2023
Croquemort elicits activation of the immune deficiency pathway in ticks
O’Neal A, Singh N, Rolandelli A, Laukaitis H, Wang X, Shaw D, Young B, Narasimhan S, Dutta S, Snyder G, Samaddar S, Marnin L, Butler L, Mendes M, Paz F, Valencia L, Sundberg E, Fikrig E, Pal U, Weber D, Pedra J. Croquemort elicits activation of the immune deficiency pathway in ticks. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2208673120. PMID: 37155900, PMCID: PMC10193931, DOI: 10.1073/pnas.2208673120.Peer-Reviewed Original ResearchConceptsImmune deficiency (IMD) pathwayIMD pathwayNon-insect arthropodsPeptidoglycan recognition proteinsJun N-terminal kinaseN-terminal kinaseArthropod immunityMembrane localizationRecognition proteinsLyme disease spirocheteEcdysteroid synthesisMicrobial moietiesDistinct mechanismsProteinArthropodsPathwayHost defenseElicit activationCroquemortPancrustaceaHomologInsectsActivationCrustaceansKinase
2011
A Tick Mannose-Binding Lectin Inhibitor Interferes with the Vertebrate Complement Cascade to Enhance Transmission of the Lyme Disease Agent
Schuijt TJ, Coumou J, Narasimhan S, Dai J, DePonte K, Wouters D, Brouwer M, Oei A, Roelofs JJ, van Dam AP, van der Poll T, Veer C, Hovius JW, Fikrig E. A Tick Mannose-Binding Lectin Inhibitor Interferes with the Vertebrate Complement Cascade to Enhance Transmission of the Lyme Disease Agent. Cell Host & Microbe 2011, 10: 136-146. PMID: 21843870, PMCID: PMC3170916, DOI: 10.1016/j.chom.2011.06.010.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBorrelia burgdorferiCell Migration AssaysCloning, MolecularComplement Membrane Attack ComplexComplement Pathway, Mannose-Binding LectinFemaleGene SilencingHemolysisHumansImmunization, PassiveImmunotherapy, ActiveInsect ProteinsIxodesLarvaLyme DiseaseMiceMice, Inbred C3HMolecular Sequence DataNeutrophilsNymphPhagocytosisRabbitsRecombinant ProteinsSalivaSalivary Proteins and PeptidesSequence AlignmentConceptsComplement cascadeLyme disease agent Borrelia burgdorferiImpaired neutrophil phagocytosisTick salivary proteinsVector-borne pathogensLyme disease agentMammalian infectionVector colonizationVertebrate hostsTick midgutAlternative complement pathwayBorrelia transmissionComplement-mediated killingVector proteinNeutrophil phagocytosisEssential rolePathway inhibitorComplement pathwayDisease agentsSalivary proteinsBorrelia burgdorferiLectin inhibitorsProteinCascadeIxodes ticks
2002
A novel family of anticoagulants from the saliva of Ixodes scapularis
Narasimhan S, Koski RA, Beaulieu B, Anderson JF, Ramamoorthi N, Kantor F, Cappello M, Fikrig E. A novel family of anticoagulants from the saliva of Ixodes scapularis. Insect Molecular Biology 2002, 11: 641-650. PMID: 12421422, DOI: 10.1046/j.1365-2583.2002.00375.x.Peer-Reviewed Original ResearchConceptsN-terminal amino acid sequenceSalivary gland cDNA libraryAmino acid sequenceN-terminal sequenceCDNA libraryAcid sequenceAnticoagulant proteinsIxodes scapularisMolecular approachesTerminal sequenceIntrinsic pathwayEscherichia coliSpecific inhibitorNovel familyProteinSalp14Different functionsTick salivaSequenceBiological activityDose-dependent mannerProtease inhibitorsReversed-phase HPLCParaloguesScapularis
1999
Ancylostoma secreted protein 2: cloning and characterization of a second member of a family of nematode secreted proteins from Ancylostoma caninum
Hawdon J, Narasimhan S, Hotez P. Ancylostoma secreted protein 2: cloning and characterization of a second member of a family of nematode secreted proteins from Ancylostoma caninum. Molecular And Biochemical Parasitology 1999, 99: 149-165. PMID: 10340481, DOI: 10.1016/s0166-6851(99)00011-0.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAncylostomaAncylostomatoideaAnimalsAntibodies, HelminthAntibody SpecificityAspartic Acid EndopeptidasesBase SequenceBlotting, SouthernBlotting, WesternCloning, MolecularDNA, HelminthDogsGenes, HelminthHelminth ProteinsLarvaMolecular Sequence DataRecombinant ProteinsSequence AlignmentConceptsHost-specific signalsLife history stagesFamilies of nematodesEarly eventsNematode physiologyHistory stagesHookworm Ancylostoma caninumParasitic speciesCDNA encodesCDNA libraryParasitic nematodesGene productsDevelopmental eventsInfective third-stage larvaeDiverse familyNematodesAmino acidsExcretory/secretory productsProtein 2ProteinSpecific signalsDatabase searchingAncylostoma caninumThird-stage larvaeImportant functions
1997
Prohibitin, a putative negative control element present in Pneumocystis carinii
Narasimhan S, Armstrong M, McClung J, Richards F, Spicer E. Prohibitin, a putative negative control element present in Pneumocystis carinii. Infection And Immunity 1997, 65: 5125-5130. PMID: 9393805, PMCID: PMC175738, DOI: 10.1128/iai.65.12.5125-5130.1997.Peer-Reviewed Original ResearchConceptsProhibitin geneCDNA clonesP. carinii genesAmino acid sequence homologyNegative control elementBlot analysisSouthern blot analysisCarinii genesCDNA libraryProhibitin proteinGene productsSequence homologyCell cycleMammalian sourcesWestern blot analysisGenesProhibitinG1 phaseCell proliferationHuman fibroblastsControl elementsProteinClonesPotential roleRegulation