2003
Constitutive expression of cytotoxic proteases and down-regulation of protease inhibitors in LGL leukemia.
Kothapalli R, Bailey R, Kusmartseva I, Mane S, Epling-Burnette P, Loughran T. Constitutive expression of cytotoxic proteases and down-regulation of protease inhibitors in LGL leukemia. International Journal Of Oncology 2003, 22: 33-9. PMID: 12469182, DOI: 10.3892/ijo.22.1.33.Peer-Reviewed Original ResearchConceptsRNase protection assaysGene expressionPeripheral blood mononuclear cellsGroup of genesDifferential gene expressionNormal peripheral blood mononuclear cellsBlood mononuclear cellsNorthern blot analysisLGL leukemiaSmall subunitCaspase-8Protein perforinConstitutive expressionMicroarray technologyMononuclear cellsCytotoxic proteasesGenesProtection assaysCysteine proteinasesLarge granular lymphocyte leukemiaBlot analysisLGL leukemia patientsGranular lymphocyte leukemiaAlpha-1 antitrypsinExpression
1990
Quantitation of the ras gene product in leukemic blast cells using enzymatic staining.
Gutheil J, Mane S, Bass K, Lee E, Needleman S. Quantitation of the ras gene product in leukemic blast cells using enzymatic staining. BioTechniques 1990, 9: 212-7. PMID: 2205250.Peer-Reviewed Original ResearchConceptsRas gene productGene productsRas speciesRas protein levelsProtein of interestGene product levelsQuantitation of proteinsSpecific ras mutationsUse of electrophoresisProtein levelsRas mutationsProteinP21 expressionHuman malignanciesSpeciesCellsLeukemic cellsMutationsEnzymatic stainingHigh specificityRadioactive reagentsExpressionElectrophoresisAssaysVivo