2024
Novel muscle-derived extracellular matrix hydrogel promotes angiogenesis and neurogenesis in volumetric muscle loss
Chen Z, Huang Y, Xing H, Tseng T, Edelman H, Perry R, Kyriakides T. Novel muscle-derived extracellular matrix hydrogel promotes angiogenesis and neurogenesis in volumetric muscle loss. Matrix Biology 2024, 127: 38-47. PMID: 38325441, PMCID: PMC10958762, DOI: 10.1016/j.matbio.2024.02.001.Peer-Reviewed Original ResearchConceptsDecellularized extracellular matrixVolumetric muscle lossCharacterization of hydrogelsExtracellular matrix hydrogelTreat VML injuriesMuscle lossEnhancement of tissue repairRegenerative capacity of skeletal muscleMatrix hydrogelPromote muscle repairRegenerative capacityHydrogelsVolumetric muscle loss modelNative biomaterialsScar tissue formationEnhanced cell invasionCapacity of skeletal muscleMuscle repairTibialis anterior muscleClinical challengeBlood vessel formationReduce inflammationVML injuryLoss of functionSubcutaneous implantation
2019
Thrombospondin-2 regulates extracellular matrix production, LOX levels, and cross-linking via downregulation of miR-29
Calabro NE, Barrett A, Chamorro-Jorganes A, Tam S, Kristofik NJ, Xing H, Loye AM, Sessa WC, Hansen K, Kyriakides TR. Thrombospondin-2 regulates extracellular matrix production, LOX levels, and cross-linking via downregulation of miR-29. Matrix Biology 2019, 82: 71-85. PMID: 30876926, PMCID: PMC6710120, DOI: 10.1016/j.matbio.2019.03.002.Peer-Reviewed Original ResearchConceptsECM protein productionProtein productionLysyl oxidaseThrombospondin-2MiR-29Matricellular proteinQuantitative proteomics approachLevels of LOXExtracellular matrix productionProteomic approachECM proteinsMajor regulatorECM homeostasisExtracellular matrixMatrix productionProteinCollagen fibrillogenesisMiR-29 expressionFibrillar collagenImportant modulatorDependent processesLOX levelsRegulatorProductionHomeostasis
2018
Tunable Hydrogels Derived from Genetically Engineered Extracellular Matrix Accelerate Diabetic Wound Healing
Morris AH, Lee H, Xing H, Stamer DK, Tan M, Kyriakides TR. Tunable Hydrogels Derived from Genetically Engineered Extracellular Matrix Accelerate Diabetic Wound Healing. ACS Applied Materials & Interfaces 2018, 10: 41892-41901. PMID: 30424595, PMCID: PMC9996546, DOI: 10.1021/acsami.8b08920.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDiabetes Mellitus, ExperimentalExtracellular MatrixHydrogelsMiceMice, KnockoutNIH 3T3 CellsProtein EngineeringThrombospondinsWound Healing
2017
Improving in vivo outcomes of decellularized vascular grafts via incorporation of a novel extracellular matrix
Kristofik NJ, Qin L, Calabro NE, Dimitrievska S, Li G, Tellides G, Niklason LE, Kyriakides TR. Improving in vivo outcomes of decellularized vascular grafts via incorporation of a novel extracellular matrix. Biomaterials 2017, 141: 63-73. PMID: 28667900, PMCID: PMC5918415, DOI: 10.1016/j.biomaterials.2017.06.025.Peer-Reviewed Original ResearchConceptsUnmodified graftsVascular graftsCoronary bypass proceduresMechanical propertiesMural cell recruitmentBypass proceduresRat aortaMMP levelsCell recruitmentDecreased failure rateGraftPlatelet studiesGraft mechanical propertiesNative vesselsShelf vascular graftsSmall-diameter vascular graftsTime pointsExtracellular matrixDiameter vascular graftsTensile strengthYoung's modulusWT cellsPresent studySuture retentionFailure rateA hidden structural vulnerability in the thrombospondin-2 deficient aorta increases the propensity to intramural delamination
Bellini C, Kristofik NJ, Bersi MR, Kyriakides TR, Humphrey JD. A hidden structural vulnerability in the thrombospondin-2 deficient aorta increases the propensity to intramural delamination. Journal Of The Mechanical Behavior Of Biomedical Materials 2017, 71: 397-406. PMID: 28412645, PMCID: PMC8344363, DOI: 10.1016/j.jmbbm.2017.01.045.Peer-Reviewed Original ResearchThe host response to naturally-derived extracellular matrix biomaterials
Morris AH, Stamer DK, Kyriakides TR. The host response to naturally-derived extracellular matrix biomaterials. Seminars In Immunology 2017, 29: 72-91. PMID: 28274693, DOI: 10.1016/j.smim.2017.01.002.Peer-Reviewed Original ResearchMeSH KeywordsAcellular DermisAdaptive ImmunityAnimalsBiocompatible MaterialsExtracellular MatrixForeign-Body ReactionHumansHydrogelsPolymersRegenerative Medicine
2016
Impaired von Willebrand factor adhesion and platelet response in thrombospondin-2 knockout mice
Kristofik N, Calabro NE, Tian W, Meng A, MacLauchlan S, Wang Y, Breuer CK, Tellides G, Niklason LE, Kyriakides TR. Impaired von Willebrand factor adhesion and platelet response in thrombospondin-2 knockout mice. Blood 2016, 128: 1642-1650. PMID: 27471233, PMCID: PMC5034742, DOI: 10.1182/blood-2016-03-702845.Peer-Reviewed Original Research
2001
Altered Extracellular Matrix Remodeling and Angiogenesis in Sponge Granulomas of Thrombospondin 2-Null Mice
Kyriakides T, Zhu Y, Yang Z, Huynh G, Bornstein P. Altered Extracellular Matrix Remodeling and Angiogenesis in Sponge Granulomas of Thrombospondin 2-Null Mice. American Journal Of Pathology 2001, 159: 1255-1262. PMID: 11583953, PMCID: PMC1850515, DOI: 10.1016/s0002-9440(10)62512-6.Peer-Reviewed Original ResearchConceptsTSP2-null miceMatrix remodelingWild-type miceMatrix metalloproteinase-2Wild-type animalsExtracellular matrix remodelingModulators of angiogenesisFibrogenic responseImmunohistochemical analysisMetalloproteinase-2Minimal scarringMMP2 levelsSponge granulomaAngiogenesis inhibitorsMice displayVivo evidenceThrombospondin-2MiceGrowth factorImportant modulatorTissue invasionTSP2-nullWound healingAngiogenesisSignificant differencesRegulation of Angiogenesis and Matrix Remodeling by Localized, Matrix-Mediated Antisense Gene Delivery
Kyriakides T, Hartzel T, Huynh G, Bornstein P. Regulation of Angiogenesis and Matrix Remodeling by Localized, Matrix-Mediated Antisense Gene Delivery. Molecular Therapy 2001, 3: 842-849. PMID: 11407897, DOI: 10.1006/mthe.2001.0336.Peer-Reviewed Original Research
2000
Matricellular Proteins as Modulators of Cell–Matrix Interactions: Adhesive Defect in Thrombospondin 2-null Fibroblasts is a Consequence of Increased Levels of Matrix Metalloproteinase-2
Yang Z, Kyriakides T, Bornstein P. Matricellular Proteins as Modulators of Cell–Matrix Interactions: Adhesive Defect in Thrombospondin 2-null Fibroblasts is a Consequence of Increased Levels of Matrix Metalloproteinase-2. Molecular Biology Of The Cell 2000, 11: 3353-3364. PMID: 11029041, PMCID: PMC14997, DOI: 10.1091/mbc.11.10.3353.Peer-Reviewed Original ResearchConceptsCell-matrix interactionsAbnormal cell-matrix interactionsWild-type cellsTSP2-null miceAdhesive defectsComplex phenotypesCell spreadingMolecular mechanismsMutant fibroblastsProtein resultsMatrix proteinsStable transfectionMatrix metalloproteinasesMetalloproteinase-2Matricellular proteinAltered expressionTHBS2 geneNull miceAffinity chromatographyGelatinolytic assaysThrombospondin-2TSP2Matrix metalloproteinase-2FibroblastsLevels of MMP2
1999
Mice that lack the angiogenesis inhibitor, thrombospondin 2, mount an altered foreign body reaction characterized by increased vascularity
Kyriakides T, Leach K, Hoffman A, Ratner B, Bornstein P. Mice that lack the angiogenesis inhibitor, thrombospondin 2, mount an altered foreign body reaction characterized by increased vascularity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 4449-4454. PMID: 10200282, PMCID: PMC16352, DOI: 10.1073/pnas.96.8.4449.Peer-Reviewed Original Research