2021
The zinc-finger protein OEF-1 stabilizes histone modification patterns and promotes efficient splicing in the Caenorhabditis elegans germline
McManus CE, Mazzetto M, Wei G, Han M, Reinke V. The zinc-finger protein OEF-1 stabilizes histone modification patterns and promotes efficient splicing in the Caenorhabditis elegans germline. G3: Genes, Genomes, Genetics 2021, 11: jkab329. PMID: 34519784, PMCID: PMC8664474, DOI: 10.1093/g3journal/jkab329.Peer-Reviewed Original ResearchConceptsH3K36me3 levelsAutosomal lociC. elegans germ lineGerm cellsC. elegans germlineActive chromatin stateHistone modification patternsMES-4Chromatin stateEpigenetic balanceMutant phenotypeCaenorhabditis elegansSex chromosomesGenomic domainsGerm lineEfficient splicingDifferentiation programX chromosomeGenetic informationModification patternsTranscript integrityEfficient mRNANovel modifierH3K36me3Downstream effects
2019
Isolated C. elegans germ nuclei exhibit distinct genomic profiles of histone modification and gene expression
Han M, Wei G, McManus CE, Hillier LW, Reinke V. Isolated C. elegans germ nuclei exhibit distinct genomic profiles of histone modification and gene expression. BMC Genomics 2019, 20: 500. PMID: 31208332, PMCID: PMC6580472, DOI: 10.1186/s12864-019-5893-9.Peer-Reviewed Original ResearchConceptsHistone modificationsGerm cellsC. elegansC. elegans germ cellsGene expressionDevelopmental gene expressionGene expression mechanismsIsolation of nucleiGene regulationTranscriptome analysisChromatin immunoprecipitationGerm lineGenomic analysisDistinct genomic profilesRegulatory mechanismsRepressive mechanismsExpression mechanismElegansPure populationsGenomic profilesConsequent discoveriesCellsExpressionGenomeNucleus
2018
The Germline-Specific Factor OEF-1 Facilitates Coordinated Progression Through Germ Cell Development in Caenorhabditis elegans
McManus CE, Reinke V. The Germline-Specific Factor OEF-1 Facilitates Coordinated Progression Through Germ Cell Development in Caenorhabditis elegans. Genetics 2018, 208: 549-563. PMID: 29167199, PMCID: PMC5788521, DOI: 10.1534/genetics.117.1123.Peer-Reviewed Original ResearchConceptsGerm cell developmentGerm cellsCell developmentGermline-expressed genesNovel nuclear proteinCell cycle checkpointsGermline expressionGenomic integrityUnique fateOocyte differentiationFaithful transmissionCycle checkpointsNuclear proteinsMature gametesX chromosomeDevelopmental eventsLarval stagesMaturation eventsGenetic materialCaenorhabditisFactor 1Developmental progressionSubtle defectsCellsExpression
2013
Tissue-specific direct targets of Caenorhabditis elegans Rb/E2F dictate distinct somatic and germline programs
Kudron M, Niu W, Lu Z, Wang G, Gerstein M, Snyder M, Reinke V. Tissue-specific direct targets of Caenorhabditis elegans Rb/E2F dictate distinct somatic and germline programs. Genome Biology 2013, 14: r5. PMID: 23347407, PMCID: PMC4053757, DOI: 10.1186/gb-2013-14-1-r5.Peer-Reviewed Original ResearchConceptsRb/E2FLin-35Target genesGenome-wide binding profilesGene expressionTissue-specific gene regulationLin-35 mutantsDistinct cell fatesSmall RNA pathwaysEffector target genesDirect target geneBinding profileGermline programHPL-2Chromatin associationH3K36 methylationRNA pathwaysCSR-1Germline transformationC. elegansGene regulationCell fateE2FDirect targetMultiple tissues
2011
C. elegans meg‐1 and meg‐2 differentially interact with nanos family members to either promote or inhibit germ cell proliferation and survival
Kapelle WS, Reinke V. C. elegans meg‐1 and meg‐2 differentially interact with nanos family members to either promote or inhibit germ cell proliferation and survival. Genesis 2011, 49: 380-391. PMID: 21305687, DOI: 10.1002/dvg.20726.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCaenorhabditis elegansCaenorhabditis elegans ProteinsCell ProliferationCell SurvivalDisorders of Sex DevelopmentEmbryo, NonmammalianEpistasis, GeneticFemaleGene Expression Regulation, DevelopmentalGerm CellsLuminescent ProteinsMaleRNA InterferenceRNA-Binding ProteinsTime FactorsConceptsGerm cell proliferationMeg 1Germ cellsEmbryonic primordial germ cellsTargeted RNAi screenPrimordial germ cellsCell proliferationGerm cell survivalGerm lineageP granulesRNAi screenLarval stagesMEG 2Cell survivalFamily membersMultiple pathwaysGerm cell degenerationPhenotypeSterilityProliferationOptimal proliferationCellsCell degenerationEmbryogenesisLineages
2010
Genome-wide analysis of germ cell proliferation in C . elegans identifies VRK-1 as a key regulator of CEP-1/p53
Waters K, Yang AZ, Reinke V. Genome-wide analysis of germ cell proliferation in C . elegans identifies VRK-1 as a key regulator of CEP-1/p53. Developmental Biology 2010, 344: 1011-1025. PMID: 20599896, PMCID: PMC3375680, DOI: 10.1016/j.ydbio.2010.06.022.Peer-Reviewed Original ResearchConceptsGerm cell proliferationVRK-1Germ cellsCEP-1CEP-1/p53Cell proliferationImportant regulatory relationshipsGenome-wide analysisGene expression profilingGermline proliferationCell cycle arrestUseful model systemC. elegansProliferation defectFunctional characterizationNegative regulationExpression profilingRegulatory relationshipsKey regulatorP53 activityCycle arrestUnsuspected mechanismElegansModel systemProliferation
2009
DPL-1 (DP) acts in the germ line to coordinate ovulation and fertilization in C. elegans
Chi W, Reinke V. DPL-1 (DP) acts in the germ line to coordinate ovulation and fertilization in C. elegans. Cells And Development 2009, 126: 406-416. PMID: 19368797, PMCID: PMC2680456, DOI: 10.1016/j.mod.2009.01.008.Peer-Reviewed Original ResearchConceptsEFL-1/DPLDpl-1Germ lineTissue-specific rescue experimentsGonad armsItr-1RME-2Caenorhabditis elegansEfl-1C. elegansGene functionTranscriptional programsInositol triphosphate receptorGonad structureSuccessful reproductionSevere defectsRescue experimentsGerm cellsFunction mutationsSuccessful fertilizationLikely disruptsMutantsSpermathecaTriphosphate receptorElegans
2008
MEG-1 and MEG-2 Are Embryo-Specific P-Granule Components Required for Germline Development in Caenorhabditis elegans
Leacock SW, Reinke V. MEG-1 and MEG-2 Are Embryo-Specific P-Granule Components Required for Germline Development in Caenorhabditis elegans. Genetics 2008, 178: 295-306. PMID: 18202375, PMCID: PMC2206079, DOI: 10.1534/genetics.107.080218.Peer-Reviewed Original ResearchConceptsP granulesMeg 1Germline developmentGerm lineageMEG 2Embryonic germ lineagesP-granule componentEmbryonic cell divisionGerm cell proliferationGLH-1Germ granulesSomatic lineagesCaenorhabditis elegansGermline segregationMis-segregationMaternal germlineCell divisionGerm cellsPhenotype increasesLineagesGermline defectsPGL-1MutantsProliferationGranules
2006
Regulation of developmental rate and germ cell proliferation in Caenorhabditis elegans by the p53 gene network
Derry W, Bierings R, van Iersel M, Satkunendran T, Reinke V, Rothman J. Regulation of developmental rate and germ cell proliferation in Caenorhabditis elegans by the p53 gene network. Cell Death & Differentiation 2006, 14: 662-670. PMID: 17186023, DOI: 10.1038/sj.cdd.4402075.Peer-Reviewed Original ResearchConceptsCEP-1Genotoxic stressP53 family membersComplex transcriptional regulatory networksDevelopmental rateTranscriptional regulatory networksCell proliferationP53-binding siteGerm cell proliferationTumor suppressor p53Absence of stressGermline apoptosisCaenorhabditis elegansTranscriptional networksC. elegansMammalian counterpartsCheckpoint responseGene networksRegulatory networksTranscriptional targetsP53 gene networkEmbryonic viabilityHuman p63Negative regulatorP53 family
2002
A Targeted RNAi Screen for Genes Involved in Chromosome Morphogenesis and Nuclear Organization in the Caenorhabditis elegans Germline
Colaiácovo M, Stanfield G, Reddy K, Reinke V, Kim S, Villeneuve A. A Targeted RNAi Screen for Genes Involved in Chromosome Morphogenesis and Nuclear Organization in the Caenorhabditis elegans Germline. Genetics 2002, 162: 113-128. PMID: 12242227, PMCID: PMC1462232, DOI: 10.1093/genetics/162.1.113.Peer-Reviewed Original ResearchConceptsChromosome morphogenesisNuclear organizationRNA interferenceCaenorhabditis elegans germlineFunctional genomic strategiesMeiotic prophase eventsTargeted RNAi screenGene expression surveyDNA microarray technologyGermline phenotypeGermline proliferationMeiosis genesChromosome organizationMitotic growthRNAi screenGenomic strategiesMeiotic progressionMeiotic prophaseExpression profilesMicroarray technologyGermlineGenesMorphogenesisCaenorhabditisProphaseX-chromosome silencing in the germline of C. elegans.
Kelly WG, Schaner CE, Dernburg AF, Lee MH, Kim SK, Villeneuve AM, Reinke V. X-chromosome silencing in the germline of C. elegans. Development 2002, 129: 479-92. PMID: 11807039, PMCID: PMC4066729, DOI: 10.1242/dev.129.2.479.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditis elegansDisorders of Sex DevelopmentFemaleGene Expression Regulation, DevelopmentalGene SilencingGenes, ReporterGerm CellsGonadsHistonesImmunohistochemistryIn Situ Hybridization, FluorescenceMaleMeiosisMethylationOligonucleotide Array Sequence AnalysisOocytesRecombinant Fusion ProteinsSpermatozoaTranscription, GeneticTransgenesX ChromosomeConceptsX chromosomeHistone modificationsC. elegansEarly meiosisX chromosome silencingNematode C. elegansGermline maintenanceNematode phylumHermaphroditic speciesChromatin organizationSex bodyXO malesTranscriptional competenceSex chromosomesTranscriptional activationTransgene arrayOocyte meiosisMeiosisChromosomesRepressive mechanismsElegansSpeciesHeterochromatinHermaphroditesGenome