Single-cell longitudinal analysis of SARS-CoV-2 infection in human airway epithelium identifies target cells, alterations in gene expression, and cell state changes
Ravindra NG, Alfajaro MM, Gasque V, Huston NC, Wan H, Szigeti-Buck K, Yasumoto Y, Greaney AM, Habet V, Chow RD, Chen JS, Wei J, Filler RB, Wang B, Wang G, Niklason LE, Montgomery RR, Eisenbarth SC, Chen S, Williams A, Iwasaki A, Horvath TL, Foxman EF, Pierce RW, Pyle AM, van Dijk D, Wilen CB. Single-cell longitudinal analysis of SARS-CoV-2 infection in human airway epithelium identifies target cells, alterations in gene expression, and cell state changes. PLOS Biology 2021, 19: e3001143. PMID: 33730024, PMCID: PMC8007021, DOI: 10.1371/journal.pbio.3001143.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectionSARS-CoV-2Human bronchial epithelial cellsInterferon-stimulated genesCell state changesAcute respiratory syndrome coronavirus 2 infectionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infectionSyndrome coronavirus 2 infectionCell tropismCoronavirus 2 infectionCoronavirus disease 2019Onset of infectionCell-intrinsic expressionCourse of infectionAir-liquid interface culturesHost-viral interactionsBronchial epithelial cellsSingle-cell RNA sequencingCell typesIL-1Disease 2019Human airwaysDevelopment of therapeuticsDrug AdministrationViral replicationThe aging transcriptome and cellular landscape of the human lung in relation to SARS-CoV-2
Chow RD, Majety M, Chen S. The aging transcriptome and cellular landscape of the human lung in relation to SARS-CoV-2. Nature Communications 2021, 12: 4. PMID: 33397975, PMCID: PMC7782551, DOI: 10.1038/s41467-020-20323-9.Peer-Reviewed Original ResearchConceptsSARS-CoV-2SARS-CoV-2 infectionHuman lungCOVID-19Natural killer/T-cellAirway smooth muscle cellsSevere coronavirus diseaseSevere COVID-19Alveolar type 2 cellsMajor risk factorType 2 cellsSmooth muscle cellsSARS-CoV-2 proteomeAge-associated genesAge-associated changesDendritic cellsRisk factorsT cellsGoblet cellsAlveolar fibroblastsCoronavirus diseaseMuscle cellsOlder populationCellular landscapeEndothelial cells