2023
Dual regulation of SLC25A39 by AFG3L2 and iron controls mitochondrial glutathione homeostasis
Shi X, DeCiucis M, Grabinska K, Kanyo J, Liu A, Lam T, Shen H. Dual regulation of SLC25A39 by AFG3L2 and iron controls mitochondrial glutathione homeostasis. Molecular Cell 2023, 84: 802-810.e6. PMID: 38157846, PMCID: PMC10922821, DOI: 10.1016/j.molcel.2023.12.008.Peer-Reviewed Original ResearchProtein quality controlMitochondrial protein quality controlDual regulationMetabolic compartmentalizationIron homeostasisMitochondrial iron-sulfur clustersIron-sulfur clustersMitochondrial transportersProtein regulationMammalian cellsCRISPR knockoutCysteine residuesTransporter regulationLoop 1SLC25A39Glutathione homeostasisMetabolic sensingGlutathione uptakeMature neuronsProtein levelsHomeostasisRegulationAFG3L2Biochemical featuresMitochondrial glutathione levels
2021
Small Extracellular Vesicles Control Dendritic Spine Development through Regulation of HDAC2 Signaling
Zhang L, Lin TV, Yuan Q, Sadoul R, Lam TT, Bordey A. Small Extracellular Vesicles Control Dendritic Spine Development through Regulation of HDAC2 Signaling. Journal Of Neuroscience 2021, 41: 3799-3807. PMID: 33741723, PMCID: PMC8084316, DOI: 10.1523/jneurosci.0766-20.2021.Peer-Reviewed Original ResearchConceptsSmall extracellular vesiclesRegulation of HDAC2Extracellular vesiclesSpine developmentCell-cell signalingTranscriptional programsCortical neuronsSEV releaseTranscriptional decreaseDendritic spinesNeuronal developmentNeuron developmentDendritic spine developmentLines of evidenceHDAC2Paracrine communicationAge-dependent decreaseVesiclesPopulations of neuronsRegulationLC-MS/MSHDAC2 levelsSynaptic targetsExcitatory synapsesSpine growth
2019
Phosphorylation of human placental aromatase CYP19A1.
Ghosh D, Egbuta C, Kanyo J, Lam TT. Phosphorylation of human placental aromatase CYP19A1. Biochemical Journal 2019, 476: 3313-3331. PMID: 31652308, PMCID: PMC7069221, DOI: 10.1042/bcj20190633.Peer-Reviewed Original ResearchConceptsAromatase CYP19A1Protein-level regulationPhosphorylation/dephosphorylationMultiple phosphorylation sitesNon-genomic signaling pathwaysPost-translational modificationsNon-genomic signalingTranscriptional activatorPhosphorylation sitesProline residuesSignaling pathwaysHistidine residuesPhosphorylationLevel regulationAromatase inhibitor resistanceInhibitor resistanceMembrane interfaceRegulationReproductive systemStructural dataAromatase activityActive siteResiduesGenotoxic effectsSynaptic terminals
2006
Interaction of packaging motor with the polymerase complex of dsRNA bacteriophage
Lísal J, Kainov DE, Lam TT, Emmett MR, Wei H, Gottlieb P, Marshall AG, Tuma R. Interaction of packaging motor with the polymerase complex of dsRNA bacteriophage. Virology 2006, 351: 73-79. PMID: 16643976, DOI: 10.1016/j.virol.2006.03.025.Peer-Reviewed Original ResearchConceptsC-terminal faceP4 hexamersDsRNA bacteriophagesPackaging motorPolymerase complexHydrogen-deuterium exchangeSubunit interfaceProcapsidRNA loadingBiochemical studiesMolecular motorsEmpty capsidsHexamerViral capsidCapsidMass spectrometryGenomeDsRNAProcessivityBacteriophagesInteractionRegulationAssociates