X-Ray Structure of an Anticoagulant RNA Aptamer Bound to Factor Xa. Structural Basis for Its Ability to Disrupt Interactions Between Xa and Va within Prothrombinase
Kumar S, Sullenger B, Stayrook S, Krishnaswamy S. X-Ray Structure of an Anticoagulant RNA Aptamer Bound to Factor Xa. Structural Basis for Its Ability to Disrupt Interactions Between Xa and Va within Prothrombinase. Blood 2014, 124: 4232. DOI: 10.1182/blood.v124.21.4232.4232.Peer-Reviewed Original ResearchRNA aptamersC-terminusBinding to factor VIIIaIntrinsic XaseBase-paired stemActive site functionAssembly of prothrombinaseFactor Xa and factor VaSELEX screeningMolecular replacementInteracting proteinsProteinase domainFunctional characterizationAptamer bindingCatalytic Ser195Disrupt interactionsA-resolutionFactor XaHeparin bindingStructural basisAutolysis loopNucleotideInteracting speciesNucleotide basesSolvent accessible surface area