2013
A General Approach for Controlling Transcription and Probing Epigenetic Mechanisms: Application to the Cd4 Locus
Wan M, Kaundal R, Huang H, Zhao J, Yang X, Chaiyachati BH, Li S, Chi T. A General Approach for Controlling Transcription and Probing Epigenetic Mechanisms: Application to the Cd4 Locus. The Journal Of Immunology 2013, 190: 737-747. PMID: 23293358, PMCID: PMC3744393, DOI: 10.4049/jimmunol.1201278.Peer-Reviewed Original Research
2008
A novel genetic strategy reveals unexpected roles of the Swi–Snf–like chromatin-remodeling BAF complex in thymocyte development
Jani A, Wan M, Zhang J, Cui K, Wu J, Preston-Hurlburt P, Khatri R, Zhao K, Chi T. A novel genetic strategy reveals unexpected roles of the Swi–Snf–like chromatin-remodeling BAF complex in thymocyte development. Journal Of Experimental Medicine 2008, 205: 2813-2825. PMID: 18955569, PMCID: PMC2585832, DOI: 10.1084/jem.20080938.Peer-Reviewed Original ResearchConceptsPoint mutantsUnexpected roleImportant gene functionsThymocyte developmentNovel genetic strategyPoint mutationsEarly thymocyte developmentMammalian geneticsChromatin templatesSWI-SNFBAF complexGene functionATPase subunitsDeletion mutantsFactor complexCD4 locusTarget genesGenetic strategiesCD4 activationMutantsNovel activityPhysical interactionDeletionBRGMutationsNucleoprotein structure of the CD4 locus: Implications for the mechanisms underlying CD4 regulation during T cell development
Yu M, Wan M, Zhang J, Wu J, Khatri R, Chi T. Nucleoprotein structure of the CD4 locus: Implications for the mechanisms underlying CD4 regulation during T cell development. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 3873-3878. PMID: 18322012, PMCID: PMC2268827, DOI: 10.1073/pnas.0800810105.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD4 AntigensChromatin ImmunoprecipitationEnhancer Elements, GeneticGene DeletionGene Expression RegulationHistonesLymphocyte SubsetsMiceMice, Inbred C57BLModels, BiologicalNucleoproteinsPromoter Regions, GeneticProtein BindingSilencer Elements, TranscriptionalT-LymphocytesTranscription FactorsConceptsT cell developmentCell developmentNucleoprotein structuresCD4 locusHeterochromatin-like structureOpen chromatin configurationStage-specific mannerSame regulatory elementsDP cellsRepression patternsTranscription activatorCD4 silencerP300 recruitmentChromatin configurationRegulatory elementsDN cellsMolecular basisMolecular mechanismsCD4 enhancerCD4 transcriptionMature T cellsCD4 regulationCD4 geneCD4 promoterSilencer
1998
Defects in actin-cap formation in Vav-deficient mice implicate an actin requirement for lymphocyte signal transduction
Holsinger L, Graef I, Swat W, Chi T, Bautista D, Davidson L, Lewis R, Alt F, Crabtree G. Defects in actin-cap formation in Vav-deficient mice implicate an actin requirement for lymphocyte signal transduction. Current Biology 1998, 8: 563-573. PMID: 9601640, DOI: 10.1016/s0960-9822(98)70225-8.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCell Cycle ProteinsCytoskeletonDNA-Binding ProteinsHumansJurkat CellsMiceNFATC Transcription FactorsNuclear ProteinsProto-Oncogene ProteinsProto-Oncogene Proteins c-vavReceptor-CD3 Complex, Antigen, T-CellReceptors, Antigen, T-CellSignal TransductionT-LymphocytesTranscription FactorsTranscription, GeneticConceptsMitogen-activated protein kinaseCap formationActin polymerizationGuanine nucleotide exchange factorsJun N-terminal kinaseTranscription factor NF-ATc1Vav-deficient miceActin-dependent pathwayStress-activated kinasesGrowth regulatory signalsActin cap formationAntigen receptor signalingEgr-1 geneLymphocyte signal transductionN-terminal kinaseAntigen-receptor interactionActin cytoskeletonExchange factorTranscriptional inductionSignal transductionDependent transcriptionProtein kinaseGTPase RacRegulatory signalsNull mutation