2020
The effect of compound kushen injection on cancer cells: Integrated identification of candidate molecular mechanisms
Cui J, Qu Z, Harata-Lee Y, Shen H, Aung TN, Wang W, Kortschak RD, Adelson DL. The effect of compound kushen injection on cancer cells: Integrated identification of candidate molecular mechanisms. PLOS ONE 2020, 15: e0236395. PMID: 32730293, PMCID: PMC7392229, DOI: 10.1371/journal.pone.0236395.Peer-Reviewed Original ResearchConceptsCore candidate genesCandidate pathwaysCandidate genesDifferential gene expression resultsGene expressionDNA replication/repairCompound Kushen InjectionTranscriptome-based analysisReplication/repairCancer cell linesEffect of CKITranscriptome data analysisCandidate molecular mechanismsCell cycle regulatorsMolecular changesCell linesGene expression resultsCell cycle phasesCell cycle arrestDE genesRegulatory relationshipsCycle regulatorsCell cycleMolecular mechanismsExpression results
2019
Fractional Deletion of Compound Kushen Injection Indicates Cytokine Signaling Pathways are Critical for its Perturbation of the Cell Cycle
Aung TN, Nourmohammadi S, Qu Z, Harata-Lee Y, Cui J, Shen HY, Yool AJ, Pukala T, Du H, Kortschak RD, Wei W, Adelson DL. Fractional Deletion of Compound Kushen Injection Indicates Cytokine Signaling Pathways are Critical for its Perturbation of the Cell Cycle. Scientific Reports 2019, 9: 14200. PMID: 31578346, PMCID: PMC6775143, DOI: 10.1038/s41598-019-50271-4.Peer-Reviewed Original ResearchConceptsCompound Kushen InjectionExperimental biology approachesEffect of CKIPlant secondary metabolitesCytokine Signaling PathwaysCKI activityBiology approachReconstitution approachGene expressionCell cyclePhenotype dataSignaling pathwaysSecondary metabolitesBreast cancer cell linesCancer cell linesKushen InjectionSingle major compoundCell linesApoptosisLiquid chromatography fractionationCell viabilityHigh-performance liquid chromatography fractionationMajor compoundsComplex fashionCandidate mechanism
2016
Identification of candidate anti-cancer molecular mechanisms of compound kushen injection using functional genomics
Qu Z, Cui J, Harata-Lee Y, Aung TN, Feng Q, Raison JM, Kortschak RD, Adelson DL. Identification of candidate anti-cancer molecular mechanisms of compound kushen injection using functional genomics. Oncotarget 2016, 7: 66003-66019. PMID: 27602759, PMCID: PMC5323210, DOI: 10.18632/oncotarget.11788.Peer-Reviewed Original ResearchConceptsCompound Kushen InjectionAnti-cancer molecular mechanismMolecular mechanismsHigh-throughput Illumina RNA-Seq technologyIllumina RNA-Seq technologyMCF-7 cellsKushen InjectionRNA-seq technologyEffect of CKITranscriptome analysis methodsLong non-coding RNAsPlant secondary metabolitesNon-coding RNAsDifferential expression analysisCell proliferationGene differential expression analysisMCF-7 human breast cancer cell lineDe novo identificationHuman breast cancer cell linesMCF-7 cell proliferationBreast cancer cell linesDose-dependent fashionAnnexin V/propidium iodideTraditional Chinese medicine preparationP53-independent mechanism