2022
Inhibition of renalase drives tumour rejection by promoting T cell activation
Guo X, Jessel S, Qu R, Kluger Y, Chen TM, Hollander L, Safirstein R, Nelson B, Cha C, Bosenberg M, Jilaveanu LB, Rimm D, Rothlin CV, Kluger HM, Desir GV. Inhibition of renalase drives tumour rejection by promoting T cell activation. European Journal Of Cancer 2022, 165: 81-96. PMID: 35219026, PMCID: PMC8940682, DOI: 10.1016/j.ejca.2022.01.002.Peer-Reviewed Original ResearchConceptsPD-1 inhibitorsMurine melanoma modelMelanoma-bearing miceMelanoma modelTumor microenvironmentTumor rejectionCell death protein 1 (PD-1) inhibitorsAnti-PD-1 activityEnhanced T cell infiltrationT cell-dependent fashionMelanoma cellsMelanoma tumor regressionPreclinical melanoma modelsT cell infiltrationNatural killer cellsForkhead box P3Expression of IFNγWild-type miceProtein 1 inhibitorT cell activationTumor cell contentWild-type melanoma cellsCD4 cellsAdvanced melanomaAntibody treatment
2021
Renalase is a novel tissue and serological biomarker in pancreatic ductal adenocarcinoma
Gao Y, Wang M, Guo X, Hu J, Chen TM, Finn S, Lacy J, Kunstman JW, H. C, Bellin MD, Robert ME, Desir GV, Gorelick FS. Renalase is a novel tissue and serological biomarker in pancreatic ductal adenocarcinoma. PLOS ONE 2021, 16: e0250539. PMID: 34587190, PMCID: PMC8480607, DOI: 10.1371/journal.pone.0250539.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overBiomarkers, TumorCarcinoma, Pancreatic DuctalCase-Control StudiesFemaleGene Expression Regulation, NeoplasticHumansMaleMiddle AgedMonoamine OxidaseNeoplasm GradingPancreatic NeoplasmsPrognosisProspective StudiesRetrospective StudiesSurvival AnalysisUp-RegulationYoung AdultConceptsPlasma renalase levelsBorderline resectable PDACRenalase levelsPDAC precursor lesionsOverall survivalPDAC tissuesTumor characteristicsResectable PDACChronic pancreatitisPrecursor lesionsNormal pancreasPancreatic ductal adenocarcinoma growthAdvanced tumor characteristicsVaried clinical stagesWorse tumor characteristicsNode-positive diseasePancreatic ductal adenocarcinomaNormal pancreatic headSpindle-shaped cellsPlasma renalaseRenalase expressionUnderwent resectionAbdominal traumaPancreatic headPositive disease
2013
Development of modified siRNA molecules incorporating 5-fluoro-2′-deoxyuridine residues to enhance cytotoxicity
Wu SY, Chen TM, Gmeiner WH, Chu E, Schmitz JC. Development of modified siRNA molecules incorporating 5-fluoro-2′-deoxyuridine residues to enhance cytotoxicity. Nucleic Acids Research 2013, 41: 4650-4659. PMID: 23449220, PMCID: PMC3632118, DOI: 10.1093/nar/gkt120.Peer-Reviewed Original ResearchConceptsTS proteinMultiple DNA damage repairCovalent inhibitory ternary complexNovel drug development approachDNA damage repairInhibitory ternary complexRNA stabilityDamage repairApoptotic pathwayHuman diseasesWatson-Crick base pairingPrecise fateSiRNAsControl siRNAsBase pairingTernary complexRNA expressionThymidylate synthaseMessenger RNA expressionDrug development approachesCytotoxic nucleosidesInhibitor compoundsNucleotidesSiRNAProtein
2004
Translational autoregulation of thymidylate synthase and dihydrofolate reductase.
Tai N, Schmitz JC, Liu J, Lin X, Bailly M, Chen TM, Chu E. Translational autoregulation of thymidylate synthase and dihydrofolate reductase. Frontiers In Bioscience-Landmark 2004, 9: 2521-6. PMID: 15353304, DOI: 10.2741/1413.Peer-Reviewed Original ResearchConceptsSpecific RNA-protein interactionsRNA-protein interactionsVivo experimental model systemsAutoregulatory feedback mechanismFolate-dependent enzymesTranslational repressionTranslational autoregulationCognate mRNADNA repairMolecular basisExperimental model systemTS proteinDHFR mRNAMolecular elementsCellular drug resistanceNucleotide precursorsFunctional consequencesDihydrofolate reductaseDHFR expressionAutoregulatory controlDNA synthesisEssential roleEnzyme catalysisThymidylate synthaseModel systemSmall Interfering Double-Stranded RNAs as Therapeutic Molecules to Restore Chemosensitivity to Thymidylate Synthase Inhibitor Compounds
Schmitz JC, Chen TM, Chu E. Small Interfering Double-Stranded RNAs as Therapeutic Molecules to Restore Chemosensitivity to Thymidylate Synthase Inhibitor Compounds. Cancer Research 2004, 64: 1431-1435. PMID: 14973067, DOI: 10.1158/0008-5472.can-03-1203.Peer-Reviewed Original ResearchConceptsTS expressionRKO cellsDose-dependent inhibitionCellular drug resistanceHuman colon cancer RKO cellsSynthase inhibitorColon cancer RKO cellsTherapeutic potentialDrug resistanceRNA interferenceMRNA levelsCell linesI. InhibitionProtein inductionSynthase mRNAChemosensitivityRaltitrexedDouble-stranded RNAInhibitor compoundsTherapeutic moleculesThymidylate synthase mRNATS mRNAPost-transcriptional mechanismsSiRNAEffective inhibitor
2001
Translational Regulation as a Novel Mechanism for the Development of Cellular Drug Resistance
Schmitz J, Liu J, Lin X, Chen T, Yan W, Tai N, Gollerkeri A, Chu E. Translational Regulation as a Novel Mechanism for the Development of Cellular Drug Resistance. Cancer And Metastasis Reviews 2001, 20: 33-41. PMID: 11831645, DOI: 10.1023/a:1013100306315.Peer-Reviewed Original Research