2016
Flow-activated proximal tubule function underlies glomerulotubular balance.
Du Z, Duan Y, Yan Q, Weinbaum S, Weinstein A, Wang T. Flow-activated proximal tubule function underlies glomerulotubular balance. 2016, 46: 105-117. PMID: 31105444, PMCID: PMC6519745.Peer-Reviewed Original ResearchProximal tubulesModulating Na<sup>+</sup>Isolated perfused proximal tubulesTubule cell volumeExchanger isoform 3Proximal tubule cellsProximal tubule functionPerfused proximal tubulesH-ATPase activityMouse tubulesNHE3 expressionStimulate sodiumApical membraneTubule functionBicarbonate absorptionTubule cellsReceptor-mediatedGlomerulotubular balanceMouse kidneyIsoform 3H-ATPaseCellular mechanismsTubulesTransporter densityNHE3
2013
REGULATION OF GLOMERULOTUBULAR BALANCE IV: IMPLICATION OF AQUAPORIN 1 IN FLOW‐DEPENDENT TRANSPORT AND CELL VOLUME
Du Z, Yan Q, Weinbaum S, Weinstein A, Wang T. REGULATION OF GLOMERULOTUBULAR BALANCE IV: IMPLICATION OF AQUAPORIN 1 IN FLOW‐DEPENDENT TRANSPORT AND CELL VOLUME. The FASEB Journal 2013, 27: 912.27-912.27. DOI: 10.1096/fasebj.27.1_supplement.912.27.Peer-Reviewed Original ResearchAQP-1Proximal tubulesKO miceWild-typeMicroperfused in vitroTransepithelial osmotic gradientHCO-3 transportWT miceNo significant differencePeritubular transportHCO-3 absorptionVolume reabsorptionPeritubular membraneWater reabsorptionAquaporin-1Mouse kidneyMicePerfusion rateH-ATPaseSignificant differenceTubulesReabsorptionOsmotic gradientCell volumePerfusion
2012
Regulation of glomerulotubular balance. II. Impact of angiotensin II on flow-dependent transport
Du Z, Wan L, Yan Q, Weinbaum S, Weinstein A, Wang T. Regulation of glomerulotubular balance. II. Impact of angiotensin II on flow-dependent transport. American Journal Of Physiology. Renal Physiology 2012, 303: f1507-f1516. PMID: 22952281, PMCID: PMC3532483, DOI: 10.1152/ajprenal.00277.2012.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAngiotensin II Type 1 Receptor BlockersAnimalsBicarbonatesBiological TransportEnzyme InhibitorsFemaleHemostasisIn Vitro TechniquesKidney GlomerulusKidney TubulesLosartanMacrolidesMiceMice, KnockoutModels, AnimalProton-Translocating ATPasesReceptor, Angiotensin, Type 1SodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsNa(+)-H(+) exchanger 3Mouse tubulesImpact of angiotensin IIH-ATPaseMicroperfused in vitroAT(2) receptorsAT(1A) receptorFlow-mediated changesH-ATPase activityParacellular Cl(-Receptor availabilityAngiotensin IIAT(1ARegulate Na(+Increased Na(+Local angiotensinBasolateral transportJ(HCO3Mouse kidneyNet fluidInhibitor bafilomycinProtein levelsMiceReceptorsTubules
2006
Flow-activated transport events along the nephron
Wang T. Flow-activated transport events along the nephron. Current Opinion In Nephrology & Hypertension 2006, 15: 530-536. PMID: 16914967, DOI: 10.1097/01.mnh.0000242180.46362.c4.Peer-Reviewed Original ResearchConceptsBrush-border microvilliProximal tubulesCultured proximal tubule cellsProximal tubules in vitroGlomerular filtration rateProximal tubule cellsBrush-borderH-ATPase activityTubules in vitroNHE3 activityHCO3- absorptionENOS translocationTubule cellsFiltration rateMg2+ transportReceptor localizationSignal transduction mechanismsH-ATPaseHormonal regulationCa2+NephronTubulesResponse to flowTransduction mechanismsIntact actin cytoskeleton
2005
Axial flow modulates proximal tubule NHE3 and H-ATPase activities by changing microvillus bending moments
Du Z, Yan Q, Duan Y, Weinbaum S, Weinstein A, Wang T. Axial flow modulates proximal tubule NHE3 and H-ATPase activities by changing microvillus bending moments. American Journal Of Physiology. Renal Physiology 2005, 290: f289-f296. PMID: 16144961, DOI: 10.1152/ajprenal.00255.2005.Peer-Reviewed Original ResearchConceptsProximal tubules in vitroHCO3- absorptionTubules in vitroBrush-border microvilliHCO3- permeabilityH(+)-ATPase inhibitor bafilomycinInhibition of NHE3Proximal tubule NHE3Transepithelial potential differenceH-ATPase activityLuminal cell membraneH(+)-ATPaseTransepithelial PDNa+ absorptionParacellular permeabilityH(+)-ATPase activityInhibitor EIPAMembrane transport activityProximal tubulesHCO3- reabsorptionNHE3HCO3- transportH-ATPaseBicarbonate transportPermeability changes
1993
Renal bicarbonate reabsorption in the rat. IV. Bicarbonate transport mechanisms in the early and late distal tubule.
Wang T, Malnic G, Giebisch G, Chan Y. Renal bicarbonate reabsorption in the rat. IV. Bicarbonate transport mechanisms in the early and late distal tubule. Journal Of Clinical Investigation 1993, 91: 2776-2784. PMID: 8390489, PMCID: PMC443344, DOI: 10.1172/jci116519.Peer-Reviewed Original ResearchConceptsLate distal tubuleBicarbonate absorptionDistal tubulesTubule segmentsBicarbonate transportH-ATPaseH-K-ATPase inhibitorsRate of bicarbonate absorptionEthyl-isopropylamilorideFluid absorptionNa(+)-H+ exchangeK(+)-H+ exchangeEarly distal tubuleBicarbonate transport mechanismDistal tubule segmentsRenal bicarbonate reabsorptionLow-K dietLuminal bicarbonate concentrationBicarbonate reabsorptionBicarbonate secretionBicarbonate concentrationH+ secretionMicroperfusion experimentsTubulesK diet