2021
The zinc-finger protein OEF-1 stabilizes histone modification patterns and promotes efficient splicing in the Caenorhabditis elegans germline
McManus CE, Mazzetto M, Wei G, Han M, Reinke V. The zinc-finger protein OEF-1 stabilizes histone modification patterns and promotes efficient splicing in the Caenorhabditis elegans germline. G3: Genes, Genomes, Genetics 2021, 11: jkab329. PMID: 34519784, PMCID: PMC8664474, DOI: 10.1093/g3journal/jkab329.Peer-Reviewed Original ResearchConceptsH3K36me3 levelsAutosomal lociC. elegans germ lineGerm cellsC. elegans germlineActive chromatin stateHistone modification patternsMES-4Chromatin stateEpigenetic balanceMutant phenotypeCaenorhabditis elegansSex chromosomesGenomic domainsGerm lineEfficient splicingDifferentiation programX chromosomeGenetic informationModification patternsTranscript integrityEfficient mRNANovel modifierH3K36me3Downstream effects
2016
A novel small molecule that disrupts a key event during the oocyte-to-embryo transition in C. elegans
Weicksel SE, Mahadav A, Moyle M, Cipriani PG, Kudron M, Pincus Z, Bahmanyar S, Abriola L, Merkel J, Gutwein M, Fernandez AG, Piano F, Gunsalus KC, Reinke V. A novel small molecule that disrupts a key event during the oocyte-to-embryo transition in C. elegans. Development 2016, 143: 3540-3548. PMID: 27510972, PMCID: PMC5087616, DOI: 10.1242/dev.140046.Peer-Reviewed Original ResearchConceptsEmbryo transitionProtein traffickingEarly embryonic eventsCandidate target genesComplex cellular eventsSmall-molecule screenCaenorhabditis elegansInviable embryosC. elegansEmbryonic lethalityMore key componentsEarly embryogenesisTranscription factorsEarly embryosEmbryonic eventsTarget genesCellular eventsSpecies specificityEggshell integrityNovel small moleculesElegansRemarkable specificityKey eventsOsmotic sensitivityDiverse aspects
2010
Diverse transcription factor binding features revealed by genome-wide ChIP-seq in C. elegans
Niu W, Lu ZJ, Zhong M, Sarov M, Murray JI, Brdlik CM, Janette J, Chen C, Alves P, Preston E, Slightham C, Jiang L, Hyman AA, Kim SK, Waterston RH, Gerstein M, Snyder M, Reinke V. Diverse transcription factor binding features revealed by genome-wide ChIP-seq in C. elegans. Genome Research 2010, 21: 245-254. PMID: 21177963, PMCID: PMC3032928, DOI: 10.1101/gr.114587.110.Peer-Reviewed Original ResearchConceptsTranscription factorsTarget genesGenome-wide ChIP-seqDevelopmental processesSequence-specific transcription factorsNon-coding RNA genesHigh-throughput DNA sequencingSelect target genesSingle transcription factorDiverse developmental stagesTranscript start siteCandidate gene targetsEgl-5Hox factorsVulval differentiationLin-39Caenorhabditis elegansTranscriptional networksRNA genesModENCODE consortiumChIP-seqChromatin immunoprecipitationDevelopmental programMab-5Regulatory networks
2009
DPL-1 (DP) acts in the germ line to coordinate ovulation and fertilization in C. elegans
Chi W, Reinke V. DPL-1 (DP) acts in the germ line to coordinate ovulation and fertilization in C. elegans. Cells And Development 2009, 126: 406-416. PMID: 19368797, PMCID: PMC2680456, DOI: 10.1016/j.mod.2009.01.008.Peer-Reviewed Original ResearchConceptsEFL-1/DPLDpl-1Germ lineTissue-specific rescue experimentsGonad armsItr-1RME-2Caenorhabditis elegansEfl-1C. elegansGene functionTranscriptional programsInositol triphosphate receptorGonad structureSuccessful reproductionSevere defectsRescue experimentsGerm cellsFunction mutationsSuccessful fertilizationLikely disruptsMutantsSpermathecaTriphosphate receptorElegans
2008
MEG-1 and MEG-2 Are Embryo-Specific P-Granule Components Required for Germline Development in Caenorhabditis elegans
Leacock SW, Reinke V. MEG-1 and MEG-2 Are Embryo-Specific P-Granule Components Required for Germline Development in Caenorhabditis elegans. Genetics 2008, 178: 295-306. PMID: 18202375, PMCID: PMC2206079, DOI: 10.1534/genetics.107.080218.Peer-Reviewed Original ResearchConceptsP granulesMeg 1Germline developmentGerm lineageMEG 2Embryonic germ lineagesP-granule componentEmbryonic cell divisionGerm cell proliferationGLH-1Germ granulesSomatic lineagesCaenorhabditis elegansGermline segregationMis-segregationMaternal germlineCell divisionGerm cellsPhenotype increasesLineagesGermline defectsPGL-1MutantsProliferationGranules
2006
Regulation of developmental rate and germ cell proliferation in Caenorhabditis elegans by the p53 gene network
Derry W, Bierings R, van Iersel M, Satkunendran T, Reinke V, Rothman J. Regulation of developmental rate and germ cell proliferation in Caenorhabditis elegans by the p53 gene network. Cell Death & Differentiation 2006, 14: 662-670. PMID: 17186023, DOI: 10.1038/sj.cdd.4402075.Peer-Reviewed Original ResearchConceptsCEP-1Genotoxic stressP53 family membersComplex transcriptional regulatory networksDevelopmental rateTranscriptional regulatory networksCell proliferationP53-binding siteGerm cell proliferationTumor suppressor p53Absence of stressGermline apoptosisCaenorhabditis elegansTranscriptional networksC. elegansMammalian counterpartsCheckpoint responseGene networksRegulatory networksTranscriptional targetsP53 gene networkEmbryonic viabilityHuman p63Negative regulatorP53 familyPromotion of oogenesis and embryogenesis in the C. elegans gonad by EFL-1/DPL-1 (E2F) does not require LIN-35 (pRB)
Chi W, Reinke V. Promotion of oogenesis and embryogenesis in the C. elegans gonad by EFL-1/DPL-1 (E2F) does not require LIN-35 (pRB). Development 2006, 133: 3147-3157. PMID: 16854972, DOI: 10.1242/dev.02490.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesCaenorhabditis elegansCaenorhabditis elegans ProteinsE2F Transcription FactorsEmbryonic DevelopmentFertilityGene Expression Regulation, DevelopmentalGonadsOligonucleotide Array Sequence AnalysisOogenesisReceptors, LDLRepressor ProteinsRNA-Binding ProteinsTranscription FactorsTranscriptional ActivationConceptsEFL-1/DPLLin-35 mutantsLin-35Dpl-1Efl-1Target genesC. elegansTissue-specific transcriptional programsEctopic cell divisionE2F-binding sitesCell cycle componentsCaenorhabditis elegansTranscriptional programsEarly embryogenesisResponsive genesCell divisionCause defectsRegulatory regionsSomatic tissuesExpression profilingGene expressionReduced broodsOogenesisElegansFunction mutations
2000
Genome-wide analysis of developmental and sex-regulated gene expression profiles in Caenorhabditis elegans
Jiang M, Ryu J, Kiraly M, Duke K, Reinke V, Kim S. Genome-wide analysis of developmental and sex-regulated gene expression profiles in Caenorhabditis elegans. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 98: 218-223. PMID: 11134517, PMCID: PMC14571, DOI: 10.1073/pnas.98.1.218.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsCaenorhabditis elegansCyclinsDisorders of Sex DevelopmentFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalGenes, HelminthGenes, RetinoblastomaGenomeGenomicsMaleOligonucleotide Array Sequence AnalysisProto-Oncogene ProteinsSex CharacteristicsSex DifferentiationSignal TransductionTranscription FactorsWnt ProteinsZebrafish ProteinsConceptsDNA microarraysCaenorhabditis elegansFull-genome DNA microarraysGene expressionSex-specific differentiationGenome-wide analysisGene expression profilesDifferent developmental stagesComplete molecular profileGene expression levelsExpression profilesExpression dataGenesDevelopmental stagesMolecular levelWorm strainsElegansExpression levelsMicroarrayGrowth conditionsRelative levelsMolecular profileExpressionGlobal viewHermaphrodites