2024
Beyond Earth’s bounds: navigating the frontiers of Assisted Reproductive Technologies (ART) in space
Chaplia O, Mathyk B, Nichols-Burns S, Basar M, Halicigil C. Beyond Earth’s bounds: navigating the frontiers of Assisted Reproductive Technologies (ART) in space. Reproductive Biology And Endocrinology 2024, 22: 123. PMID: 39394617, PMCID: PMC11468284, DOI: 10.1186/s12958-024-01290-y.Peer-Reviewed Original ResearchConceptsAssisted reproductive technologyIn vitro embryo developmentReproductive technologyRadiation-induced damageGamete handlingClinical embryologistsReproductive endocrinologistsTemperature limitEmbryo cultureReproductive medicineLow Earth orbitEmbryo developmentDeep space travelEarth orbitSpace environmentInterplanetary speciesEarth-boundMicrofluidic devicesCryopreservation
2019
A step towards the automation of intracytoplasmic sperm injection: real time confirmation of mouse and human oocyte penetration and viability by electrical resistance measurement
Mor A, Zhang M, Esencan E, Simsek B, Nichols-Burns SM, Liu Y, Lo J, Kelk DA, Flores V, Gao XB, Seli E. A step towards the automation of intracytoplasmic sperm injection: real time confirmation of mouse and human oocyte penetration and viability by electrical resistance measurement. Fertility And Sterility 2019, 113: 234-236. PMID: 31883732, DOI: 10.1016/j.fertnstert.2019.09.023.Peer-Reviewed Original ResearchConceptsIntracytoplasmic sperm injectionICSI pipetteSperm injectionOocyte penetrationIntact oolemmaOocyte retrieval procedureMann-Whitney testMouse oocytesOocyte retrievalFemale miceMAIN OUTCOMEAcademic centersImmature oocytesHuman oocytesMature oocytesObjective toolMiceOocytesWomenVisual assessmentPipetteInjectionOolemmaStep 1GroupRepeated semen exposure decreases cervicovaginal SIVmac251 infection in rhesus macaques
Abdulhaqq S, Martinez M, Kang G, Rodriguez I, Nichols S, Beaumont D, Joseph J, Azzoni L, Yin X, Wise M, Weiner D, Liu Q, Foulkes A, Münch J, Kirchhoff F, Coutifaris C, Tomaras G, Sariol C, Marx P, Li Q, Kraiselburd E, Montaner L. Repeated semen exposure decreases cervicovaginal SIVmac251 infection in rhesus macaques. Nature Communications 2019, 10: 3753. PMID: 31434895, PMCID: PMC6704120, DOI: 10.1038/s41467-019-11814-5.Peer-Reviewed Original ResearchConceptsCD4+ T cellsFemale reproductive tractSemen exposureT cellsIncreased infiltration of CD4+ T cellsMale-to-female HIV transmissionCirculating CD4+ T cellsInfiltration of CD4+ T cellsExposure to semenEffect of semenNon-human primatesExpression of Mx1Anti-HIV mechanismsCervicovaginal mucosaSIVmac251 challengeSIVmac251 infectionCCR5 expressionIn vivo evidenceHIV acquisitionHIV infectionHIV transmissionAssociated with activationIncreased infiltrationRhesus macaquesReproductive tract
2015
Comparison of Whole Ovary Cryotreatments for Fertility Preservation
Lotz L, Hauenstein T, Nichols-Burns S, Strissel P, Hoffmann I, Findeklee S, Dittrich R, Beckmann M, Oppelt P. Comparison of Whole Ovary Cryotreatments for Fertility Preservation. Reproduction In Domestic Animals 2015, 50: 958-964. PMID: 26446780, DOI: 10.1111/rda.12615.Peer-Reviewed Original ResearchConceptsWhole ovary cryopreservationWhole ovariesOvary cryopreservationSlow freezeFresh controlFertility preservationFollicle numberApoptotic folliclesHistological evaluationKrebs-RingerRadiotherapy treatmentPig ovariesOvariesSlow-freezing methodsSignificant differencesFunctional integrityFolliclesFurther investigationFresh tissueSpecimen jarOverall decrease
2014
Serial Cervicovaginal Exposures With Replication-Deficient SIVsm Induce Higher Dendritic Cell (pDC) and CD4+ T-Cell Infiltrates Not Associated With Prevention but a More Severe SIVmac251 Infection of Rhesus Macaques
Abdulhaqq S, Martinez M, Kang G, Foulkes A, Rodriguez I, Nichols S, Hunter M, Sariol C, Ruiz L, Ross B, Yin X, Speicher D, Haase A, Marx P, Li Q, Kraiselburd E, Montaner L. Serial Cervicovaginal Exposures With Replication-Deficient SIVsm Induce Higher Dendritic Cell (pDC) and CD4+ T-Cell Infiltrates Not Associated With Prevention but a More Severe SIVmac251 Infection of Rhesus Macaques. JAIDS Journal Of Acquired Immune Deficiency Syndromes 2014, 65: 405-413. PMID: 24226059, PMCID: PMC3943721, DOI: 10.1097/qai.0000000000000047.Peer-Reviewed Original ResearchConceptsPlasmacytoid dendritic cellsSimian immunodeficiency virusT cell infiltrationDendritic cellsCD4 T cell lossCD4+ T cell infiltrationPlasmacytoid dendritic cell recruitmentRate of CD4 declineInfection of rhesus macaquesCD4 T cell infiltrationRate of viral replicationT cell lossPlasma viral loadRhesus macaquesCD4 T cellsCD4 T lymphocytesImmune cell infiltrationIndian rhesus macaquesAssociated with preventionCD4 countVaginal exposureCD4 declineImmunodeficiency virusVaginal tissueT cells
2009
Long-distance transportation of primate embryos developing in culture: a preliminary study
Nichols S, Harvey A, Gierbolini L, Gonzalez-Martinez J, Brenner C, Bavister B. Long-distance transportation of primate embryos developing in culture: a preliminary study. Reproductive BioMedicine Online 2009, 20: 365-370. PMID: 20093089, PMCID: PMC2829350, DOI: 10.1016/j.rbmo.2009.11.026.Peer-Reviewed Original ResearchConceptsPrimate embryosNon-human primate embryosPrimate Research CentreCleavage-stage embryosRhesus monkey embryosPreimplantation stagesMonkey embryosHuman infertilityCulture mediumAdvanced stageProcess of productionDevelopmental abilityTransport incubatorStem cellsDistant sitesProlonged cultureAdvanced stage of developmentPreliminary studyFresh culture mediumOocytesEmbryosIVFWayne State UniversityInfertilityOvernight courierInner Cell Mass Localization of NANOG Precedes OCT3/4 in Rhesus Monkey Blastocysts
Harvey A, Armant D, Bavister B, Nichols S, Brenner C. Inner Cell Mass Localization of NANOG Precedes OCT3/4 in Rhesus Monkey Blastocysts. Stem Cells And Development 2009, 18: 1451-1458. PMID: 19537945, PMCID: PMC3016202, DOI: 10.1089/scd.2009.0122.Peer-Reviewed Original ResearchConceptsInner cell massInner cell masses of blastocystsRhesus monkey blastocystsOCT4 proteinExpression of CDX2Compacted morulaePig embryosBlastocystRegulatory markersOct4 expressionMouse embryosTrophectodermNanogNanog proteinCell massInvestigate local patternsPrimate developmentMiceRhesusMarkersExpressionLineage-specificEmbryosRegulatory mechanismsComplex interaction networksEffects of in vitro maturation and age on oocyte quality in the rhesus macaque Macaca mulatta
Nichols S, Gierbolini L, Gonzalez-Martinez J, Bavister B. Effects of in vitro maturation and age on oocyte quality in the rhesus macaque Macaca mulatta. Fertility And Sterility 2009, 93: 1591-1600. PMID: 19249021, DOI: 10.1016/j.fertnstert.2008.12.141.Peer-Reviewed Original ResearchConceptsIn vitro matured oocytesIn vitro maturationMetaphase II oocytesIVO oocytesRegimen BRegimen AII oocytesOocyte qualityImmature oocytesEffects of in vitro maturationEvaluate oocyte qualityAbnormal chromosome alignmentYoung femalesOld rhesus macaquesConfocal microscopyMacaque oocytesPrimate oocytesDevelopmental competenceStimulated cyclesOocyte spindleRegimenRegimensPrimate modelOocytesRhesus macaques
2006
Comparison of protocols for cryopreservation of rhesus monkey spermatozoa by post-thaw motility recovery and hyperactivation
Nichols S, Bavister B. Comparison of protocols for cryopreservation of rhesus monkey spermatozoa by post-thaw motility recovery and hyperactivation. Reproduction Fertility And Development 2006, 18: 777-780. PMID: 17032586, DOI: 10.1071/rd06019.Peer-Reviewed Original ResearchConceptsIn vitro fertilisationGene bankPost-thaw motility rateSperm longevityCryopreservation of spermatozoaSperm motilityRhesus spermatozoaMonkey spermatozoaMotility rateResponses to chemical activationSpermatozoaDegrees CMotility recoveryMotilitySpermGenesRhesus macaquesExposure to activitiesCryopreservation protocolsCryopreservation techniquesCryopreservationActivityEjaculationStatistical analysisHyperactivation
2005
Ovarian senescence in the rhesus monkey (Macaca mulatta)
Nichols S, Bavister B, Brenner C, Didier P, Harrison R, Kubisch H. Ovarian senescence in the rhesus monkey (Macaca mulatta). Human Reproduction 2005, 20: 79-83. PMID: 15498779, PMCID: PMC4968694, DOI: 10.1093/humrep/deh576.Peer-Reviewed Original ResearchConceptsOvarian morphologyOvarian senescenceOld World primatesNumbers of primordial folliclesTime of menstrual cycleRhesus monkeysAge groupsWorld primatesPregnancy ratePrimordial folliclesMenstrual cycleFemales aged 1Primary folliclesStromal tissueAtretic folliclesReproductive ageReproductive senescenceMenopausal transitionAged 1FolliclesRhesusHuman femalesMacaca mulattaAgeOvary
2004
Non-human primates as a model for reproductive aging and human infertility.
Brenner C, Nichols S, Jacoby E, Bavister B. Non-human primates as a model for reproductive aging and human infertility. Gynecologic And Obstetric Investigation 2004, 57: 21-3. PMID: 14971419.Peer-Reviewed Original Research
2003
Calcitonin‐like immunoreactivity in serum and tissues of the bonnethead shark, Sphyrna tiburo
Nichols S, Gelsleichter J, Manire C, Cailliet G. Calcitonin‐like immunoreactivity in serum and tissues of the bonnethead shark, Sphyrna tiburo. Journal Of Experimental Zoology Part A Ecological And Integrative Physiology 2003, 298A: 150-161. PMID: 12884277, DOI: 10.1002/jez.a.10271.Peer-Reviewed Original ResearchConceptsS. tiburoSphyrna tiburoBonnethead sharksHeterologous enzyme-linked immunosorbent assaySerum calcitonin concentrationEnzyme-linked immunosorbent assayMature femalesSphyrnaBonnetheadSharksYolk dependenceElasmobranchsNonmammalian vertebratesReproductionEmbryonic developmentAnimal reproductionEarly stages of gestationRegulate certain aspectsPeptide hormonesStages of gestationYolk digestionCalcium homeostasisCalcitonin-like immunoreactivityMaternal sources