Steroidogenic Acute Regulatory Protein (StAR) Is A Sterol Transfer Protein*
Kallen C, Billheimer J, Summers S, Stayrook S, Lewis M, Strauss J. Steroidogenic Acute Regulatory Protein (StAR) Is A Sterol Transfer Protein*. Journal Of Biological Chemistry 1998, 273: 26285-26288. PMID: 9756854, DOI: 10.1074/jbc.273.41.26285.Peer-Reviewed Original ResearchConceptsSterol carrier protein-2Steroidogenic acute regulatory proteinStAR proteinRegulatory proteinsSterol transferCholesterol side-chain cleavage enzyme systemMitochondrial targeting sequenceSterol transfer activityTransfer proteinSterol transfer proteinsOuter mitochondrial membraneTrypsin-treated mitochondriaAmino acid residuesLipid transfer proteinsTransfer of cholesterolDonor membranesSubstrate cholesterolInner membraneMitochondrial membraneIsolated mitochondriaTarget sequenceN-terminalTemperature-dependent mannerAcid residuesPhosphatidylcholine transferThe Mechanism of Action of Steroidogenic Acute Regulatory Protein (StAR) StAR ACTS ON THE OUTSIDE OF MITOCHONDRIA TO STIMULATE STEROIDOGENESIS*
Arakane F, Kallen C, Watari H, Foster J, Sepuri N, Pain D, Stayrook S, Lewis M, Gerton G, Strauss J. The Mechanism of Action of Steroidogenic Acute Regulatory Protein (StAR) StAR ACTS ON THE OUTSIDE OF MITOCHONDRIA TO STIMULATE STEROIDOGENESIS*. Journal Of Biological Chemistry 1998, 273: 16339-16345. PMID: 9632696, DOI: 10.1074/jbc.273.26.16339.Peer-Reviewed Original ResearchConceptsCOS-1 cellsMitochondrial targeting sequenceWild-type StARSteroidogenic acute regulatory proteinStAR preproteinHis-tagMitochondrial membraneIsolated mitochondriaNH2-terminal mitochondrial targeting sequenceCOS-1Target sequenceRegulatory proteinsMechanism of StAR's actionIn vitro import assayStAR proteinStAR's actionCytoplasm of transfected COS-1 cellsProteins associated with mitochondriaCholesterol side-chain cleavage systemLipoid congenital adrenal hyperplasiaSide-chain cleavage systemLocalized to mitochondriaN-62 StARHis-tagged proteinsAbsence of cytosolSteroidogenic acute regulatory protein (StAR) acts on the outside of mitochondria to stimulate steroidogenesis
Arakane F, Kallen C, Watari H, Stayrook S, Lewis M, Strauss J. Steroidogenic acute regulatory protein (StAR) acts on the outside of mitochondria to stimulate steroidogenesis. Endocrine Research 1998, 24: 463-468. PMID: 9888526, DOI: 10.3109/07435809809032634.Peer-Reviewed Original ResearchConceptsCOS-1 cellsMitochondrial targeting sequenceWild-type StARSteroidogenic acute regulatory proteinHis-tagMitochondrial membraneIsolated mitochondriaN-terminal mitochondrial targeting sequenceTarget sequenceCOS-1Regulatory proteinsCytoplasm of transfected COS-1 cellsCholesterol side-chain cleavage systemLipoid congenital adrenal hyperplasiaSide-chain cleavage systemLocalized to mitochondriaInner mitochondrial membraneHis-tagged proteinsDelivery of cholesterolMitochondrial importPoint mutantsN-terminalStAR proteinCleavage systemE. coli