2014
New Structural Insights into High Affinity Membrane Binding By Coagulation Factor V/Va
Kumar S, Stayrook S, Huntington J, Camire R, Krishnaswamy S. New Structural Insights into High Affinity Membrane Binding By Coagulation Factor V/Va. Blood 2014, 124: 4216. DOI: 10.1182/blood.v124.21.4216.4216.Peer-Reviewed Original ResearchSmall-angle X-ray scattering envelopeMembrane bindingPS-containing membranesC-domainMembrane binding functionMembrane-bound configurationHigh-affinity membrane bindingX-ray structureC2 domainBinding to membranesFactor VaBinding variantsBinding to PS-containing membranesC1 domainV-formBinding functionSmall-angle X-ray scatteringConformational rearrangementsEnergetic expenditureMembrane affinityStructural insightsAffinity interactionSites of vascular damageConformational changesPublished structures
2005
X-ray Structure of a Water-soluble Analog of the Membrane Protein Phospholamban: Sequence Determinants Defining the Topology of Tetrameric and Pentameric Coiled Coils
Slovic A, Stayrook S, North B, DeGrado W. X-ray Structure of a Water-soluble Analog of the Membrane Protein Phospholamban: Sequence Determinants Defining the Topology of Tetrameric and Pentameric Coiled Coils. Journal Of Molecular Biology 2005, 348: 777-787. PMID: 15826670, DOI: 10.1016/j.jmb.2005.02.040.Peer-Reviewed Original ResearchConceptsHeptad sequence repeatSequence repeatWater-soluble variantCoiled-coilSequence determinationTransmembrane proteinsTruncated constructsPentameric transmembrane proteinsReticulum membraneStable tetramersTetrameric structureLeu residuesCrystal structurePentameric formX-ray structureInterfacial hydrogen bondsProteinHydrogen bondsResiduesRepeatsMembrane protein phospholambanGCN4Water-soluble analogX-rayHeptad
2003
X-ray Structure of a Maquette Scaffold
Huang S, Gibney B, Stayrook S, Dutton P, Lewis M. X-ray Structure of a Maquette Scaffold. Journal Of Molecular Biology 2003, 326: 1219-1225. PMID: 12589764, DOI: 10.1016/s0022-2836(02)01441-9.Peer-Reviewed Original ResearchConceptsLeft-handed coiled-coilsCoiled-coilAnti-parallel four-helix bundleHeptad repeat patternFour-alpha-helical bundleMaquette scaffoldFour-helix bundleHeme-binding propertiesX-ray structureMAD phasingHeme binding pocketIdentical helicesResidue positionsHeptad repeatHydrophobic residuesPacking interfaceProtein scaffoldEngineering criteriaHeptadMaquettesRepeat patternHelixDesign criteriaX-rayInitial design