2024
The Upstream Sequence Transcription Complex dictates nucleosome positioning and promoter accessibility at piRNA genes in the C. elegans germ line
Paniagua N, Roberts C, Gonzalez L, Monedero-Alonso D, Reinke V. The Upstream Sequence Transcription Complex dictates nucleosome positioning and promoter accessibility at piRNA genes in the C. elegans germ line. PLOS Genetics 2024, 20: e1011345. PMID: 38985845, PMCID: PMC11262695, DOI: 10.1371/journal.pgen.1011345.Peer-Reviewed Original ResearchPiRNA genesPiRNA clustersGenomic domainsC. elegans germ lineUpstream sequence transcription complexSmall RNA pathwaysGermline-specific expressionGlobal genome organizationPiRNA precursorsPiRNA pathwayChromosome IVGenome organizationRNA pathwaysChromatin stateTranscribed genesNucleosome positioningNucleosome densityGenome integrityTranscriptional regulationTranscription complexC. elegansPiRNAsGerm lineISW-1Nucleosomal environment
2023
Characterization of the distribution and dynamics of chromatin states in the C. elegans germ line reveals substantial H3K4me3 remodeling during oogenesis
Mazzetto M, Gonzalez L, Sanchez N, Reinke V. Characterization of the distribution and dynamics of chromatin states in the C. elegans germ line reveals substantial H3K4me3 remodeling during oogenesis. Genome Research 2023, 34: gr.278247.123. PMID: 38164610, PMCID: PMC10903938, DOI: 10.1101/gr.278247.123.Peer-Reviewed Original ResearchEpigenetic regulatory mechanismsGerm lineChromatin stateGerm cellsC. elegans germ lineGene expressionRegulatory mechanismsDevelopmental transitionsActive histone modification H3K4me3Most genomic studiesZygotic genome activationSmall RNA pathwaysSpatio-temporal expression patternsHistone modifications H3K4me3Chromatin accessibility dataGerm cell differentiationOverall transcript abundanceUndifferentiated germ cellsGenome activationRNA pathwaysChromatin landscapeChromatin organizationChromatin structureEssential genesHistone modifications
2013
Tissue-specific direct targets of Caenorhabditis elegans Rb/E2F dictate distinct somatic and germline programs
Kudron M, Niu W, Lu Z, Wang G, Gerstein M, Snyder M, Reinke V. Tissue-specific direct targets of Caenorhabditis elegans Rb/E2F dictate distinct somatic and germline programs. Genome Biology 2013, 14: r5. PMID: 23347407, PMCID: PMC4053757, DOI: 10.1186/gb-2013-14-1-r5.Peer-Reviewed Original ResearchConceptsRb/E2FLin-35Target genesGenome-wide binding profilesGene expressionTissue-specific gene regulationLin-35 mutantsDistinct cell fatesSmall RNA pathwaysEffector target genesDirect target geneBinding profileGermline programHPL-2Chromatin associationH3K36 methylationRNA pathwaysCSR-1Germline transformationC. elegansGene regulationCell fateE2FDirect targetMultiple tissues