2014
Use of Imipenem To Detect KPC, NDM, OXA, IMP, and VIM Carbapenemase Activity from Gram-Negative Rods in 75 Minutes Using Liquid Chromatography-Tandem Mass Spectrometry
Kulkarni MV, Zurita AN, Pyka JS, Murray TS, Hodsdon ME, Peaper DR. Use of Imipenem To Detect KPC, NDM, OXA, IMP, and VIM Carbapenemase Activity from Gram-Negative Rods in 75 Minutes Using Liquid Chromatography-Tandem Mass Spectrometry. Journal Of Clinical Microbiology 2014, 52: 2500-2505. PMID: 24789180, PMCID: PMC4097713, DOI: 10.1128/jcm.00547-14.Peer-Reviewed Original Research
2013
Rapid detection of carbapenemase activity through monitoring ertapenem hydrolysis in Enterobacteriaceae with LCMS/MS
Peaper DR, Kulkarni MV, Tichy AN, Jarvis M, Murray TS, Hodsdon ME. Rapid detection of carbapenemase activity through monitoring ertapenem hydrolysis in Enterobacteriaceae with LCMS/MS. Bioanalysis 2013, 5: 147-157. PMID: 23330558, PMCID: PMC5753620, DOI: 10.4155/bio.12.310.Peer-Reviewed Original ResearchConceptsLife-threatening infectionsIll patientsGram-negative rodsDiagnostic testsExpression of carbapenemasesAvailable diagnostic testsRapid diagnostic testsRatios of metabolitesLC-MS/MSCarbapenemase activityHodge testCarbapenem resistanceCarbapenemase productionCarbapenem antibioticsΒ-lactam antibioticsThreshold cutoffDA increaseErtapenem hydrolysisLC-MS/MS methodPatientsClinical laboratoriesAntibioticsLCMS/MSInfectionPhysiological levels