2002
A novel family of anticoagulants from the saliva of Ixodes scapularis
Narasimhan S, Koski RA, Beaulieu B, Anderson JF, Ramamoorthi N, Kantor F, Cappello M, Fikrig E. A novel family of anticoagulants from the saliva of Ixodes scapularis. Insect Molecular Biology 2002, 11: 641-650. PMID: 12421422, DOI: 10.1046/j.1365-2583.2002.00375.x.Peer-Reviewed Original ResearchConceptsN-terminal amino acid sequenceSalivary gland cDNA libraryAmino acid sequenceN-terminal sequenceCDNA libraryAcid sequenceAnticoagulant proteinsIxodes scapularisMolecular approachesTerminal sequenceIntrinsic pathwayEscherichia coliSpecific inhibitorNovel familyProteinSalp14Different functionsTick salivaSequenceBiological activityDose-dependent mannerProtease inhibitorsReversed-phase HPLCParaloguesScapularis
1994
Gene for an extracellular matrix receptor protein from Pneumocystis carinii.
Narasimhan S, Armstrong M, Rhee K, Edman J, Richards F, Spicer E. Gene for an extracellular matrix receptor protein from Pneumocystis carinii. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 7440-7444. PMID: 7519777, PMCID: PMC44416, DOI: 10.1073/pnas.91.16.7440.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceCloning, MolecularDNA, ComplementaryExtracellular Matrix ProteinsFibronectinsGenes, FungalLamininMolecular Sequence DataNeoplasm ProteinsPneumocystisReceptors, Cell SurfaceReceptors, LamininRecombinant ProteinsRNASequence Homology, Amino AcidTranscription, GeneticConceptsReceptor proteinCDNA clonesCDNA sequenceECM receptorsC-terminal thirdFull-length cloneBlot analysisSouthern blot analysisExtracellular matrix proteinsNorthern blot analysisReceptor cDNA clonesMembrane associationCDNA libraryPC geneSequence homologyGenomic DNAN-terminusSequence analysisMatrix proteinsN-terminalReceptor cDNA sequenceHost cellsPredominant mRNAMolecular massAmino acids