2007
Structural Analysis of Lac Repressor Bound to Allosteric Effectors
Daber R, Stayrook S, Rosenberg A, Lewis M. Structural Analysis of Lac Repressor Bound to Allosteric Effectors. Journal Of Molecular Biology 2007, 370: 609-619. PMID: 17543986, PMCID: PMC2715899, DOI: 10.1016/j.jmb.2007.04.028.Peer-Reviewed Original ResearchConceptsHydrogen bond networkHydrogen bondsExtensive hydrogen-bonding networkWater-mediated hydrogen bond networkSugar ringBond networkCrystal structureHydroxyl groupsAllosteric transitionEffector moleculesAnti-inducerSmall moleculesAtomic detailMoleculesStructural conformationC-terminal sub-domainBondsApo-repressorHydrogenHydroxylRepressor functionLac operonLac repressorN-terminalRepressor
1998
Steroidogenic Acute Regulatory Protein (StAR) Is A Sterol Transfer Protein*
Kallen C, Billheimer J, Summers S, Stayrook S, Lewis M, Strauss J. Steroidogenic Acute Regulatory Protein (StAR) Is A Sterol Transfer Protein*. Journal Of Biological Chemistry 1998, 273: 26285-26288. PMID: 9756854, DOI: 10.1074/jbc.273.41.26285.Peer-Reviewed Original ResearchConceptsSterol carrier protein-2Steroidogenic acute regulatory proteinStAR proteinRegulatory proteinsSterol transferCholesterol side-chain cleavage enzyme systemMitochondrial targeting sequenceSterol transfer activityTransfer proteinSterol transfer proteinsOuter mitochondrial membraneTrypsin-treated mitochondriaAmino acid residuesLipid transfer proteinsTransfer of cholesterolDonor membranesSubstrate cholesterolInner membraneMitochondrial membraneIsolated mitochondriaTarget sequenceN-terminalTemperature-dependent mannerAcid residuesPhosphatidylcholine transferSteroidogenic acute regulatory protein (StAR) acts on the outside of mitochondria to stimulate steroidogenesis
Arakane F, Kallen C, Watari H, Stayrook S, Lewis M, Strauss J. Steroidogenic acute regulatory protein (StAR) acts on the outside of mitochondria to stimulate steroidogenesis. Endocrine Research 1998, 24: 463-468. PMID: 9888526, DOI: 10.3109/07435809809032634.Peer-Reviewed Original ResearchConceptsCOS-1 cellsMitochondrial targeting sequenceWild-type StARSteroidogenic acute regulatory proteinHis-tagMitochondrial membraneIsolated mitochondriaN-terminal mitochondrial targeting sequenceTarget sequenceCOS-1Regulatory proteinsCytoplasm of transfected COS-1 cellsCholesterol side-chain cleavage systemLipoid congenital adrenal hyperplasiaSide-chain cleavage systemLocalized to mitochondriaInner mitochondrial membraneHis-tagged proteinsDelivery of cholesterolMitochondrial importPoint mutantsN-terminalStAR proteinCleavage systemE. coli