Massively parallel sequencing of the polyadenylated transcriptome of C. elegans
Hillier LW, Reinke V, Green P, Hirst M, Marra MA, Waterston RH. Massively parallel sequencing of the polyadenylated transcriptome of C. elegans. Genome Research 2009, 19: 657-666. PMID: 19181841, PMCID: PMC2665784, DOI: 10.1101/gr.088112.108.Peer-Reviewed Original ResearchConceptsTrans-spliced leader sequenceSplice junctionsParallel sequencingProtein-coding genesProtein-coding transcriptsPolyadenylated transcriptomeNoncoding transcriptsPutative genesNematode CaenorhabditisSomatic nucleiIntergenic regionGene setsLeader sequenceX chromosomeUnprecedented depthMost sequencesTranscriptsGenesMRNA levelsSequencingNotable regionNovel statistical approachAnnotationQuantitative natureSequence