2023
Multi-omics profiling reveals cellular pathways and functions regulated by ALDH1B1 in colon cancer cells
Wang Y, Popovic Z, Charkoftaki G, Garcia-Milian R, Lam T, Thompson D, Chen Y, Vasiliou V. Multi-omics profiling reveals cellular pathways and functions regulated by ALDH1B1 in colon cancer cells. Chemico-Biological Interactions 2023, 384: 110714. PMID: 37716420, PMCID: PMC10807983, DOI: 10.1016/j.cbi.2023.110714.Peer-Reviewed Original ResearchColon cancer cellsCellular stress response pathwaysStress response pathwaysMulti-omics analysisCancer cellsSecond messenger signalingMulti-omics profilingNew molecular informationFunctional annotationCellular functionsResponse pathwaysKinase signalingCellular pathwaysColon adenocarcinoma cell lineHuman colon adenocarcinoma cell lineApoptosis signalingEnrichment analysisAldehyde dehydrogenase 1B1Molecular signaturesAdenocarcinoma cell lineMolecular informationSignalingNovel targetProtein expressionCell lines
2022
Identification of growth hormone receptor as a relevant target for precision medicine in low‐EGFR expressing glioblastoma
Verreault M, Vilchis I, Rosenberg S, Lemaire N, Schmitt C, Guehennec J, Royer‐Perron L, Thomas J, Lam TT, Dingli F, Loew D, Ducray F, Paris S, Carpentier C, Marie Y, Laigle‐Donadey F, Rousseau A, Pigat N, Boutillon F, Bielle F, Mokhtari K, Frank SJ, de Reyniès A, Hoang‐Xuan K, Sanson M, Goffin V, Idbaih A. Identification of growth hormone receptor as a relevant target for precision medicine in low‐EGFR expressing glioblastoma. Clinical And Translational Medicine 2022, 12: e939. PMID: 35808822, PMCID: PMC9270581, DOI: 10.1002/ctm2.939.Peer-Reviewed Original ResearchConceptsEpidermal growth factor receptorGrowth hormone receptorPatient-derived cell linesOncogenic mechanismsGene expression profilesCell linesGain of functionHormone receptorsExpression of proteinsCellular movementGrowth factor receptorHuman GBM samplesExpression profilesCell migrationCommon oncogenic mechanismThird of patientsDistinct molecular subsetsGBM samplesPromoter hypermethylationNew therapeutic approachesFactor receptorCell proliferationPharmacological inhibitionRelevant targetsOverexpressionProteomic characterization of post-mortem human brain tissue following ultracentrifugation-based subcellular fractionation
Kandigian SE, Ethier EC, Kitchen RR, Lam TT, Arnold SE, Carlyle BC. Proteomic characterization of post-mortem human brain tissue following ultracentrifugation-based subcellular fractionation. Brain Communications 2022, 4: fcac103. PMID: 35611312, PMCID: PMC9123841, DOI: 10.1093/braincomms/fcac103.Peer-Reviewed Original ResearchProteomic characterizationSubcellular fractionationTissue cell type compositionMultiple cellular organellesPost-mortem human brain tissueMajority of proteinsThousands of proteinsCell type compositionHuman brain tissueSame biological sampleSpatial proteomicsProtein functionProtein localizationOrganellar markersCellular organellesCellular localizationDrug targetsSubcellular levelOrganellesAbundant organellesCentrifugation fractionsProteinCell linesDisease mechanismsMembrane breakdown
2013
Palmitoylation of Superoxide Dismutase 1 (SOD1) Is Increased for Familial Amyotrophic Lateral Sclerosis-linked SOD1 Mutants*
Antinone SE, Ghadge GD, Lam TT, Wang L, Roos RP, Green WN. Palmitoylation of Superoxide Dismutase 1 (SOD1) Is Increased for Familial Amyotrophic Lateral Sclerosis-linked SOD1 Mutants*. Journal Of Biological Chemistry 2013, 288: 21606-21617. PMID: 23760509, PMCID: PMC3724620, DOI: 10.1074/jbc.m113.487231.Peer-Reviewed Original ResearchMeSH KeywordsAmyotrophic Lateral SclerosisAnimalsBlotting, WesternCell Line, TumorCell MembraneCysteineDisulfidesHEK293 CellsHumansLipoylationLuminescent ProteinsMass SpectrometryMiceMice, TransgenicMutationNeuronsOxidation-ReductionProtein Processing, Post-TranslationalSpinal CordSuperoxide DismutaseSuperoxide Dismutase-1ConceptsWild-type SOD1Familial amyotrophic lateral sclerosisSuperoxide dismutase 1Copper chaperoneCysteine mutagenesisReversible post-translational modificationAcyl-biotin exchangeDisulfide bondingPost-translational modificationsMass spectrometryWild-type superoxide dismutase 1PalmitoylationSOD1 maturationMotor neuron cell lineProtein structureSOD1 mutantsNeuron cell lineAmyotrophic lateral sclerosisZn-superoxide dismutaseHEK cellsResidues 6ChaperonesCell linesMutagenesisDismutase 1
2011
Complex interactions in EML cell stimulation by stem cell factor and IL-3
Ye ZJ, Gulcicek E, Stone K, Lam T, Schulz V, Weissman SM. Complex interactions in EML cell stimulation by stem cell factor and IL-3. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 4882-4887. PMID: 21383156, PMCID: PMC3064389, DOI: 10.1073/pnas.1018002108.Peer-Reviewed Original ResearchConceptsStem cell factorHematopoietic precursor cell lineIL-3Cell factorPrecursor cell lineIL-3 receptorEML cellsTyrosine phosphorylationSubset of cellsSCF receptorDirect interactionCell linesMixed populationRelative levelsCell stimulationCellsComplex interactionsHeterogeneous mixtureReceptorsPhosphorylationLymphoid cells