Quantitation of the ras gene product in leukemic blast cells using enzymatic staining.
Gutheil J, Mane S, Bass K, Lee E, Needleman S. Quantitation of the ras gene product in leukemic blast cells using enzymatic staining. BioTechniques 1990, 9: 212-7. PMID: 2205250.Peer-Reviewed Original ResearchConceptsRas gene productGene productsRas speciesRas protein levelsProtein of interestGene product levelsQuantitation of proteinsSpecific ras mutationsUse of electrophoresisProtein levelsRas mutationsProteinP21 expressionHuman malignanciesSpeciesCellsLeukemic cellsMutationsEnzymatic stainingHigh specificityRadioactive reagentsExpressionElectrophoresisAssaysVivoRAS gene activation in acute myelogenous leukemia: Analysis by in vitro amplification and dna base sequence determination
Mane S, Meltzer S, Gutheil J, Kapil V, Lee E, Needleman S. RAS gene activation in acute myelogenous leukemia: Analysis by in vitro amplification and dna base sequence determination. Genes Chromosomes And Cancer 1990, 2: 71-77. PMID: 2278967, DOI: 10.1002/gcc.2870020113.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCohort StudiesDNA Mutational AnalysisDNA, NeoplasmExonsGene Expression Regulation, NeoplasticGenes, rasHumansIatrogenic DiseaseLeukemia, Myeloid, AcuteMolecular Sequence DataPolymerase Chain ReactionProspective StudiesProto-Oncogene Proteins p21(ras)Transcriptional ActivationConceptsAcute myeloid leukemia patientsMaryland Cancer CenterMyeloid leukemia patientsAcute myelogenous leukemiaRAS gene activationProspective cohortCancer CenterLeukemia patientsPrecise prevalenceMyelogenous leukemiaNRAS mutationsRAS activationRAS mutationsGene point mutationsBiologic parametersLarger studyHuman cancersPatientsAMLProtooncogene activationExon mutationsActivationCell DNAMutationsPoint mutationsRAS gene product expression in blood and marrow smears of patients with acute leukemia: Importance of fixation
Needleman S, Cimino E, Mane S, Gutheil J, Kapil V, Chana G. RAS gene product expression in blood and marrow smears of patients with acute leukemia: Importance of fixation. Pathology 1990, 22: 77-81. PMID: 2235101, DOI: 10.3109/00313029009063784.Peer-Reviewed Original ResearchMeSH KeywordsBone MarrowFixativesHumansImmunoenzyme TechniquesLeukemia, Myeloid, AcuteProto-Oncogene Proteins p21(ras)ConceptsAcute non-lymphoblastic leukemiaP21 expressionAcute leukemiaMarrow aspiratesNon-lymphoblastic leukemiaNon-malignant diseasesHigh p21 expressionRas p21 expressionHistologic anaplasiaMost patientsRegional lymphLeukemic blastsProstate cancerBreast cancerGene product expressionPlasma cellsMarrow smearsNormal volunteersBlast cellsPatientsMyeloid cellsCodon 12Importance of fixationBlood smearsHuman malignancies