2018
Mapping a functional cancer genome atlas of tumor suppressors in mouse liver using AAV-CRISPR–mediated direct in vivo screening
Wang G, Chow RD, Ye L, Guzman CD, Dai X, Dong MB, Zhang F, Sharp PA, Platt RJ, Chen S. Mapping a functional cancer genome atlas of tumor suppressors in mouse liver using AAV-CRISPR–mediated direct in vivo screening. Science Advances 2018, 4: eaao5508. PMID: 29503867, PMCID: PMC5829971, DOI: 10.1126/sciadv.aao5508.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarcinogenesisClustered Regularly Interspaced Short Palindromic RepeatsDependovirusGenes, Tumor SuppressorGenetic TestingGenomeLiverMiceMutationNeoplasmsConceptsTumor suppressorCancer Genome AtlasHuman cancersSgRNA target sitesGenome AtlasCancer Genomics ConsortiumPutative tumor suppressor geneNumerous human cancersTumor suppressor geneCRISPR screensClassical oncogenesGenomics ConsortiumSuppressor geneFunctional variantsFunctional consequencesMutational landscapeAutochthonous mouse modelSuppressorTarget siteAAV-CRISPRGenesMouse liverMultiple variantsLiver tumorigenesisVivo
2014
CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling
Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F. CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell 2014, 159: 440-455. PMID: 25263330, PMCID: PMC4265475, DOI: 10.1016/j.cell.2014.09.014.Peer-Reviewed Original ResearchCRISPR-mediated direct mutation of cancer genes in the mouse liver
Xue W, Chen S, Yin H, Tammela T, Papagiannakopoulos T, Joshi NS, Cai W, Yang G, Bronson R, Crowley DG, Zhang F, Anderson DG, Sharp PA, Jacks T. CRISPR-mediated direct mutation of cancer genes in the mouse liver. Nature 2014, 514: 380-384. PMID: 25119044, PMCID: PMC4199937, DOI: 10.1038/nature13589.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBeta CateninCell Transformation, NeoplasticClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas SystemsFemaleGenes, p53Genes, Tumor SuppressorGenetic EngineeringHepatocytesLipid MetabolismLiverLiver NeoplasmsMiceMolecular Sequence DataMutagenesisMutationOncogenesPhosphorylationProto-Oncogene Proteins c-aktPTEN Phosphohydrolase