2017
Circular oligomerization is an intrinsic property of synaptotagmin
Wang J, Li F, Bello OD, Sindelar CV, Pincet F, Krishnakumar SS, Rothman JE. Circular oligomerization is an intrinsic property of synaptotagmin. ELife 2017, 6: e27441. PMID: 28850328, PMCID: PMC5576491, DOI: 10.7554/elife.27441.Peer-Reviewed Original Research
2013
Conformational Dynamics of Calcium-Triggered Activation of Fusion by Synaptotagmin
Krishnakumar SS, Kümmel D, Jones SJ, Radoff DT, Reinisch KM, Rothman JE. Conformational Dynamics of Calcium-Triggered Activation of Fusion by Synaptotagmin. Biophysical Journal 2013, 105: 2507-2516. PMID: 24314081, PMCID: PMC3853086, DOI: 10.1016/j.bpj.2013.10.029.Peer-Reviewed Original Research
2011
A conformational switch in complexin is required for synaptotagmin to trigger synaptic fusion
Krishnakumar SS, Radoff DT, Kümmel D, Giraudo CG, Li F, Khandan L, Baguley SW, Coleman J, Reinisch KM, Pincet F, Rothman JE. A conformational switch in complexin is required for synaptotagmin to trigger synaptic fusion. Nature Structural & Molecular Biology 2011, 18: 934-940. PMID: 21785412, PMCID: PMC3668341, DOI: 10.1038/nsmb.2103.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Vesicular TransportAmino Acid SequenceAnimalsBinding SitesCrystallography, X-RayHumansMembrane FusionModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedNerve Tissue ProteinsProtein Structure, TertiaryRatsSynaptosomal-Associated Protein 25SynaptotagminsSyntaxin 1Vesicle-Associated Membrane Protein 2Complexin cross-links prefusion SNAREs into a zigzag array
Kümmel D, Krishnakumar SS, Radoff DT, Li F, Giraudo CG, Pincet F, Rothman JE, Reinisch KM. Complexin cross-links prefusion SNAREs into a zigzag array. Nature Structural & Molecular Biology 2011, 18: 927-933. PMID: 21785414, PMCID: PMC3410656, DOI: 10.1038/nsmb.2101.Peer-Reviewed Original Research
2009
The Effect of Hydrophilic Substitutions and Anionic Lipids upon the Transverse Positioning of the Transmembrane Helix of the ErbB2 (neu) Protein Incorporated into Model Membrane Vesicles
Shahidullah K, Krishnakumar SS, London E. The Effect of Hydrophilic Substitutions and Anionic Lipids upon the Transverse Positioning of the Transmembrane Helix of the ErbB2 (neu) Protein Incorporated into Model Membrane Vesicles. Journal Of Molecular Biology 2009, 396: 209-220. PMID: 19931543, PMCID: PMC2821092, DOI: 10.1016/j.jmb.2009.11.037.Peer-Reviewed Original Research
2007
The Control of Transmembrane Helix Transverse Position in Membranes by Hydrophilic Residues
Krishnakumar SS, London E. The Control of Transmembrane Helix Transverse Position in Membranes by Hydrophilic Residues. Journal Of Molecular Biology 2007, 374: 1251-1269. PMID: 17997412, PMCID: PMC2175128, DOI: 10.1016/j.jmb.2007.10.032.Peer-Reviewed Original ResearchMembrane Topography of the Hydrophobic Anchor Sequence of Poliovirus 3A and 3AB Proteins and the Functional Effect of 3A/3AB Membrane Association upon RNA Replication †
Fujita K, Krishnakumar SS, Franco D, Paul AV, London E, Wimmer E. Membrane Topography of the Hydrophobic Anchor Sequence of Poliovirus 3A and 3AB Proteins and the Functional Effect of 3A/3AB Membrane Association upon RNA Replication †. Biochemistry 2007, 46: 5185-5199. PMID: 17417822, PMCID: PMC2519882, DOI: 10.1021/bi6024758.Peer-Reviewed Original ResearchConceptsModel membrane vesiclesAnchor sequenceHydrophobic anchor sequenceMembrane vesiclesViral protein 3ABFull-length proteinN-terminal boundaryTransmembrane topographyMembrane associationSuppressor mutationsTransmembrane segmentsProtein 3ABRNA polymeraseMembranous vesiclesC-terminusMembrane topographyCytoplasmic surfaceHost cellsRNA replicationPoliovirus RNAHeLa cellsSingle tryptophanProteinUridylylation reactionLipid bilayers
2003
Active and Inactive Orientations of the Transmembrane and Cytosolic Domains of the Erythropoietin Receptor Dimer
Seubert N, Royer Y, Staerk J, Kubatzky KF, Moucadel V, Krishnakumar S, Smith SO, Constantinescu SN. Active and Inactive Orientations of the Transmembrane and Cytosolic Domains of the Erythropoietin Receptor Dimer. Molecular Cell 2003, 12: 1239-1250. PMID: 14636581, DOI: 10.1016/s1097-2765(03)00389-7.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell LineDimerizationDNA-Binding ProteinsEnzyme ActivationErythroid Precursor CellsErythropoietinJanus Kinase 2MiceMilk ProteinsModels, MolecularMolecular Sequence DataProtein Structure, QuaternaryProtein Structure, SecondaryProtein Structure, TertiaryProtein-Tyrosine KinasesProto-Oncogene ProteinsReceptors, ErythropoietinRecombinant Fusion ProteinsSaccharomyces cerevisiae ProteinsSequence AlignmentSignal TransductionSTAT3 Transcription FactorSTAT5 Transcription FactorTrans-ActivatorsTranscription FactorsTranscription, GeneticViral Envelope Proteins