorsPattern recognition receptorsImmune pattern recognition receptorsCytokine profileIL1B secretionFetal membranesCpG ODNCaspase-1Subsequent preterm birthInflammatory cytokine profilePreterm premature ruptureDistinct cytokine profilesMaternal-fetal interfaceInfection-associated inflammationMild stimulatory effectNLR agonistsPreterm birthPremature ruptureProinflammatory cytokinesCytokine productionMCP-1
2013
A Role for Uric Acid and the Nalp3 Inflammasome in Antiphospholipid Antibody-Induced IL-1β Production by Human First Trimester Trophoblast
Mulla MJ, Salmon JE, Chamley LW, Brosens JJ, Boeras CM, Kavathas PB, Abrahams VM. A Role for Uric Acid and the Nalp3 Inflammasome in Antiphospholipid Antibody-Induced IL-1β Production by Human First Trimester Trophoblast. PLOS ONE 2013, 8: e65237. PMID: 23762324, PMCID: PMC3675211, DOI: 10.1371/journal.pone.0065237.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, AntiphospholipidAntiphospholipid SyndromeBeta 2-Glycoprotein ICARD Signaling Adaptor ProteinsCarrier ProteinsCaspase 1Cell LineCytoskeletal ProteinsFemaleGene Expression RegulationHumansInflammasomesInterleukin-1betaMiceNLR Family, Pyrin Domain-Containing 3 ProteinPregnancyPregnancy Trimester, FirstProtein PrecursorsSignal TransductionToll-Like Receptor 4TrophoblastsUric AcidConceptsIL-1β productionIntrauterine growth restrictionIL-1β secretionAntiphospholipid syndromeIL-1β processingAntiphospholipid antibodiesIL-1βHuman first trimester trophoblast cell lineFirst trimester trophoblast cell lineUric acidNalp3/ASC inflammasomeHuman first trimester trophoblast cellsToll-like receptor 4Human first-trimester trophoblastsFirst trimester trophoblast cellsAnti-β2GPI AbsAdverse pregnancy outcomesAnti-β2GPI antibodiesRecurrent pregnancy lossTLR4-dependent mannerApoptosis-associated speck-like proteinMaternal-fetal interfaceFirst trimester trophoblastsSpeck-like proteinTrophoblast cell line
2012
What is the Mechanism(s) of Antiphospholipid Antibody-Mediated Pregnancy Morbidity?
Abrahams V, Borghi M, Meroni P, Rand J, Raschi E, Salmon J, Tedesco F, Tincani A. What is the Mechanism(s) of Antiphospholipid Antibody-Mediated Pregnancy Morbidity? 2012, 79-101. DOI: 10.1007/978-1-4614-3194-7_5.Peer-Reviewed Original ResearchPregnancy complicationsAntiphospholipid syndromeAntiphospholipid antibodiesRecurrent early pregnancy lossImpaired placental functionLate obstetrical complicationsNon-thrombotic eventsPartial beneficial effectIntrauterine growth restrictionEarly pregnancy lossMaternal-fetal interfacePregnancy morbidityAPS patientsObstetrical complicationsThrombotic eventsTrophoblast functionsPregnancy lossGrowth restrictionPlacental functionHigh riskComplicationsPathogenic mechanismsComplement activationBeneficial effectsPatients
2011
Nod1 Activation by Bacterial iE-DAP Induces Maternal–Fetal Inflammation and Preterm Labor
Cardenas I, Mulla MJ, Myrtolli K, Sfakianaki AK, Norwitz ER, Tadesse S, Guller S, Abrahams VM. Nod1 Activation by Bacterial iE-DAP Induces Maternal–Fetal Inflammation and Preterm Labor. The Journal Of Immunology 2011, 187: 980-986. PMID: 21677137, DOI: 10.4049/jimmunol.1100578.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornCell LineDiaminopimelic AcidDisease Models, AnimalFemaleHumansInfant, NewbornInfant, PrematureInflammationMaternal-Fetal ExchangeMiceMice, Inbred C57BLNod1 Signaling Adaptor ProteinObstetric Labor, PrematurePregnancyPregnancy OutcomeTissue Culture TechniquesTrophoblastsConceptsPattern recognition receptorsPreterm laborCytokine profileNOD1 activationRecognition receptorsInfection-associated preterm laborHuman first-trimester trophoblastsIntracellular pattern recognition receptorsProinflammatory cytokine profileHuman term placental tissueActivation of NOD1Maternal-fetal interfaceInfection-associated inflammationFunction of NOD1Term placental tissueFirst trimester trophoblastsThird trimester trophoblastFetal inflammationPreterm deliveryEmbryonic day 14.5Pregnancy outcomesFetal weightIL-6MCP-1NOD2 stimulation
2006
Trophoblast Cells as Immune Regulators
Mor G, Abrahams V. Trophoblast Cells as Immune Regulators. Medical Intelligence Unit 2006, 215-228. PMCID: PMC7120375, DOI: 10.1007/0-387-34944-8_19.Peer-Reviewed Original ResearchToll-Like Receptors and Pregnancy
Abrahams V, Mor G. Toll-Like Receptors and Pregnancy. Medical Intelligence Unit 2006, 15-25. DOI: 10.1007/0-387-34944-8_2.Peer-Reviewed Original ResearchMaternal-fetal interfaceToll-like receptorsCertain pregnancy complicationsInnate immune cellsInnate immune responseInnate immune systemAllogenic fetusIntrauterine infectionPregnancy complicationsTrophoblast responsesImmune cellsClinical studiesImmune responseImmune systemTrophoblast cellsHost defensePossible pathogensPregnancyStrong associationReceptorsCellsComplicationsPathogensTLRFetuses