2022
De novo protein design of photochemical reaction centers
Ennist N, Zhao Z, Stayrook S, Discher B, Dutton P, Moser C. De novo protein design of photochemical reaction centers. Nature Communications 2022, 13: 4937. PMID: 35999239, PMCID: PMC9399245, DOI: 10.1038/s41467-022-32710-5.Peer-Reviewed Original ResearchConceptsCharge separationSolar-to-fuel energy conversionReaction centerLight-driven charge separationX-ray crystal structurePhotosynthetic reaction center proteinCharge separation lifetimeSolar fuel productionTransient absorption spectroscopyPhotosynthetic reaction centersPhotochemical charge separationModify natural proteinsPhotochemical reaction centerReaction center proteinCluster oxidationRedox centersCrystal structureAbsorption spectroscopyElectron transfer activityNatural protein structuresDe novo protein designPhotosynthetic protein complexesEnergy conversionX-rayProtein framework
2008
Structural basis for the function and inhibition of an influenza virus proton channel
Stouffer A, Acharya R, Salom D, Levine A, Di Costanzo L, Soto C, Tereshko V, Nanda V, Stayrook S, DeGrado W. Structural basis for the function and inhibition of an influenza virus proton channel. Nature 2008, 451: 596-599. PMID: 18235504, PMCID: PMC3889492, DOI: 10.1038/nature06528.Peer-Reviewed Original Research
2005
X-ray Structure of a Water-soluble Analog of the Membrane Protein Phospholamban: Sequence Determinants Defining the Topology of Tetrameric and Pentameric Coiled Coils
Slovic A, Stayrook S, North B, DeGrado W. X-ray Structure of a Water-soluble Analog of the Membrane Protein Phospholamban: Sequence Determinants Defining the Topology of Tetrameric and Pentameric Coiled Coils. Journal Of Molecular Biology 2005, 348: 777-787. PMID: 15826670, DOI: 10.1016/j.jmb.2005.02.040.Peer-Reviewed Original ResearchConceptsHeptad sequence repeatSequence repeatWater-soluble variantCoiled-coilSequence determinationTransmembrane proteinsTruncated constructsPentameric transmembrane proteinsReticulum membraneStable tetramersTetrameric structureLeu residuesCrystal structurePentameric formX-ray structureInterfacial hydrogen bondsProteinHydrogen bondsResiduesRepeatsMembrane protein phospholambanGCN4Water-soluble analogX-rayHeptad
2003
X-ray Structure of a Maquette Scaffold
Huang S, Gibney B, Stayrook S, Dutton P, Lewis M. X-ray Structure of a Maquette Scaffold. Journal Of Molecular Biology 2003, 326: 1219-1225. PMID: 12589764, DOI: 10.1016/s0022-2836(02)01441-9.Peer-Reviewed Original ResearchConceptsLeft-handed coiled-coilsCoiled-coilAnti-parallel four-helix bundleHeptad repeat patternFour-alpha-helical bundleMaquette scaffoldFour-helix bundleHeme-binding propertiesX-ray structureMAD phasingHeme binding pocketIdentical helicesResidue positionsHeptad repeatHydrophobic residuesPacking interfaceProtein scaffoldEngineering criteriaHeptadMaquettesRepeat patternHelixDesign criteriaX-rayInitial design