2023
Proteome-wide screening for mitogen-activated protein kinase docking motifs and interactors
Shi G, Song C, Torres Robles J, Salichos L, Lou H, Lam T, Gerstein M, Turk B. Proteome-wide screening for mitogen-activated protein kinase docking motifs and interactors. Science Signaling 2023, 16: eabm5518. PMID: 36626580, PMCID: PMC9995140, DOI: 10.1126/scisignal.abm5518.Peer-Reviewed Original ResearchConceptsMitogen-activated protein kinaseDocking motifSequence motifsDocking sequenceShort linear sequence motifsLinear sequence motifsSubstrate recruitmentHuman proteomeProtein kinaseCatalytic cleftExchange mutantsEssential functionsCultured cellsScreening pipelineWide screeningInteractorsMotifSequenceLimited repertoireSelective bindingInteractomeCombinatorial librariesMKK6ProteomeMKK7
2021
Phosphoproteomic analysis of lung tissue from patients with pulmonary arterial hypertension
Sitapara R, Lam TT, Gandjeva A, Tuder RM, Zisman LS. Phosphoproteomic analysis of lung tissue from patients with pulmonary arterial hypertension. Pulmonary Circulation 2021, 11: 20458940211031109. PMID: 34966541, PMCID: PMC8711668, DOI: 10.1177/20458940211031109.Peer-Reviewed Original ResearchCyclin-dependent kinase 1Pulmonary arterial hypertensionProtein kinasePhosphoproteomic analysisIdiopathic PAHKinase 1Zinc finger transcription factorLung tissueFinger transcription factorCGMP-dependent protein kinase 1Casein kinase 2Regulation of BclMass spectrometry-based analysisProtein kinase 1Arterial hypertensionImmune regulationImportant regulatory roleIdiopathic pulmonary arterial hypertensionRhoGTPase signalingGSK3 alphaKinase networkPhosphorylation assaysTranscription factorsBioinformatics analysisGSK3 beta
2017
Rice calcium‐dependent protein kinase OsCPK17 targets plasma membrane intrinsic protein and sucrose‐phosphate synthase and is required for a proper cold stress response
Almadanim MC, Alexandre BM, Rosa MTG, Sapeta H, Leitão AE, Ramalho JC, Lam TT, Negrão S, Abreu IA, Oliveira MM. Rice calcium‐dependent protein kinase OsCPK17 targets plasma membrane intrinsic protein and sucrose‐phosphate synthase and is required for a proper cold stress response. Plant Cell & Environment 2017, 40: 1197-1213. PMID: 28102545, DOI: 10.1111/pce.12916.Peer-Reviewed Original ResearchConceptsCalcium-dependent protein kinaseCold stress responseStress responsePlasma membrane intrinsic proteinsMembrane intrinsic proteinsPlant tolerance mechanismsStress-inducible genesSucrose-phosphate synthaseIntrinsic proteinsPhosphoproteomic approachCalcium-dependent mannerOsCPK17Kinase assaysSignal transductionProtein kinaseRice linesNitrogen metabolismSugar metabolismOsmotic regulationGene expressionTolerance mechanismsKey messengerMembrane channelsMost membersDirect interaction