2024
Levels of Cytokines in Leptospirosis Patients with Different Serovars and rfb Locus
Senavirathna I, Jayasundara D, Warnasekara J, Kappagoda C, Agampodi S. Levels of Cytokines in Leptospirosis Patients with Different Serovars and rfb Locus. Journal Of Interferon & Cytokine Research 2024, 44: 80-93. PMID: 38377491, PMCID: PMC10880283, DOI: 10.1089/jir.2023.0091.Peer-Reviewed Original ResearchConceptsRfb locusGenome sequencePacBio RS II systemBacterial cell wallCulture-positive samplesLipopolysaccharide synthesisRapid AnnotationLeptospira borgpetersenii</i>,Genomic dataMicroscopic agglutination testSubsystem TechnologyLociCell wallSerovarsIsolated culturesSevere clinical manifestationsCytokine responsesLeptospira interrogans serovar autumnalisClinical manifestationsGenomeSamples of cultureSequenceRFBLevels of cytokinesSerum cytokine concentrations
2023
Global, regional, and country-level cost of leptospirosis due to loss of productivity in humans
Agampodi S, Gunarathna S, Lee J, Excler J. Global, regional, and country-level cost of leptospirosis due to loss of productivity in humans. PLOS Neglected Tropical Diseases 2023, 17: e0011291. PMID: 37616329, PMCID: PMC10482283, DOI: 10.1371/journal.pntd.0011291.Peer-Reviewed Original ResearchConceptsGross domestic productDisability-adjusted life yearsProductivity costsWorld Bank data repositoryLoss of productivityHealth expenditure dataEconomic burden estimatesHigher productivity costsDomestic productExpenditure dataInternational dollarsOverall economic burdenMonetary valueEconomic burdenAsia-Pacific regionIntangible costsLife yearsEconomic costsCost estimatesIncome countriesDiseases of povertyPartial estimatesCostProductivityCountriesComplete genome sequences of twelve strains of Leptospira interrogans isolated from humans in Sri Lanka
Senavirathna I, Jayasundara D, Warnasekara J, Matthias M, Vinetz J, Agampodi S. Complete genome sequences of twelve strains of Leptospira interrogans isolated from humans in Sri Lanka. Infection Genetics And Evolution 2023, 113: 105462. PMID: 37301334, DOI: 10.1016/j.meegid.2023.105462.Peer-Reviewed Original Research
2022
Culture-Independent Detection and Identification of Leptospira Serovars
Matthias M, Lubar A, Acharige S, Chaiboonma K, Pilau N, Marroquin A, Jayasundara D, Agampodi S, Vinetz J. Culture-Independent Detection and Identification of Leptospira Serovars. Microbiology Spectrum 2022, 10: e02475-22. PMID: 36445143, PMCID: PMC9769591, DOI: 10.1128/spectrum.02475-22.Peer-Reviewed Original ResearchConceptsPublic health importanceProspective studyFatal diseaseHealth importanceOutbreak investigationSequence typingDog urine samplesCulture-independent assaysEnvironmental surveillance studyMultilocus sequence typingPulsed-field gel electrophoresisSurveillance studyGroup IIHuman leptospirosisPrognostic testSpecific real-time qPCRClinical settingGroup ILeptospira serovarsReal-time qPCRDiagnostic testsSerum samplesUrine samplesDiagnostic specificityLeptospirosisSARIMA and ARDL models for predicting leptospirosis in Anuradhapura district Sri Lanka
Warnasekara J, Agampodi S, Nr A. SARIMA and ARDL models for predicting leptospirosis in Anuradhapura district Sri Lanka. PLOS ONE 2022, 17: e0275447. PMID: 36227833, PMCID: PMC9562162, DOI: 10.1371/journal.pone.0275447.Peer-Reviewed Original ResearchDiagnostic method-based underestimation of leptospirosis in clinical and research settings; an experience from a large prospective study in a high endemic setting
Warnasekara J, Srimantha S, Kappagoda C, Jayasundara D, Senevirathna I, Matthias M, Agampodi S, Vinetz J. Diagnostic method-based underestimation of leptospirosis in clinical and research settings; an experience from a large prospective study in a high endemic setting. PLOS Neglected Tropical Diseases 2022, 16: e0010331. PMID: 35377883, PMCID: PMC9009773, DOI: 10.1371/journal.pntd.0010331.Peer-Reviewed Original ResearchMeSH KeywordsAgglutination TestsHumansLeptospiraLeptospirosisProspective StudiesRetrospective StudiesConceptsMicroscopic agglutination testProspective hospital-based studyLeptospirosis casesQuantitative PCRHigh endemic settingHospital-based studyClinical case definitionLarge prospective studiesDisease burden estimatesPublic health burdenStandard diagnostic testMonths of observationUndifferentiated feverAcute leptospirosisProspective studyCase definitionEndemic settingsHealth burdenTimely diagnosisBurden estimatesDiagnostic valueSignificant human mortalityAgglutination testStudy settingDiagnostic testsNeglecting the neglected during the COVID-19 pandemic: the case of leptospirosis in Sri Lanka
Warnasekara J, Agampodi S. Neglecting the neglected during the COVID-19 pandemic: the case of leptospirosis in Sri Lanka. Epidemiology And Health 2022, 44: e2022015. PMID: 35038829, PMCID: PMC9117097, DOI: 10.4178/epih.e2022015.Peer-Reviewed Original ResearchConceptsCOVID-19 pandemicCoronavirus disease 2019 (COVID-19) pandemicPublic health surveillance systemsCases of leptospirosisControl of leptospirosisDisease 2019 pandemicHealth surveillance systemsGlobal health systemLarge outbreakInfectious diseasesHealth systemLeptospirosisTransmission dynamicsSurveillance systemPandemicSurveillanceSri LankaDisease
2021
Clinical spectrum of endemic leptospirosis in relation to cytokine response
Dahanayaka NJ, Agampodi SB, Seneviratna I, Warnasekara J, Rajapakse R, Ranathunga K, Matthias M, Vinetz JM. Clinical spectrum of endemic leptospirosis in relation to cytokine response. PLOS ONE 2021, 16: e0261025. PMID: 34879100, PMCID: PMC8654203, DOI: 10.1371/journal.pone.0261025.Peer-Reviewed Original ResearchConceptsCytokine responsesLeptospirosis patientsClinical spectrumHigher IL-13 levelsHigher IL-5Acute kidney injuryHigher serum creatinineIL-13 levelsSerum cytokine levelsIL-6 levelsUniversity Teaching HospitalMultiplex cytokine kitKidney injuryLung involvementCytokine levelsSerum creatinineAcute phaseCytokine kitIL-1βAcute samplesConvalescence periodIL-8IL-5Prognostic informationEndemic settingsEstimating the burden of leptospirosis: global lessons from Sri Lanka
Warnasekara J, Srimantha SM, Agampodi SB. Estimating the burden of leptospirosis: global lessons from Sri Lanka. BMJ Global Health 2021, 6: e006946. PMID: 34706880, PMCID: PMC8552134, DOI: 10.1136/bmjgh-2021-006946.Peer-Reviewed Original ResearchOptimizing the microscopic agglutination test (MAT) panel for the diagnosis of Leptospirosis in a low resource, hyper-endemic setting with varied microgeographic variation in reactivity
Jayasundara D, Gamage C, Senavirathna I, Warnasekara J, Matthias MA, Vinetz J, Agampodi S. Optimizing the microscopic agglutination test (MAT) panel for the diagnosis of Leptospirosis in a low resource, hyper-endemic setting with varied microgeographic variation in reactivity. PLOS Neglected Tropical Diseases 2021, 15: e0009565. PMID: 34197457, PMCID: PMC8279374, DOI: 10.1371/journal.pntd.0009565.Peer-Reviewed Original ResearchTime series models for prediction of leptospirosis in different climate zones in Sri Lanka
Warnasekara J, Agampodi S, R. R. Time series models for prediction of leptospirosis in different climate zones in Sri Lanka. PLOS ONE 2021, 16: e0248032. PMID: 33989292, PMCID: PMC8121312, DOI: 10.1371/journal.pone.0248032.Peer-Reviewed Original ResearchImproving the leptospirosis disease burden assessment by including ambulatory patients from outpatient departments: a cross-sectional study
Warnasekara J, Aberathna P, Nanayakkara G, Vinetz J, Agampodi S. Improving the leptospirosis disease burden assessment by including ambulatory patients from outpatient departments: a cross-sectional study. F1000Research 2021, 9: 1129. PMID: 34123371, PMCID: PMC8145224, DOI: 10.12688/f1000research.26202.2.Peer-Reviewed Original ResearchConceptsOutpatient departmentOPD patientsAmbulatory patientsDisease burdenPrimary care centersProvincial General HospitalCases of leptospirosisCross-sectional studyDisease burden assessmentMicroscopic agglutination testPublic health implicationsQuantitative polymerase chain reactionOPD settingRegional Public HospitalOPD visitsLeptospirosis patientsFever patientsPatient numbersHospital inpatientsPolymerase chain reactionCare centerGeneral HospitalBurden assessmentPatientsSame periodNext-Generation Sequencing Analysis of Pathogenic Leptospira: A Way Forward for Understanding Infectious Disease Dynamics in Low/Middle-Income, Disease-Endemic Settings.
Agampodi SB, Vinetz JM. Next-Generation Sequencing Analysis of Pathogenic Leptospira: A Way Forward for Understanding Infectious Disease Dynamics in Low/Middle-Income, Disease-Endemic Settings. American Journal Of Tropical Medicine And Hygiene 2021, 104: 1625-1627. PMID: 33755589, PMCID: PMC8103479, DOI: 10.4269/ajtmh.20-1518.Peer-Reviewed Original Research12 Novel clonal groups of Leptospira infecting humans in multiple contrasting epidemiological contexts in Sri Lanka
Jayasundara D, Senavirathna I, Warnasekara J, Gamage C, Siribaddana S, Kularatne SAM, Matthias M, Mariet JF, Picardeau M, Agampodi S, Vinetz J. 12 Novel clonal groups of Leptospira infecting humans in multiple contrasting epidemiological contexts in Sri Lanka. PLOS Neglected Tropical Diseases 2021, 15: e0009272. PMID: 33735202, PMCID: PMC8009393, DOI: 10.1371/journal.pntd.0009272.Peer-Reviewed Original ResearchConceptsClonal groupsAcute kidney injuryL. interrogansMajor clinical challengeGlobal disease threatsLeptospira speciesL. interrogans infectionKidney injuryLiver impairmentCardiovascular involvementL. borgpeterseniiClinical presentationProspective studyClinical severityClinical challengePathogenic Leptospira speciesAnnual epidemicsUbiquitous zoonotic diseaseNew serogroupEpidemiological contextZoonotic diseaseCulture isolationLeptospiral culturesL. weiliiLeptospira diversity
2020
Cytokine response in human leptospirosis with different clinical outcomes: a systematic review
Senavirathna I, Rathish D, Agampodi S. Cytokine response in human leptospirosis with different clinical outcomes: a systematic review. BMC Infectious Diseases 2020, 20: 268. PMID: 32264832, PMCID: PMC7137275, DOI: 10.1186/s12879-020-04986-9.Peer-Reviewed Original ResearchConceptsDifferent clinical outcomesCross-sectional studyCytokine responsesIL-10Clinical outcomesSystematic reviewDisease severityHealth Quality Assessment ToolComparative cross-sectional studyTNF-α levelsMethodsA systematic reviewMeasurement of cytokinesEnzyme-linked immunosorbent assayMicroscopic agglutination testPublic health professionalsQuality assessment toolMild leptospirosisCytokine stormElevated cytokinesObservational cohortLeptospirosis patientsProspective studyIL-1βIL-6IL-8
2019
Study protocol: characterising the clinical, epidemiological and aetiological aspects of leptospirosis in Sri Lanka: a hospital based clinico-epidemiological study
Agampodi S, Warnasekara J, Jayasundara D, Senawirathna I, Gamage C, Kularatne S, Siribaddana S, Maththias M, Vinetz J. Study protocol: characterising the clinical, epidemiological and aetiological aspects of leptospirosis in Sri Lanka: a hospital based clinico-epidemiological study. BMJ Open 2019, 9: e027850. PMID: 31511281, PMCID: PMC6738675, DOI: 10.1136/bmjopen-2018-027850.Peer-Reviewed Original ResearchConceptsEthics Review CommitteeMultilocus sequence typingOutpatient departmentClinico-epidemiological studyMulticentre prospective studyDay of admissionMicroscopic agglutination testPresence of diseaseHospital stayFebrile patientsMedical wardsProspective studySri Lankan isolatesRisk factorsVenous bloodDisease progressionEthical clearanceHigh incidenceAetiological aspectsPatientsAgglutination testMolecular epidemiologyPatient contextBedside inoculationLeptospirosisA Case–Control Study of Environmental and Occupational Risks of Leptospirosis in Sri Lanka
Schønning MH, Phelps MD, Warnasekara J, Agampodi SB, Furu P. A Case–Control Study of Environmental and Occupational Risks of Leptospirosis in Sri Lanka. EcoHealth 2019, 16: 534-543. PMID: 31664587, DOI: 10.1007/s10393-019-01448-w.Peer-Reviewed Original ResearchConceptsPaddy workersOdds ratioSignificant higher riskCase-control studyHigher odds ratioPublic health interventionsTransmission of leptospirosisRisk factorsOccupational exposureProtective effectHigh riskHigh incidenceRat exposureHuman leptospirosisViral infectionHealth interventionsZoonotic infectionOccupational riskLeptospirosisLeptospirosis transmissionStudy sampleOccupational groupsInfectionStructured interviewsHousehold animalsSequel and therapeutic modalities of leptospirosis associated severe pulmonary haemorrhagic syndrome (SPHS); a Sri Lankan experience
Herath N, Uluwattage W, Weliwitiya T, Karunanayake L, Lekamwasam S, Ratnatunga N, Karunanayake P, Wickramasinghe S, Patabendi S, Senaviratne S, Agampodi S. Sequel and therapeutic modalities of leptospirosis associated severe pulmonary haemorrhagic syndrome (SPHS); a Sri Lankan experience. BMC Infectious Diseases 2019, 19: 451. PMID: 31113404, PMCID: PMC6530063, DOI: 10.1186/s12879-019-4094-0.Peer-Reviewed Original ResearchConceptsTeaching Hospital KarapitiyaTherapeutic plasma exchangeAcute kidney injuryMain tertiary care centerSevere pulmonary hemorrhagic syndromeTertiary care centerDay of admissionGroup of patientsHigh case fatalityCase fatality rateCases of leptospirosisDifferent therapeutic approachesAlveolar shadowsHospital KarapitiyaHemoglobin dropKidney injuryArterial hypoxemiaCase fatalityPlasma exchangePatient presentingTreatment modalitiesCare centerHemorrhagic syndromeChest radiographsClinical diseaseAn outbreak of leptospirosis with predominant cardiac involvement: a case series
Jayathilaka PGNS, Mendis ASV, Perera MHMTS, Damsiri HMT, Gunaratne AVC, Agampodi SB. An outbreak of leptospirosis with predominant cardiac involvement: a case series. BMC Infectious Diseases 2019, 19: 265. PMID: 30885170, PMCID: PMC6423826, DOI: 10.1186/s12879-019-3905-7.Peer-Reviewed Original ResearchConceptsColombo North Teaching HospitalCardiac involvementMulti-organ dysfunctionCase seriesSevere leptospirosisTeaching hospitalDynamic T-wave changesInotropic/vasopressor supportNon-specific clinical findingsEvidence of myocarditisPredominant cardiac involvementRapid atrial fibrillationSingle medical wardAcute kidney injuryMean arterial pressureStudy of patientsCourse of illnessT-wave changesCase presentationWe reportOutbreak of leptospirosisPossible leptospirosisResidual complicationsVasopressor supportKidney injuryCommon complicationEstimating the burden of leptospirosis in Sri Lanka; a systematic review
Warnasekara J, Koralegedara I, Agampodi S. Estimating the burden of leptospirosis in Sri Lanka; a systematic review. BMC Infectious Diseases 2019, 19: 119. PMID: 30727968, PMCID: PMC6364467, DOI: 10.1186/s12879-018-3655-y.Peer-Reviewed Original ResearchConceptsSystematic reviewPooled case fatality ratioCumulative annual incidenceMortality of leptospirosisBurden of leptospirosisConclusionOur systematic reviewCase fatality ratioCurrent Contents ConnectRelevant full textsRoutine surveillance dataFull-text reviewBIOSIS Citation IndexMethodA systematic reviewGoogle Scholar databasesCommon organAnnual incidenceHospitalization ratesTrue burdenDisease burdenBurden estimatesBIOSIS PreviewsCitation IndexKCI-Korean Journal DatabaseAnnual caseloadFatality ratio