2023
Subsets of IFN Signaling Predict Response to Immune Checkpoint Blockade in Patients with Melanoma.
Horowitch B, Lee D, Ding M, Martinez-Morilla S, Aung T, Ouerghi F, Wang X, Wei W, Damsky W, Sznol M, Kluger H, Rimm D, Ishizuka J. Subsets of IFN Signaling Predict Response to Immune Checkpoint Blockade in Patients with Melanoma. Clinical Cancer Research 2023, 29: 2908-2918. PMID: 37233452, PMCID: PMC10524955, DOI: 10.1158/1078-0432.ccr-23-0215.Peer-Reviewed Original ResearchConceptsImmune checkpoint inhibitorsHuman melanoma cell linesMelanoma cell linesPD-L1Validation cohortYale-New Haven HospitalCombination of ipilimumabPD-L1 markersImmune checkpoint blockadePD-L1 biomarkerNew Haven HospitalSTAT1 levelsCell linesWestern blot analysisCheckpoint inhibitorsCheckpoint blockadeClinical responseOverall survivalImproved survivalResistance of cancersMetastatic melanomaMelanoma responsePredict responseTreatment responseDistinct patterns
2020
The effect of compound kushen injection on cancer cells: Integrated identification of candidate molecular mechanisms
Cui J, Qu Z, Harata-Lee Y, Shen H, Aung TN, Wang W, Kortschak RD, Adelson DL. The effect of compound kushen injection on cancer cells: Integrated identification of candidate molecular mechanisms. PLOS ONE 2020, 15: e0236395. PMID: 32730293, PMCID: PMC7392229, DOI: 10.1371/journal.pone.0236395.Peer-Reviewed Original ResearchConceptsCore candidate genesCandidate pathwaysCandidate genesDifferential gene expression resultsGene expressionDNA replication/repairCompound Kushen InjectionTranscriptome-based analysisReplication/repairCancer cell linesEffect of CKITranscriptome data analysisCandidate molecular mechanismsCell cycle regulatorsMolecular changesCell linesGene expression resultsCell cycle phasesCell cycle arrestDE genesRegulatory relationshipsCycle regulatorsCell cycleMolecular mechanismsExpression results
2019
Fractional Deletion of Compound Kushen Injection Indicates Cytokine Signaling Pathways are Critical for its Perturbation of the Cell Cycle
Aung TN, Nourmohammadi S, Qu Z, Harata-Lee Y, Cui J, Shen HY, Yool AJ, Pukala T, Du H, Kortschak RD, Wei W, Adelson DL. Fractional Deletion of Compound Kushen Injection Indicates Cytokine Signaling Pathways are Critical for its Perturbation of the Cell Cycle. Scientific Reports 2019, 9: 14200. PMID: 31578346, PMCID: PMC6775143, DOI: 10.1038/s41598-019-50271-4.Peer-Reviewed Original ResearchConceptsCompound Kushen InjectionExperimental biology approachesEffect of CKIPlant secondary metabolitesCytokine Signaling PathwaysCKI activityBiology approachReconstitution approachGene expressionCell cyclePhenotype dataSignaling pathwaysSecondary metabolitesBreast cancer cell linesCancer cell linesKushen InjectionSingle major compoundCell linesApoptosisLiquid chromatography fractionationCell viabilityHigh-performance liquid chromatography fractionationMajor compoundsComplex fashionCandidate mechanismEffect of Compound Kushen Injection, a Natural Compound Mixture, and Its Identified Chemical Components on Migration and Invasion of Colon, Brain, and Breast Cancer Cell Lines
Nourmohammadi S, Aung TN, Cui J, Pei JV, De Ieso ML, Harata-Lee Y, Qu Z, Adelson DL, Yool AJ. Effect of Compound Kushen Injection, a Natural Compound Mixture, and Its Identified Chemical Components on Migration and Invasion of Colon, Brain, and Breast Cancer Cell Lines. Frontiers In Oncology 2019, 9: 314. PMID: 31106149, PMCID: PMC6498862, DOI: 10.3389/fonc.2019.00314.Peer-Reviewed Original ResearchCompound Kushen InjectionCell linesFocal adhesion genesBreast cancer cell linesCancer cell linesCell imagingActin cytoskeletalHuman embryonic kidneyTranscriptomic analysisAdhesion genesMDA-MB-231 cellsCell cycleBreast cancerInvasion of colonKushen InjectionEmbryonic kidneyCell migrationExtracellular matrixTranswell invasionNatural compound mixturesCKI treatmentHEK-293Cancer migrationNon-cancerous controlsCancer metastasis