2023
Complete genome sequences of twelve strains of Leptospira interrogans isolated from humans in Sri Lanka
Senavirathna I, Jayasundara D, Warnasekara J, Matthias M, Vinetz J, Agampodi S. Complete genome sequences of twelve strains of Leptospira interrogans isolated from humans in Sri Lanka. Infection Genetics And Evolution 2023, 113: 105462. PMID: 37301334, DOI: 10.1016/j.meegid.2023.105462.Peer-Reviewed Original Research
2022
Culture-Independent Detection and Identification of Leptospira Serovars
Matthias M, Lubar A, Acharige S, Chaiboonma K, Pilau N, Marroquin A, Jayasundara D, Agampodi S, Vinetz J. Culture-Independent Detection and Identification of Leptospira Serovars. Microbiology Spectrum 2022, 10: e02475-22. PMID: 36445143, PMCID: PMC9769591, DOI: 10.1128/spectrum.02475-22.Peer-Reviewed Original ResearchConceptsPublic health importanceProspective studyFatal diseaseHealth importanceOutbreak investigationSequence typingDog urine samplesCulture-independent assaysEnvironmental surveillance studyMultilocus sequence typingPulsed-field gel electrophoresisSurveillance studyGroup IIHuman leptospirosisPrognostic testSpecific real-time qPCRClinical settingGroup ILeptospira serovarsReal-time qPCRDiagnostic testsSerum samplesUrine samplesDiagnostic specificityLeptospirosisDiagnostic method-based underestimation of leptospirosis in clinical and research settings; an experience from a large prospective study in a high endemic setting
Warnasekara J, Srimantha S, Kappagoda C, Jayasundara D, Senevirathna I, Matthias M, Agampodi S, Vinetz J. Diagnostic method-based underestimation of leptospirosis in clinical and research settings; an experience from a large prospective study in a high endemic setting. PLOS Neglected Tropical Diseases 2022, 16: e0010331. PMID: 35377883, PMCID: PMC9009773, DOI: 10.1371/journal.pntd.0010331.Peer-Reviewed Original ResearchMeSH KeywordsAgglutination TestsHumansLeptospiraLeptospirosisProspective StudiesRetrospective StudiesConceptsMicroscopic agglutination testProspective hospital-based studyLeptospirosis casesQuantitative PCRHigh endemic settingHospital-based studyClinical case definitionLarge prospective studiesDisease burden estimatesPublic health burdenStandard diagnostic testMonths of observationUndifferentiated feverAcute leptospirosisProspective studyCase definitionEndemic settingsHealth burdenTimely diagnosisBurden estimatesDiagnostic valueSignificant human mortalityAgglutination testStudy settingDiagnostic testsThe variable presence of Leptospira in the environment; an epidemiological explanation based on serial analysis of water samples
Warnasekara J, Srimantha S, Senavirathna I, Kappagoda C, Farika N, Nawinna A, Agampodi S. The variable presence of Leptospira in the environment; an epidemiological explanation based on serial analysis of water samples. PLOS ONE 2022, 17: e0263719. PMID: 35167605, PMCID: PMC8846531, DOI: 10.1371/journal.pone.0263719.Peer-Reviewed Original ResearchMeSH KeywordsDNA, BacterialLeptospiraPhylogenySoil MicrobiologySri LankaWater MicrobiologyWater WellsConceptsSoil sample analysisSame water sourceSoil samplesWater samplingDifferent climate zonesWet zoneWater sitesWater sourcesWater sample analysisAnimal urineDry zoneClimate zonesNatural environmentVariable abundanceWater samplesMore replicatesSoilOpen wellsSri LankaPresence of LeptospiraMain roadAbundanceAmplicon analysisWaterGreater distances
2021
Clinical spectrum of endemic leptospirosis in relation to cytokine response
Dahanayaka NJ, Agampodi SB, Seneviratna I, Warnasekara J, Rajapakse R, Ranathunga K, Matthias M, Vinetz JM. Clinical spectrum of endemic leptospirosis in relation to cytokine response. PLOS ONE 2021, 16: e0261025. PMID: 34879100, PMCID: PMC8654203, DOI: 10.1371/journal.pone.0261025.Peer-Reviewed Original ResearchConceptsCytokine responsesLeptospirosis patientsClinical spectrumHigher IL-13 levelsHigher IL-5Acute kidney injuryHigher serum creatinineIL-13 levelsSerum cytokine levelsIL-6 levelsUniversity Teaching HospitalMultiplex cytokine kitKidney injuryLung involvementCytokine levelsSerum creatinineAcute phaseCytokine kitIL-1βAcute samplesConvalescence periodIL-8IL-5Prognostic informationEndemic settingsOptimizing the microscopic agglutination test (MAT) panel for the diagnosis of Leptospirosis in a low resource, hyper-endemic setting with varied microgeographic variation in reactivity
Jayasundara D, Gamage C, Senavirathna I, Warnasekara J, Matthias MA, Vinetz J, Agampodi S. Optimizing the microscopic agglutination test (MAT) panel for the diagnosis of Leptospirosis in a low resource, hyper-endemic setting with varied microgeographic variation in reactivity. PLOS Neglected Tropical Diseases 2021, 15: e0009565. PMID: 34197457, PMCID: PMC8279374, DOI: 10.1371/journal.pntd.0009565.Peer-Reviewed Original ResearchNext-Generation Sequencing Analysis of Pathogenic Leptospira: A Way Forward for Understanding Infectious Disease Dynamics in Low/Middle-Income, Disease-Endemic Settings.
Agampodi SB, Vinetz JM. Next-Generation Sequencing Analysis of Pathogenic Leptospira: A Way Forward for Understanding Infectious Disease Dynamics in Low/Middle-Income, Disease-Endemic Settings. American Journal Of Tropical Medicine And Hygiene 2021, 104: 1625-1627. PMID: 33755589, PMCID: PMC8103479, DOI: 10.4269/ajtmh.20-1518.Peer-Reviewed Original Research12 Novel clonal groups of Leptospira infecting humans in multiple contrasting epidemiological contexts in Sri Lanka
Jayasundara D, Senavirathna I, Warnasekara J, Gamage C, Siribaddana S, Kularatne SAM, Matthias M, Mariet JF, Picardeau M, Agampodi S, Vinetz J. 12 Novel clonal groups of Leptospira infecting humans in multiple contrasting epidemiological contexts in Sri Lanka. PLOS Neglected Tropical Diseases 2021, 15: e0009272. PMID: 33735202, PMCID: PMC8009393, DOI: 10.1371/journal.pntd.0009272.Peer-Reviewed Original ResearchConceptsClonal groupsAcute kidney injuryL. interrogansMajor clinical challengeGlobal disease threatsLeptospira speciesL. interrogans infectionKidney injuryLiver impairmentCardiovascular involvementL. borgpeterseniiClinical presentationProspective studyClinical severityClinical challengePathogenic Leptospira speciesAnnual epidemicsUbiquitous zoonotic diseaseNew serogroupEpidemiological contextZoonotic diseaseCulture isolationLeptospiral culturesL. weiliiLeptospira diversity
2019
Study protocol: characterising the clinical, epidemiological and aetiological aspects of leptospirosis in Sri Lanka: a hospital based clinico-epidemiological study
Agampodi S, Warnasekara J, Jayasundara D, Senawirathna I, Gamage C, Kularatne S, Siribaddana S, Maththias M, Vinetz J. Study protocol: characterising the clinical, epidemiological and aetiological aspects of leptospirosis in Sri Lanka: a hospital based clinico-epidemiological study. BMJ Open 2019, 9: e027850. PMID: 31511281, PMCID: PMC6738675, DOI: 10.1136/bmjopen-2018-027850.Peer-Reviewed Original ResearchConceptsEthics Review CommitteeMultilocus sequence typingOutpatient departmentClinico-epidemiological studyMulticentre prospective studyDay of admissionMicroscopic agglutination testPresence of diseaseHospital stayFebrile patientsMedical wardsProspective studySri Lankan isolatesRisk factorsVenous bloodDisease progressionEthical clearanceHigh incidenceAetiological aspectsPatientsAgglutination testMolecular epidemiologyPatient contextBedside inoculationLeptospirosisA Case–Control Study of Environmental and Occupational Risks of Leptospirosis in Sri Lanka
Schønning MH, Phelps MD, Warnasekara J, Agampodi SB, Furu P. A Case–Control Study of Environmental and Occupational Risks of Leptospirosis in Sri Lanka. EcoHealth 2019, 16: 534-543. PMID: 31664587, DOI: 10.1007/s10393-019-01448-w.Peer-Reviewed Original ResearchConceptsPaddy workersOdds ratioSignificant higher riskCase-control studyHigher odds ratioPublic health interventionsTransmission of leptospirosisRisk factorsOccupational exposureProtective effectHigh riskHigh incidenceRat exposureHuman leptospirosisViral infectionHealth interventionsZoonotic infectionOccupational riskLeptospirosisLeptospirosis transmissionStudy sampleOccupational groupsInfectionStructured interviewsHousehold animals
2014
Regional Differences of Leptospirosis in Sri Lanka: Observations from a Flood-Associated Outbreak in 2011
Agampodi SB, Dahanayaka NJ, Bandaranayaka AK, Perera M, Priyankara S, Weerawansa P, Matthias MA, Vinetz JM. Regional Differences of Leptospirosis in Sri Lanka: Observations from a Flood-Associated Outbreak in 2011. PLOS Neglected Tropical Diseases 2014, 8: e2626. PMID: 24454971, PMCID: PMC3894175, DOI: 10.1371/journal.pntd.0002626.Peer-Reviewed Original ResearchConceptsOutbreak of leptospirosisClinical presentationAcute renal failureDifferent clinical presentationsCross-sectional studyMedian bacterial loadPublic health interventionsPublic health relevanceLeptospira interrogans serovar laiRenal failureSevere thrombocytopeniaInfectious disease epidemicsDifferent Leptospira speciesEtiological causesImportant causeHealth interventionsHealth relevanceLeptospirosisTropical diseasesBacterial loadLeptospira kirschneriLeptospirosis outbreaksSerovar laiLeptospira speciesL. kirschneri
2012
Utility and Limitations of Direct Multi-Locus Sequence Typing on qPCR-Positive Blood to Determine Infecting Leptospira Strain
Agampodi SB, Moreno AC, Vinetz JM, Matthias MA. Utility and Limitations of Direct Multi-Locus Sequence Typing on qPCR-Positive Blood to Determine Infecting Leptospira Strain. American Journal Of Tropical Medicine And Hygiene 2012, 88: 184-185. PMID: 23208890, PMCID: PMC3541733, DOI: 10.4269/ajtmh.2012.12-0526.Peer-Reviewed Original ResearchConceptsPolymerase chain reaction-positive samplesBacterial loadLeptospira strainsMLST profilesAcute illnessBlood samplesHuman leptospirosisComplete MLST profilesCulture isolationMulti-locus sequenceHuman bloodSequence typesBloodCentral Sri LankaInterpretable dataMolecular characterizationIllnessLeptospirosisST44Utility of Quantitative Polymerase Chain Reaction in Leptospirosis Diagnosis: Association of Level of Leptospiremia and Clinical Manifestations in Sri Lanka
Agampodi SB, Matthias MA, Moreno AC, Vinetz JM. Utility of Quantitative Polymerase Chain Reaction in Leptospirosis Diagnosis: Association of Level of Leptospiremia and Clinical Manifestations in Sri Lanka. Clinical Infectious Diseases 2012, 54: 1249-1255. PMID: 22354922, PMCID: PMC3404689, DOI: 10.1093/cid/cis035.Peer-Reviewed Original ResearchConceptsQuantitative polymerase chain reactionClinical manifestationsPolymerase chain reactionBlood samplesChain reactionWhole bloodOnset of symptomsSensitivity of qPCRAssociation of levelsSerum/bloodMicroscopic agglutination test panelValid diagnostic testPatient blood samplesOptimal sample typeFailure patientsRenal failureWhole blood samplesPatient populationTimely diagnosisLeptospiral loadEndemic areasDay 2Day 15LeptospiremiaDiagnostic tests
2008
A preliminary study on prevalent serovars of leptospirosis among patients admitted to teaching hospital, Kandy, Sri Lanka
Agampodi S, Thevanesam V, Wimalarathna H, Senarathna T, Wijedasa M. A preliminary study on prevalent serovars of leptospirosis among patients admitted to teaching hospital, Kandy, Sri Lanka. Indian Journal Of Medical Microbiology 2008, 26: 405-406. PMID: 18974510, DOI: 10.4103/0255-0857.43557.Peer-Reviewed Original Research