1995
A UV-induced, Mg(2+)-dependent crosslink traps an active form of domain 3 of a self-splicing group II intron.
Podar M, Dib-Hajj S, Perlman P. A UV-induced, Mg(2+)-dependent crosslink traps an active form of domain 3 of a self-splicing group II intron. RNA 1995, 1: 828-40. PMID: 7493328, PMCID: PMC1369323.Peer-Reviewed Original ResearchStudies of Point Mutants Define Three Essential Paired Nucleotides in the Domain 5 Substructure of a Group II Intron
Boulanger S, Belcher S, Schmidt U, Dib-Hajj S, Schmidt T, Perlman P. Studies of Point Mutants Define Three Essential Paired Nucleotides in the Domain 5 Substructure of a Group II Intron. Molecular And Cellular Biology 1995, 15: 4479-4488. PMID: 7623838, PMCID: PMC230687, DOI: 10.1128/mcb.15.8.4479.Peer-Reviewed Original ResearchConceptsGroup II intronsU6 small nuclear RNADominant nuclear suppressorBase pairsSaccharomyces cerevisiae mitochondriaPoint mutationsSecond-site suppressorsSmall nuclear RNAYeast U2Nuclear suppressorCoxI geneCerevisiae mitochondriaRespiratory growthVivo splicingBiolistic transformationNuclear RNAHelix 1C base pairsSplicingIntronsDomain 5MutantsMutationsNucleotidesSuppressor
1993
Domain 5 interacts with domain 6 and influences the second transesterification reaction of group II intron self-splicing
Dib-Hajj S, Boulanger S, Hebbar S, Peebles C, Franzen J, Perlman P. Domain 5 interacts with domain 6 and influences the second transesterification reaction of group II intron self-splicing. Nucleic Acids Research 1993, 21: 1797-1804. PMID: 8493099, PMCID: PMC309417, DOI: 10.1093/nar/21.8.1797.Peer-Reviewed Original ResearchConceptsSplice site useSplice site utilizationSplice siteDomain 5Yeast mitochondrial DNAGroup II intronsSecond transesterification reactionSubstrate transcriptsCoxI geneSplicing occursMitochondrial DNARNA moleculesDomain 6RNAExonsD5 RNASite utilizationTranscriptsKey roleIntronsBranchingSite residentsSite useGenesSites
1992
Group II introns deleted for multiple substructures retain self-splicing activity.
Koch J, Boulanger S, Dib-Hajj S, Hebbar S, Perlman P. Group II introns deleted for multiple substructures retain self-splicing activity. Molecular And Cellular Biology 1992, 12: 1950-1958. PMID: 1569932, PMCID: PMC364365, DOI: 10.1128/mcb.12.5.1950.Peer-Reviewed Original ResearchConceptsGroup II intronsSplice site selectionSelf-splicing activitySplice junctionsDomain 2Domain 5Domain 1Essential catalytic coreYeast mitochondrial DNASecondary structure elementsCoxI geneSelf-splicing reactionMitochondrial DNAPrecursor RNAMutant intronsCatalytic coreIntron domainsTriple deletionIntronsTrans assaySingle deletionSecondary structureDomain 6DeletionStructure elements