Featured Publications
Cell death in development, maintenance, and diseases of the nervous system
Mercau ME, Patwa S, Bhat KPL, Ghosh S, Rothlin CV. Cell death in development, maintenance, and diseases of the nervous system. Seminars In Immunopathology 2022, 44: 725-738. PMID: 35508671, DOI: 10.1007/s00281-022-00938-4.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsCell deathTissue-level responsesNervous system homeostasisNervous systemCentral nervous system tumorsMolecular modalitiesAcute brain injuryNervous system tumorsChronic neurodegenerative diseasesSystem homeostasisDead cellsNew therapeutic strategiesNeurodegenerative diseasesMechanisms of disposalGlial cellsNovel understandingAdult neurogenesisSystem tumorsBrain injuryPathological responseDisease statesTherapeutic strategiesCellsRecent studiesDeath
2020
Astrocytes and microglia play orchestrated roles and respect phagocytic territories during neuronal corpse removal in vivo
Damisah EC, Hill RA, Rai A, Chen F, Rothlin CV, Ghosh S, Grutzendler J. Astrocytes and microglia play orchestrated roles and respect phagocytic territories during neuronal corpse removal in vivo. Science Advances 2020, 6: eaba3239. PMID: 32637606, PMCID: PMC7319765, DOI: 10.1126/sciadv.aba3239.Peer-Reviewed Original ResearchConceptsCorpse removalPrecise spatiotemporal resolutionApoptotic cell removalReceptor tyrosine kinasesGlial cellsOrchestrated rolesTyrosine kinaseApoptotic bodiesCell deathSpecialized roleCoordinated interactionPhagocytic interactionSingle cellsBrain homeostasisCellsCell removalIntravital optical imagingSpatiotemporal resolutionRole of phagocytesSynchronized fashionKinaseMarked delayRoleHomeostasisAstrocytes
2019
Cracking the Cell Death Code
Rothlin CV, Ghosh S. Cracking the Cell Death Code. Cold Spring Harbor Perspectives In Biology 2019, 12: a036343. PMID: 31548182, PMCID: PMC7197433, DOI: 10.1101/cshperspect.a036343.Peer-Reviewed Original Research
2018
Cenabis Bene: Treg Cells Invite Macrophages to Dine
Hughes LD, Ghosh S, Rothlin CV. Cenabis Bene: Treg Cells Invite Macrophages to Dine. Immunity 2018, 49: 579-582. PMID: 30332622, DOI: 10.1016/j.immuni.2018.10.002.Commentaries, Editorials and LettersPAR3–PAR6–atypical PKC polarity complex proteins in neuronal polarization
Hapak SM, Rothlin CV, Ghosh S. PAR3–PAR6–atypical PKC polarity complex proteins in neuronal polarization. Cellular And Molecular Life Sciences 2018, 75: 2735-2761. PMID: 29696344, PMCID: PMC11105418, DOI: 10.1007/s00018-018-2828-6.BooksConceptsPar3-Par6Neuronal polarityNeuronal polarizationPolarity complex proteinsAPKC kinase activityEukaryotic cell typesProtein complexesComplex proteinsKinase activityPar6APKCSignaling mechanismCell typesPar3Effector moleculesProteinImportant roleEffector functionsPolarityComplexesFundamental featuresPathwayRoleAssemblyCells
2017
Chronicles of Cell Death Foretold: Specificities in the Mechanism of Disposal
Hughes LD, Bosurgi L, Ghosh S, Rothlin CV. Chronicles of Cell Death Foretold: Specificities in the Mechanism of Disposal. Frontiers In Immunology 2017, 8: 1743. PMID: 29312294, PMCID: PMC5732325, DOI: 10.3389/fimmu.2017.01743.BooksApoptotic cellsApoptotic cell clearanceBillions of cellsExposure of phosphatidylserinePtdSer exposurePlasma membraneConstant remodelingOuter leafletSilent removalApoptotic bodiesUbiquitous signalCell clearanceMassive turnoverShedding of cellsTissue repairGrowth factorMechanisms of disposalApoptosisCellsEmergent propertiesPhagocytesHomeostasisBarrier surfacesPhosphatidylserineImmunological settingsDeath begets a new beginning
Bosurgi L, Hughes LD, Rothlin CV, Ghosh S. Death begets a new beginning. Immunological Reviews 2017, 280: 8-25. PMID: 29027219, PMCID: PMC5658037, DOI: 10.1111/imr.12585.BooksConceptsCell deathBillions of cellsApoptotic cell deathEnvironmental cuesSpecific functionsHomeostatic conditionsTissue microenvironmentApoptotic cellsDead cellsTissue repairGrowth factorCellsPhagocytosisPerpetual featureSevere tissue injuryDeathInductionAppropriate responseMicroenvironmentPhagocytesCuesMacrophage function in tissue repair and remodeling requires IL-4 or IL-13 with apoptotic cells
Bosurgi L, Cao YG, Cabeza-Cabrerizo M, Tucci A, Hughes LD, Kong Y, Weinstein JS, Licona-Limon P, Schmid ET, Pelorosso F, Gagliani N, Craft JE, Flavell RA, Ghosh S, Rothlin CV. Macrophage function in tissue repair and remodeling requires IL-4 or IL-13 with apoptotic cells. Science 2017, 356: 1072-1076. PMID: 28495875, PMCID: PMC5556699, DOI: 10.1126/science.aai8132.Peer-Reviewed Original ResearchConceptsApoptotic cellsTissue repair programChemotaxis genesTissue-resident macrophagesIL-4IL-13Tissue repairPattern recognition receptorsTissue repair genesCell adhesionRepair genesGenetic ablationCytokine-dependent inductionHelminth infectionsRecognition receptorsInduction of colitisGenesBroad repertoireSoluble cytokinesMacrophage functionCellsInductionHost responseEctopic activityInterleukin-4
2016
The TAM family receptor tyrosine kinase TYRO3 is a negative regulator of type 2 immunity
Chan PY, Carrera Silva EA, De Kouchkovsky D, Joannas LD, Hao L, Hu D, Huntsman S, Eng C, Licona-Limón P, Weinstein JS, Herbert DR, Craft JE, Flavell RA, Repetto S, Correale J, Burchard EG, Torgerson DG, Ghosh S, Rothlin CV. The TAM family receptor tyrosine kinase TYRO3 is a negative regulator of type 2 immunity. Science 2016, 352: 99-103. PMID: 27034374, PMCID: PMC4935984, DOI: 10.1126/science.aaf1358.Peer-Reviewed Original ResearchMeSH KeywordsAdaptive ImmunityAnimalsAsthmaBlood ProteinsDendritic CellsDisease Models, AnimalGene Knockout TechniquesHost-Parasite InteractionsHumansImmunity, InnateInterleukin-4MiceMice, Inbred C57BLMice, KnockoutNippostrongylusProtein SPyroglyphidaeReceptor Protein-Tyrosine KinasesStrongylida InfectionsT-LymphocytesConceptsType 2 immunityType 2 responsesType 2 cytokinesHuman dendritic cellsInnate immune cellsDendritic cellsAllergic diseasesImmune cellsT cellsAdaptive immunityInterleukin-4Host responseFunctional neutralizationGenetic ablationReceptor tyrosine kinasesImmunityProtective functionTyro3Tyrosine kinaseNegative regulatorPROS1CellsResponseCytokinesDisease
2013
T Cell-Derived Protein S Engages TAM Receptor Signaling in Dendritic Cells to Control the Magnitude of the Immune Response
Silva E, Chan PY, Joannas L, Errasti AE, Gagliani N, Bosurgi L, Jabbour M, Perry A, Smith-Chakmakova F, Mucida D, Cheroutre H, Burstyn-Cohen T, Leighton JA, Lemke G, Ghosh S, Rothlin CV. T Cell-Derived Protein S Engages TAM Receptor Signaling in Dendritic Cells to Control the Magnitude of the Immune Response. Immunity 2013, 39: 160-170. PMID: 23850380, PMCID: PMC4017237, DOI: 10.1016/j.immuni.2013.06.010.Peer-Reviewed Original ResearchMeSH KeywordsAdaptive ImmunityAnimalsCells, CulturedColitisCytokinesDendritic CellsFlow CytometryGene ExpressionHumansImmunoblottingLymphocyte ActivationMiceMice, KnockoutMice, TransgenicProtein SReceptor Protein-Tyrosine KinasesReverse Transcriptase Polymerase Chain ReactionSignal TransductionT-LymphocytesConceptsImmune responseDC activationProtein STAM receptor signalingDendritic cell activationExaggerated immune responseTAM receptor tyrosine kinasesDendritic cellsChronic inflammationCostimulatory moleculesImmune homeostasisAdaptive immunityCell activationInnate immunityGenetic ablationReceptor tyrosine kinasesReceptor signalingImmune defenseNegative feedback mechanismMouse TImmunityActivationTyrosine kinaseCellsPROS1
2011
T cell derived Protein S inhibits the activation of Dendritic cells through the TAM receptors Axl and Mer
Silva E, Chan P, Joannas L, Burstyn-Cohen T, Lemke G, Ghosh S, Rothlin C. T cell derived Protein S inhibits the activation of Dendritic cells through the TAM receptors Axl and Mer. Inflammatory Bowel Diseases 2011, 17: s10-s10. DOI: 10.1097/00054725-201112002-00028.Peer-Reviewed Original Research
2002
CK2 constitutively associates with and phosphorylates chicken erythroid ankyrin and regulates its ability to bind to spectrin
Ghosh S, Dorsey FC, Cox JV. CK2 constitutively associates with and phosphorylates chicken erythroid ankyrin and regulates its ability to bind to spectrin. Journal Of Cell Science 2002, 115: 4107-4115. PMID: 12356915, DOI: 10.1242/jcs.00102.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnkyrinsAntibody SpecificityCasein Kinase IICell MembraneChick EmbryoCytoskeletonDogsEnzyme InhibitorsEpithelial CellsErythroid Precursor CellsMacromolecular SubstancesPhosphoric Monoester HydrolasesPhosphorylationPhosphotransferasesPrecipitin TestsProtein BindingProtein IsoformsProtein Serine-Threonine KinasesSpectrinSubcellular FractionsConceptsErythroid ankyrinKidney epithelial cellsMadin-Darby canine kidney epithelial cellsCK2-specific inhibitorCanine kidney epithelial cellsEpithelial cellsPhosphorylation eventsPhosphatase inhibitorSpectrin cytoskeletonMembrane cytoskeletonPhosphorylation stateCK2Erythroid cellsAnkyrinCytoskeletonInhibitor studiesCell typesPhosphorylationSpectrinKinaseCellsFirst demonstrationVivoInhibitorsComplexes
2001
Dynamics of Ankyrin-containing Complexes in Chicken Embryonic Erythroid Cells: Role of Phosphorylation
Ghosh S, Cox J. Dynamics of Ankyrin-containing Complexes in Chicken Embryonic Erythroid Cells: Role of Phosphorylation. Molecular Biology Of The Cell 2001, 12: 3864-3874. PMID: 11739786, PMCID: PMC60761, DOI: 10.1091/mbc.12.12.3864.Peer-Reviewed Original ResearchConceptsCytoskeletal associationPhosphatase inhibitorErythroid ankyrinErythroid cellsEmbryonic erythroid cellsRole of phosphorylationAE1 anion exchangerTreatment of cellsBind spectrinAnkyrin isoformsAnkyrinCytoskeletal spectrinDynamic rearrangementPhosphorylationSoluble poolVivo consequencesSpectrinComplex formationSerineRapid cyclesIsoformsCellsPoolInhibitorsComplexes