2010
Shear stress-induced changes of membrane transporter localization and expression in mouse proximal tubule cells
Duan Y, Weinstein A, Weinbaum S, Wang T. Shear stress-induced changes of membrane transporter localization and expression in mouse proximal tubule cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 21860-21865. PMID: 21106755, PMCID: PMC3003103, DOI: 10.1073/pnas.1015751107.Peer-Reviewed Original ResearchConceptsProximal tubule cellsMouse proximal tubuleNa/K-ATPaseTubule cellsApical NHE3Proximal tubulesMouse proximal tubule cellsFSS-induced changesApical plasma membraneV-ATPaseAmount of protein expressionBasolateral membraneWestern blot analysisNHE3Volume reabsorptionTransporter expressionBasolateral transportProtein expressionMPT cellsShear stress-induced changesVacuolar H(+)-ATPaseBlock microtubule polymerizationControl cellsModulate cellular functionsTransporter localization
2003
Intracellular Na concentration and Rb uptake in proximal convoluted tubule cells and abundance of Na/K-ATPase α1-subunit in NHE3−/− mice
Beck F, Neuhofer W, Dörge A, Giebisch G, Wang T. Intracellular Na concentration and Rb uptake in proximal convoluted tubule cells and abundance of Na/K-ATPase α1-subunit in NHE3−/− mice. Pflügers Archiv - European Journal Of Physiology 2003, 446: 100-105. PMID: 12690468, DOI: 10.1007/s00424-002-1001-z.Peer-Reviewed Original ResearchConceptsNhe3-/- miceCell Na concentrationIntracellular Na concentrationProximal convoluted tubulesNhe3-/-Proximal convoluted tubule cellsNa/K-ATPaseNa/H exchanger isoform 3Rb uptakeConvoluted tubulesExpression of Na/K-ATPaseApical Na entryExchanger isoform 3Intracellular Cl- concentrationNa/K-ATPase activityDistal convoluted tubuleConvoluted tubule cellsApical NHE3NHE3 deficiencyNa entryIntercalated cellsWestern blot analysisNa concentrationTubule cellsRenal cortex