2022
PPARγ phase separates with RXRα at PPREs to regulate target gene expression
Li Z, Luo L, Yu W, Li P, Ou D, Liu J, Ma H, Sun Q, Liang A, Huang C, Chi T, Huang X, Zhang Y. PPARγ phase separates with RXRα at PPREs to regulate target gene expression. Cell Discovery 2022, 8: 37. PMID: 35473936, PMCID: PMC9043196, DOI: 10.1038/s41421-022-00388-0.Peer-Reviewed Original ResearchPPAR response elementNuclear condensatesTranscriptional activationPPRE siteZinc finger motifsDNA binding domainsKey transcription activatorSpecific transcriptional activationTarget gene expressionPPARγ/RXRαRetinoid X receptor αPPARγ target genesFinger motifPhase-separated dropletsTranscription activatorTranscriptional responseObligate heterodimersTarget genesX receptor αBinding domainsGene expressionResponse elementPeroxisome proliferator-activated receptorNovel mechanismProliferator-activated receptor
2012
Essential Roles of the Chromatin Remodeling Factor Brg1 in Spermatogenesis in Mice1
Wang J, Gu H, Lin H, Chi T. Essential Roles of the Chromatin Remodeling Factor Brg1 in Spermatogenesis in Mice1. Biology Of Reproduction 2012, 86: 186, 1-10. PMID: 22495890, PMCID: PMC3386149, DOI: 10.1095/biolreprod.111.097097.Peer-Reviewed Original ResearchConceptsMeiotic recombinationSomatic cellsDNA repairMeiotic sex chromosome inactivationChromatin Remodeling Factor BRG1Sex chromosome inactivationChromatin-remodeling complexBAF chromatin-remodeling complexImpaired homologous recombinationEssential roleRole of BRG1Chromatin regulationChromatin structureFactor BRG1Male germlineMammalian spermatogenesisSpatiotemporal regulationChromosome inactivationCatalytic subunitHomologous recombinationMidpachytene stageBRG1Expression patternsGene expressionGerm cells
2009
Induction of TLR4-target genes entails calcium/calmodulin-dependent regulation of chromatin remodeling
Lai D, Wan M, Wu J, Preston-Hurlburt P, Kushwaha R, Grundström T, Imbalzano AN, Chi T. Induction of TLR4-target genes entails calcium/calmodulin-dependent regulation of chromatin remodeling. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 1169-1174. PMID: 19164553, PMCID: PMC2633529, DOI: 10.1073/pnas.0811274106.Peer-Reviewed Original ResearchConceptsCalcium/CaMChromatin remodelingBAF complexSWI/SNF-like BAF chromatinTarget genesDominant negative mutantCalcium/calmodulinCalmodulin-dependent regulationHMG domainBAF chromatinCaM-binding peptideNegative mutantGene expressionCaM antagonistsGenesToll-like receptor 4ChromatinRegulationRemodelingRecruitmentComplexesBAF57RNAiMutantsInduction
2004
A BAF-centred view of the immune system
Chi T. A BAF-centred view of the immune system. Nature Reviews Immunology 2004, 4: 965-977. PMID: 15573131, DOI: 10.1038/nri1501.Peer-Reviewed Original ResearchConceptsChromatin-remodelling complexBAF complexRepressive chromatin structureStructure of chromatinHistone-DNA contactsTranscription factor complexPost-translational modificationsTranscriptional start siteIFN-β geneTranscriptional machineryChromatin structureTranscriptional activatorTranscriptional repressorHuman genesResponsive genesExpression of CIITAStart siteDNA sequencesFactor complexHistone octamerNucleosomesOwn replicationGene expressionGenomic DNAIFN-β expression