2015
Endosomal regulation of contact inhibition through the AMOT:YAP pathway
Cox CM, Mandell EK, Stewart L, Lu R, Johnson DL, McCarter SD, Tavares A, Runyan R, Ghosh S, Wilson JM. Endosomal regulation of contact inhibition through the AMOT:YAP pathway. Molecular Biology Of The Cell 2015, 26: 2673-2684. PMID: 25995376, PMCID: PMC4501364, DOI: 10.1091/mbc.e15-04-0224.Peer-Reviewed Original ResearchConceptsEndosomal membranesYAP activitySubconfluent cellsOrgan growth controlTranscription coactivator YesPhosphorylation-dependent regulationIntegral membrane proteinsConfluent cellsAmot proteinsEndosomal regulationContact-mediated inhibitionCytoplasmic domainEndotubinMembrane proteinsOvergrowth phenotypeInduces translocationAngiomotinYAP interactionDirect bindingGrowth controlContact inhibitionFamily membersCell growthYAPYAP pathwayPKCι interacts with Rab14 and modulates epithelial barrier function through regulation of claudin-2 levels
Lu R, Dalgalan D, Mandell EK, Parker SS, Ghosh S, Wilson JM. PKCι interacts with Rab14 and modulates epithelial barrier function through regulation of claudin-2 levels. Molecular Biology Of The Cell 2015, 26: 1523-1531. PMID: 25694446, PMCID: PMC4395131, DOI: 10.1091/mbc.e14-12-1613.Peer-Reviewed Original ResearchConceptsClaudin-2 levelsSmall GTPase Rab14Claudin-2 protein levelsClaudin-2Junction proteinsTransepithelial resistanceEpithelial polarityIntracellular punctaTight junction componentsTight junction proteinsPlasma membraneNormal assemblyRab14 expressionRab14Junction componentsPKCιKnockdownEpithelial barrier functionProtein levelsTight junctionsZO-1ProteinParacellular permeabilityClaudin-1APKC
2002
CK2 constitutively associates with and phosphorylates chicken erythroid ankyrin and regulates its ability to bind to spectrin
Ghosh S, Dorsey FC, Cox JV. CK2 constitutively associates with and phosphorylates chicken erythroid ankyrin and regulates its ability to bind to spectrin. Journal Of Cell Science 2002, 115: 4107-4115. PMID: 12356915, DOI: 10.1242/jcs.00102.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnkyrinsAntibody SpecificityCasein Kinase IICell MembraneChick EmbryoCytoskeletonDogsEnzyme InhibitorsEpithelial CellsErythroid Precursor CellsMacromolecular SubstancesPhosphoric Monoester HydrolasesPhosphorylationPhosphotransferasesPrecipitin TestsProtein BindingProtein IsoformsProtein Serine-Threonine KinasesSpectrinSubcellular FractionsConceptsErythroid ankyrinKidney epithelial cellsMadin-Darby canine kidney epithelial cellsCK2-specific inhibitorCanine kidney epithelial cellsEpithelial cellsPhosphorylation eventsPhosphatase inhibitorSpectrin cytoskeletonMembrane cytoskeletonPhosphorylation stateCK2Erythroid cellsAnkyrinCytoskeletonInhibitor studiesCell typesPhosphorylationSpectrinKinaseCellsFirst demonstrationVivoInhibitorsComplexes