Programmable DNA repair with CRISPRa/i enhanced homology-directed repair efficiency with a single Cas9
Ye L, Wang C, Hong L, Sun N, Chen D, Chen S, Han F. Programmable DNA repair with CRISPRa/i enhanced homology-directed repair efficiency with a single Cas9. Cell Discovery 2018, 4: 46. PMID: 30062046, PMCID: PMC6056518, DOI: 10.1038/s41421-018-0049-7.Peer-Reviewed Original ResearchHomology-directed repairDNA double-strand breaksCRISPRa/iGene editingDNA repairHDR efficiencySite-specific genome engineeringPrecise genome modificationNon-homologous DNA endDNA repair factorsRNA interference-mediated inhibitionDNA repair pathwaysDouble-strand breaksGene editing processHomology-directed repair efficiencyPrecise gene editingGenome modificationGenome engineeringRepair factorsMammalian cellsHDR pathwayNHEJ factorsDNA endsGuide RNAHDR rates