2019
Repeated semen exposure decreases cervicovaginal SIVmac251 infection in rhesus macaques
Abdulhaqq S, Martinez M, Kang G, Rodriguez I, Nichols S, Beaumont D, Joseph J, Azzoni L, Yin X, Wise M, Weiner D, Liu Q, Foulkes A, Münch J, Kirchhoff F, Coutifaris C, Tomaras G, Sariol C, Marx P, Li Q, Kraiselburd E, Montaner L. Repeated semen exposure decreases cervicovaginal SIVmac251 infection in rhesus macaques. Nature Communications 2019, 10: 3753. PMID: 31434895, PMCID: PMC6704120, DOI: 10.1038/s41467-019-11814-5.Peer-Reviewed Original ResearchConceptsCD4+ T cellsFemale reproductive tractSemen exposureT cellsIncreased infiltration of CD4+ T cellsMale-to-female HIV transmissionCirculating CD4+ T cellsInfiltration of CD4+ T cellsExposure to semenEffect of semenNon-human primatesExpression of Mx1Anti-HIV mechanismsCervicovaginal mucosaSIVmac251 challengeSIVmac251 infectionCCR5 expressionIn vivo evidenceHIV acquisitionHIV infectionHIV transmissionAssociated with activationIncreased infiltrationRhesus macaquesReproductive tract
2014
Serial Cervicovaginal Exposures With Replication-Deficient SIVsm Induce Higher Dendritic Cell (pDC) and CD4+ T-Cell Infiltrates Not Associated With Prevention but a More Severe SIVmac251 Infection of Rhesus Macaques
Abdulhaqq S, Martinez M, Kang G, Foulkes A, Rodriguez I, Nichols S, Hunter M, Sariol C, Ruiz L, Ross B, Yin X, Speicher D, Haase A, Marx P, Li Q, Kraiselburd E, Montaner L. Serial Cervicovaginal Exposures With Replication-Deficient SIVsm Induce Higher Dendritic Cell (pDC) and CD4+ T-Cell Infiltrates Not Associated With Prevention but a More Severe SIVmac251 Infection of Rhesus Macaques. JAIDS Journal Of Acquired Immune Deficiency Syndromes 2014, 65: 405-413. PMID: 24226059, PMCID: PMC3943721, DOI: 10.1097/qai.0000000000000047.Peer-Reviewed Original ResearchConceptsPlasmacytoid dendritic cellsSimian immunodeficiency virusT cell infiltrationDendritic cellsCD4 T cell lossCD4+ T cell infiltrationPlasmacytoid dendritic cell recruitmentRate of CD4 declineInfection of rhesus macaquesCD4 T cell infiltrationRate of viral replicationT cell lossPlasma viral loadRhesus macaquesCD4 T cellsCD4 T lymphocytesImmune cell infiltrationIndian rhesus macaquesAssociated with preventionCD4 countVaginal exposureCD4 declineImmunodeficiency virusVaginal tissueT cells
2010
Recovery of Multiple Ovulation Products via Uterine Flushing of Superovulated Rhesus Macaques.
Nichols S, Gierbolini L, Gonzalez-Martinez J, Bavister B. Recovery of Multiple Ovulation Products via Uterine Flushing of Superovulated Rhesus Macaques. Biology Of Reproduction 2010, 83: 680-680. DOI: 10.1093/biolreprod/83.s1.680.Peer-Reviewed Original ResearchRhesus monkeysUnfertilized oocytesUterine flushingsRhesus macaquesEmbryonic stem cell linesStem cell linesSite of ovulationEarly embryo developmentRates of embryo recoveryCervical cannulationResponse to exogenous hormonesRodent modelsStandard in vivoHormonal profileMultiplication in vivoAssisted reproductionEmbryo characteristicsCell linesNon-human primatesRhesusEmbryo developmentEmbryo recoveryHuman reproductive issuesBlastocystUterine
2009
Effects of in vitro maturation and age on oocyte quality in the rhesus macaque Macaca mulatta
Nichols S, Gierbolini L, Gonzalez-Martinez J, Bavister B. Effects of in vitro maturation and age on oocyte quality in the rhesus macaque Macaca mulatta. Fertility And Sterility 2009, 93: 1591-1600. PMID: 19249021, DOI: 10.1016/j.fertnstert.2008.12.141.Peer-Reviewed Original ResearchConceptsIn vitro matured oocytesIn vitro maturationMetaphase II oocytesIVO oocytesRegimen BRegimen AII oocytesOocyte qualityImmature oocytesEffects of in vitro maturationEvaluate oocyte qualityAbnormal chromosome alignmentYoung femalesOld rhesus macaquesConfocal microscopyMacaque oocytesPrimate oocytesDevelopmental competenceStimulated cyclesOocyte spindleRegimenRegimensPrimate modelOocytesRhesus macaques
2006
Comparison of protocols for cryopreservation of rhesus monkey spermatozoa by post-thaw motility recovery and hyperactivation
Nichols S, Bavister B. Comparison of protocols for cryopreservation of rhesus monkey spermatozoa by post-thaw motility recovery and hyperactivation. Reproduction Fertility And Development 2006, 18: 777-780. PMID: 17032586, DOI: 10.1071/rd06019.Peer-Reviewed Original ResearchConceptsIn vitro fertilisationGene bankPost-thaw motility rateSperm longevityCryopreservation of spermatozoaSperm motilityRhesus spermatozoaMonkey spermatozoaMotility rateResponses to chemical activationSpermatozoaDegrees CMotility recoveryMotilitySpermGenesRhesus macaquesExposure to activitiesCryopreservation protocolsCryopreservation techniquesCryopreservationActivityEjaculationStatistical analysisHyperactivation