2019
A step towards the automation of intracytoplasmic sperm injection: real time confirmation of mouse and human oocyte penetration and viability by electrical resistance measurement
Mor A, Zhang M, Esencan E, Simsek B, Nichols-Burns SM, Liu Y, Lo J, Kelk DA, Flores V, Gao XB, Seli E. A step towards the automation of intracytoplasmic sperm injection: real time confirmation of mouse and human oocyte penetration and viability by electrical resistance measurement. Fertility And Sterility 2019, 113: 234-236. PMID: 31883732, DOI: 10.1016/j.fertnstert.2019.09.023.Peer-Reviewed Original ResearchConceptsIntracytoplasmic sperm injectionICSI pipetteSperm injectionOocyte penetrationIntact oolemmaOocyte retrieval procedureMann-Whitney testMouse oocytesOocyte retrievalFemale miceMAIN OUTCOMEAcademic centersImmature oocytesHuman oocytesMature oocytesObjective toolMiceOocytesWomenVisual assessmentPipetteInjectionOolemmaStep 1Group
2009
Long-distance transportation of primate embryos developing in culture: a preliminary study
Nichols S, Harvey A, Gierbolini L, Gonzalez-Martinez J, Brenner C, Bavister B. Long-distance transportation of primate embryos developing in culture: a preliminary study. Reproductive BioMedicine Online 2009, 20: 365-370. PMID: 20093089, PMCID: PMC2829350, DOI: 10.1016/j.rbmo.2009.11.026.Peer-Reviewed Original ResearchConceptsPrimate embryosNon-human primate embryosPrimate Research CentreCleavage-stage embryosRhesus monkey embryosPreimplantation stagesMonkey embryosHuman infertilityCulture mediumAdvanced stageProcess of productionDevelopmental abilityTransport incubatorStem cellsDistant sitesProlonged cultureAdvanced stage of developmentPreliminary studyFresh culture mediumOocytesEmbryosIVFWayne State UniversityInfertilityOvernight courierEffects of in vitro maturation and age on oocyte quality in the rhesus macaque Macaca mulatta
Nichols S, Gierbolini L, Gonzalez-Martinez J, Bavister B. Effects of in vitro maturation and age on oocyte quality in the rhesus macaque Macaca mulatta. Fertility And Sterility 2009, 93: 1591-1600. PMID: 19249021, DOI: 10.1016/j.fertnstert.2008.12.141.Peer-Reviewed Original ResearchConceptsIn vitro matured oocytesIn vitro maturationMetaphase II oocytesIVO oocytesRegimen BRegimen AII oocytesOocyte qualityImmature oocytesEffects of in vitro maturationEvaluate oocyte qualityAbnormal chromosome alignmentYoung femalesOld rhesus macaquesConfocal microscopyMacaque oocytesPrimate oocytesDevelopmental competenceStimulated cyclesOocyte spindleRegimenRegimensPrimate modelOocytesRhesus macaques