2014
New Structural Insights into High Affinity Membrane Binding By Coagulation Factor V/Va
Kumar S, Stayrook S, Huntington J, Camire R, Krishnaswamy S. New Structural Insights into High Affinity Membrane Binding By Coagulation Factor V/Va. Blood 2014, 124: 4216. DOI: 10.1182/blood.v124.21.4216.4216.Peer-Reviewed Original ResearchSmall-angle X-ray scattering envelopeMembrane bindingPS-containing membranesC-domainMembrane binding functionMembrane-bound configurationHigh-affinity membrane bindingX-ray structureC2 domainBinding to membranesFactor VaBinding variantsBinding to PS-containing membranesC1 domainV-formBinding functionSmall-angle X-ray scatteringConformational rearrangementsEnergetic expenditureMembrane affinityStructural insightsAffinity interactionSites of vascular damageConformational changesPublished structures
2011
High Resolution X-Ray Structure of Snake Venom Factor V: Evolution of a Hemostatic Cofactor to a Toxin Poised to Inflict Maximal Damage to Mammalian Blood Coagulation
Kumar S, Stayrook S, Huntington J, Camire R, Krishnaswamy S. High Resolution X-Ray Structure of Snake Venom Factor V: Evolution of a Hemostatic Cofactor to a Toxin Poised to Inflict Maximal Damage to Mammalian Blood Coagulation. Blood 2011, 118: 375. DOI: 10.1182/blood.v118.21.375.375.Peer-Reviewed Original ResearchC2 domainA2 domainVenom proteinsB domainVenom of Pseudonaja textilisMembrane-dependent reactionsA1-A2-B-A3-C1-C2Absence of membranesMammalian coagulationSequence alignmentMammalian blood coagulationDomain organizationMolecular replacementRegulating blood coagulationToxin repertoireProtruding loopBinding to membranesHydrophobic residuesProteolytic processingBind membranesResolution structureMembrane bindingA-resolutionActivation of human prothrombinProteolytic activity