2022
Proteomic profiling reveals an association between ALDH and oxidative phosphorylation and DNA damage repair pathways in human colon adenocarcinoma stem cells
Wang Y, Chen Y, Garcia-Milian R, Golla JP, Charkoftaki G, Lam TT, Thompson DC, Vasiliou V. Proteomic profiling reveals an association between ALDH and oxidative phosphorylation and DNA damage repair pathways in human colon adenocarcinoma stem cells. Chemico-Biological Interactions 2022, 368: 110175. PMID: 36162455, PMCID: PMC9891852, DOI: 10.1016/j.cbi.2022.110175.Peer-Reviewed Original ResearchConceptsCancer stem cellsProteomic profilingOxidative phosphorylationLabel-free quantitative proteomic analysisDNA damage repair pathwaysQuantitative proteomic analysisAldehyde dehydrogenase familyColon cancer stem cellsCOLO320DM cellsStem cellsNucleotide excision repairDamage repair pathwaysIngenuity Pathway AnalysisCell populationsProteomic analysisProteomic datasetsDehydrogenase familyMetabolic switchProteomic studiesRepair pathwaysCellular pathwaysALDH enzymatic activityCellular survivalExcision repairALDH activity
2018
Global phosphoproteomic analysis identifies SRMS-regulated secondary signaling intermediates
Goel RK, Meyer M, Paczkowska M, Reimand J, Vizeacoumar F, Vizeacoumar F, Lam TT, Lukong KE. Global phosphoproteomic analysis identifies SRMS-regulated secondary signaling intermediates. Proteome Science 2018, 16: 16. PMID: 30140170, PMCID: PMC6098843, DOI: 10.1186/s12953-018-0143-7.Peer-Reviewed Original ResearchGlobal phosphoproteomic analysisPhosphoproteomic analysisCellular processesSignaling intermediatesTyrosine kinaseNon-receptor tyrosine kinaseCasein kinase 2 alphaDNA repair pathwaysBRK familyPotential kinasesSubstrate proteinsPhosphorylation dynamicsUpregulated phosphositesSrc familyBioinformatics analysisRepair pathwaysBioinfomatic analysisCandidate substratesBiological processesApoptotic pathwayKinaseIndirect regulationDatabase searchingPhosphoproteomeAnalysis of motives