Early Infant Human Immunodeficiency Virus Type 1 Detection Suitable for Resource-Limited Settings with Multiple Circulating Subtypes by Use of Nested Three-Monoplex DNA PCR and Dried Blood Spots
Zhang Q, Wang L, Jiang Y, Fang L, Pan P, Gong S, Yao J, Tang YW, Vermund SH, Jia Y. Early Infant Human Immunodeficiency Virus Type 1 Detection Suitable for Resource-Limited Settings with Multiple Circulating Subtypes by Use of Nested Three-Monoplex DNA PCR and Dried Blood Spots. Journal Of Clinical Microbiology 2007, 46: 721-726. PMID: 18077639, PMCID: PMC2238144, DOI: 10.1128/jcm.01539-07.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsBloodBlotting, WesternChinaDNA PrimersDNA, ViralEnv Gene Products, Human Immunodeficiency VirusGag Gene Products, Human Immunodeficiency VirusHIV AntibodiesHIV InfectionsHIV-1HumansInfantInfant, NewbornPol Gene Products, Human Immunodeficiency VirusPolymerase Chain ReactionReproducibility of ResultsSensitivity and SpecificitySpecimen HandlingConceptsHIV-1 subtypesMonths of ageDiverse HIV-1 subtypesEarly infant HIV-1 diagnosisHuman immunodeficiency virus type 1 (HIV-1) infectionHIV-1 DNA PCRVirus type 1 infectionDNA PCRType 1 infectionHIV-1 diagnosisDiverse HIV-1Resource limited settingsAntibody enzyme immunoassayBlood spot samplesInfected mothersMaternal antibodiesCRF01-AEDBS specimensEpidemiologic dataHIV-1Deceased infantsInfantsCRF07-BCMultiple subtypesBlood spots