2013
Wound-healing growth factor, basic FGF, induces Erk1/2-dependent mechanical hyperalgesia
Andres C, Hasenauer J, Ahn H, Joseph EK, Isensee J, Theis FJ, Allgöwer F, Levine JD, Dib-Hajj S, Waxman SG, Hucho T. Wound-healing growth factor, basic FGF, induces Erk1/2-dependent mechanical hyperalgesia. Pain 2013, 154: 2216-2226. PMID: 23867734, DOI: 10.1016/j.pain.2013.07.005.Peer-Reviewed Original ResearchConceptsWound-healing factorsBasic fibroblast growth factorDorsal root gangliaDRG neuronsNociceptive neuronsGrowth factorMechanical hyperalgesiaPain sensitizationGlial cell line-derived neurotrophic factorRat dorsal root gangliaLine-derived neurotrophic factorSingle-cell electrophysiological recordingsLumbar DRG neuronsTranscription-polymerase chain reactionNerve growth factorWound healing growth factorsFibroblast growth factorTime-dependent mannerNeurotrophic factorRoot gangliaPolymerase chain reactionIntradermal injectionNav1.8 channelsBFGF treatmentElectrophysiological recordings
1998
Glial cells have heart: rH1 Na+ channel mRNA and protein in spinal cord astrocytes
Black JA, Dib‐Hajj S, Cohen S, Hinson AW, Waxman SG. Glial cells have heart: rH1 Na+ channel mRNA and protein in spinal cord astrocytes. Glia 1998, 23: 200-208. PMID: 9633805, DOI: 10.1002/(sici)1098-1136(199807)23:3<200::aid-glia3>3.0.co;2-8.Peer-Reviewed Original ResearchConceptsTTX-resistant currentStellate astrocytesSpinal cordCultured spinal cord astrocytesVoltage-sensitive sodium currentsChannel mRNASodium currentDorsal root ganglion neuronsSodium channel immunoreactivityRT-PCRSpinal cord astrocytesTTX-sensitive currentsIntact spinal cordSpinal cord culturesReverse transcription-polymerase chain reactionSodium channel mRNASodium channel expressionTranscription-polymerase chain reactionTTX-resistant channelsPolyclonal antibodiesChannel immunoreactivityP7 ratsGanglion neuronsPolymerase chain reactionChannel antibodies
1997
TTX-Sensitive and -Resistant Na+ Currents, and mRNA for the TTX-Resistant rH1 Channel, Are Expressed in B104 Neuroblastoma Cells
Gu X, Dib-Hajj S, Rizzo M, Waxman S. TTX-Sensitive and -Resistant Na+ Currents, and mRNA for the TTX-Resistant rH1 Channel, Are Expressed in B104 Neuroblastoma Cells. Journal Of Neurophysiology 1997, 77: 236-246. PMID: 9120565, DOI: 10.1152/jn.1997.77.1.236.Peer-Reviewed Original ResearchConceptsB104 neuroblastoma cellsTTX-resistant channelsB104 cellsNeuroblastoma cellsWhole-cell patch-clamp methodAbsence of TTXTTX-resistant currentTTX-sensitive currentsPresence of TTXPA/pFTranscription-polymerase chain reactionLong QT syndromeCell linesSteady-state inactivationNeuroblastoma cell linesAlpha-subunit mRNAPatch-clamp methodTTX-sensitiveHalf-maximal inhibitionInactivation time constantsChannel mRNATTXMembrane excitabilitySubunit mRNAsRT-PCR