2022
Impact of delivery rate on the acute response to intravenous nicotine: A human laboratory study with implications for regulatory science
De Aquino JP, MacLean RR, Gueorguieva R, DeVito EE, Eid T, Sofuoglu M. Impact of delivery rate on the acute response to intravenous nicotine: A human laboratory study with implications for regulatory science. Addiction Biology 2022, 27: e13161. PMID: 35229960, PMCID: PMC8903077, DOI: 10.1111/adb.13161.Peer-Reviewed Original ResearchConceptsAbuse potentialPositive subjective drug effectsSubjective drug effectsHuman laboratory studiesPositive subjective effectsAttention functionSmoking urgesOvernight abstinencePotential therapeutic effectsEffects of nicotineHeart rate increaseDelivery rateDependent smokersExperimental sessionsSubjective effectsNicotine dependenceIntravenous nicotineBlood pressureSaline infusionAcute effectsTherapeutic effectHeart rateDrug effectsAcute responseCrossover design
2021
Increased branched‐chain amino acids at baseline and hours before a spontaneous seizure in the human epileptic brain
Ong C, Damisah EC, Gruenbaum SE, Dhaher R, Deng Y, Sandhu MRS, Zaveri HP, Spencer DD, Eid T. Increased branched‐chain amino acids at baseline and hours before a spontaneous seizure in the human epileptic brain. Epilepsia 2021, 62: e88-e97. PMID: 33949690, PMCID: PMC11259155, DOI: 10.1111/epi.16920.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAmino Acids, Branched-ChainBrain ChemistryChildChild, PreschoolChromatography, High Pressure LiquidDrug Resistant EpilepsyElectrocorticographyElectroencephalographyEpilepsies, PartialExtracellular SpaceFemaleGlutamic AcidHumansIsoleucineMaleMicrodialysisMiddle AgedSeizuresTandem Mass SpectrometryYoung AdultConceptsSpontaneous seizuresDrug-resistant focal epilepsyExtracellular brain glutamateHuman epileptic brainBranched-chain amino acidsBranched chain amino acids valineRefractory epilepsyBrain microdialysisGlutamatergic neurotransmissionFocal epilepsySeizure onsetBrain glutamateEpileptic brainHuman patientsSeizuresBrain regionsDialysis samplesMammalian targetEpilepsyLiquid chromatography-tandem mass spectrometryBaselineBCAAIntracranial electroencephalographyChromatography-tandem mass spectrometryPatientsThreshold dose for intravenous nicotine self-administration in young adult non-dependent smokers
MacLean RR, DeVito EE, Eid T, Parida S, Gueorguieva R, Sofuoglu M. Threshold dose for intravenous nicotine self-administration in young adult non-dependent smokers. Psychopharmacology 2021, 238: 2083-2090. PMID: 33796907, PMCID: PMC8715498, DOI: 10.1007/s00213-021-05833-8.Peer-Reviewed Original ResearchConceptsDose of nicotineNicotine reinforcementHigh doseSubjective effectsHuman laboratory studiesYoung adult smokersNicotine self-administration modelNon-dependent smokersOvernight nicotine abstinenceEffects modelSelf-administration modelSelf-administration procedureNicotine doseAdult smokersMethodsYoung adultsObjectivesThe current studyNicotine abstinenceNicotine addictionHigh dosesLow dosesPlaceboThreshold doseReinforcement thresholdTobacco productsDose
2006
Changes in glial glutamate transporters in human epileptogenic hippocampus: Inadequate explanation for high extracellular glutamate during seizures
Bjørnsen LP, Eid T, Holmseth S, Danbolt NC, Spencer DD, de Lanerolle NC. Changes in glial glutamate transporters in human epileptogenic hippocampus: Inadequate explanation for high extracellular glutamate during seizures. Neurobiology Of Disease 2006, 25: 319-330. PMID: 17112731, DOI: 10.1016/j.nbd.2006.09.014.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAmino Acid Transport System X-AGAstrocytesChildChild, PreschoolDown-RegulationEpilepsyEpilepsy, Temporal LobeExcitatory Amino Acid Transporter 1Excitatory Amino Acid Transporter 2Extracellular FluidFemaleGlutamic AcidHippocampusHumansImmunohistochemistryMaleMicroscopy, Electron, TransmissionMiddle AgedUp-RegulationConceptsTemporal lobe epilepsySclerotic hippocampusNeuronal lossExtracellular glutamateGlial glutamate transporters GLASTHigh extracellular glutamate levelsGroups of hippocampiHuman epileptogenic hippocampusLess neuronal lossExtracellular glutamate levelsGlutamate transporter GLASTGlial glutamate transportersVivo dialysis studyHigh extracellular glutamateSuch compensatory changesEpileptogenic hippocampusHippocampal sclerosisLobe epilepsySclerotic groupSeizure focusGlutamate levelsWeak immunoreactivityGLT-1Glutamate transportersHippocampus
2004
Loss of glutamine synthetase in the human epileptogenic hippocampus: possible mechanism for raised extracellular glutamate in mesial temporal lobe epilepsy
Eid T, Thomas M, Spencer D, Rundén-Pran E, Lai J, Malthankar G, Kim J, Danbolt N, Ottersen O, de Lanerolle N. Loss of glutamine synthetase in the human epileptogenic hippocampus: possible mechanism for raised extracellular glutamate in mesial temporal lobe epilepsy. The Lancet 2004, 363: 28-37. PMID: 14723991, DOI: 10.1016/s0140-6736(03)15166-5.Peer-Reviewed Original ResearchConceptsMesial temporal lobe epilepsyTemporal lobe epilepsyMTLE hippocampiLobe epilepsyExtracellular glutamateHigh extracellular glutamate concentrationsAnteromedial temporal lobeHuman epileptogenic hippocampusExtracellular glutamate accumulationResolution of seizuresExtracellular glutamate concentrationGlial glutamate transportersMeans of immunohistochemistryEpileptogenic hippocampusSurgical resectionSeizure generationGlutamate homeostasisTemporal lobeAstroglial proliferationEpileptic seizuresGlutamate transportersHippocampusTherapeutic interventionsGlutamate concentrationWestern blot