1995
Studies of Point Mutants Define Three Essential Paired Nucleotides in the Domain 5 Substructure of a Group II Intron
Boulanger S, Belcher S, Schmidt U, Dib-Hajj S, Schmidt T, Perlman P. Studies of Point Mutants Define Three Essential Paired Nucleotides in the Domain 5 Substructure of a Group II Intron. Molecular And Cellular Biology 1995, 15: 4479-4488. PMID: 7623838, PMCID: PMC230687, DOI: 10.1128/mcb.15.8.4479.Peer-Reviewed Original ResearchConceptsGroup II intronsU6 small nuclear RNADominant nuclear suppressorBase pairsSaccharomyces cerevisiae mitochondriaPoint mutationsSecond-site suppressorsSmall nuclear RNAYeast U2Nuclear suppressorCoxI geneCerevisiae mitochondriaRespiratory growthVivo splicingBiolistic transformationNuclear RNAHelix 1C base pairsSplicingIntronsDomain 5MutantsMutationsNucleotidesSuppressor
1992
Group II introns deleted for multiple substructures retain self-splicing activity.
Koch J, Boulanger S, Dib-Hajj S, Hebbar S, Perlman P. Group II introns deleted for multiple substructures retain self-splicing activity. Molecular And Cellular Biology 1992, 12: 1950-1958. PMID: 1569932, PMCID: PMC364365, DOI: 10.1128/mcb.12.5.1950.Peer-Reviewed Original ResearchConceptsGroup II intronsSplice site selectionSelf-splicing activitySplice junctionsDomain 2Domain 5Domain 1Essential catalytic coreYeast mitochondrial DNASecondary structure elementsCoxI geneSelf-splicing reactionMitochondrial DNAPrecursor RNAMutant intronsCatalytic coreIntron domainsTriple deletionIntronsTrans assaySingle deletionSecondary structureDomain 6DeletionStructure elements