The Mechanism of Action of Steroidogenic Acute Regulatory Protein (StAR) StAR ACTS ON THE OUTSIDE OF MITOCHONDRIA TO STIMULATE STEROIDOGENESIS*
Arakane F, Kallen C, Watari H, Foster J, Sepuri N, Pain D, Stayrook S, Lewis M, Gerton G, Strauss J. The Mechanism of Action of Steroidogenic Acute Regulatory Protein (StAR) StAR ACTS ON THE OUTSIDE OF MITOCHONDRIA TO STIMULATE STEROIDOGENESIS*. Journal Of Biological Chemistry 1998, 273: 16339-16345. PMID: 9632696, DOI: 10.1074/jbc.273.26.16339.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCholesterolCorpus LuteumCOS CellsFemaleMembrane ProteinsMitochondriaMutagenesis, Site-DirectedPhosphoproteinsPregnenoloneRecombinant ProteinsTransfectionConceptsCOS-1 cellsMitochondrial targeting sequenceWild-type StARSteroidogenic acute regulatory proteinStAR preproteinHis-tagMitochondrial membraneIsolated mitochondriaNH2-terminal mitochondrial targeting sequenceCOS-1Target sequenceRegulatory proteinsMechanism of StAR's actionIn vitro import assayStAR proteinStAR's actionCytoplasm of transfected COS-1 cellsProteins associated with mitochondriaCholesterol side-chain cleavage systemLipoid congenital adrenal hyperplasiaSide-chain cleavage systemLocalized to mitochondriaN-62 StARHis-tagged proteinsAbsence of cytosol