2013
Development of modified siRNA molecules incorporating 5-fluoro-2′-deoxyuridine residues to enhance cytotoxicity
Wu SY, Chen TM, Gmeiner WH, Chu E, Schmitz JC. Development of modified siRNA molecules incorporating 5-fluoro-2′-deoxyuridine residues to enhance cytotoxicity. Nucleic Acids Research 2013, 41: 4650-4659. PMID: 23449220, PMCID: PMC3632118, DOI: 10.1093/nar/gkt120.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisCell Line, TumorDeoxyuridineHumansRNA, Small InterferingThymidylate SynthaseTransfectionConceptsTS proteinMultiple DNA damage repairCovalent inhibitory ternary complexNovel drug development approachDNA damage repairInhibitory ternary complexRNA stabilityDamage repairApoptotic pathwayHuman diseasesWatson-Crick base pairingPrecise fateSiRNAsControl siRNAsBase pairingTernary complexRNA expressionThymidylate synthaseMessenger RNA expressionDrug development approachesCytotoxic nucleosidesInhibitor compoundsNucleotidesSiRNAProtein
2004
Translational autoregulation of thymidylate synthase and dihydrofolate reductase.
Tai N, Schmitz JC, Liu J, Lin X, Bailly M, Chen TM, Chu E. Translational autoregulation of thymidylate synthase and dihydrofolate reductase. Frontiers In Bioscience-Landmark 2004, 9: 2521-6. PMID: 15353304, DOI: 10.2741/1413.Peer-Reviewed Original ResearchConceptsSpecific RNA-protein interactionsRNA-protein interactionsVivo experimental model systemsAutoregulatory feedback mechanismFolate-dependent enzymesTranslational repressionTranslational autoregulationCognate mRNADNA repairMolecular basisExperimental model systemTS proteinDHFR mRNAMolecular elementsCellular drug resistanceNucleotide precursorsFunctional consequencesDihydrofolate reductaseDHFR expressionAutoregulatory controlDNA synthesisEssential roleEnzyme catalysisThymidylate synthaseModel systemSmall Interfering Double-Stranded RNAs as Therapeutic Molecules to Restore Chemosensitivity to Thymidylate Synthase Inhibitor Compounds
Schmitz JC, Chen TM, Chu E. Small Interfering Double-Stranded RNAs as Therapeutic Molecules to Restore Chemosensitivity to Thymidylate Synthase Inhibitor Compounds. Cancer Research 2004, 64: 1431-1435. PMID: 14973067, DOI: 10.1158/0008-5472.can-03-1203.Peer-Reviewed Original ResearchConceptsTS expressionRKO cellsDose-dependent inhibitionCellular drug resistanceHuman colon cancer RKO cellsSynthase inhibitorColon cancer RKO cellsTherapeutic potentialDrug resistanceRNA interferenceMRNA levelsCell linesI. InhibitionProtein inductionSynthase mRNAChemosensitivityRaltitrexedDouble-stranded RNAInhibitor compoundsTherapeutic moleculesThymidylate synthase mRNATS mRNAPost-transcriptional mechanismsSiRNAEffective inhibitor