2020
Designed Parasite-Selective Rhomboid Inhibitors Block Invasion and Clear Blood-Stage Malaria
Gandhi S, Baker R, Cho S, Stanchev S, Strisovsky K, Urban S. Designed Parasite-Selective Rhomboid Inhibitors Block Invasion and Clear Blood-Stage Malaria. Cell Chemical Biology 2020, 27: 1410-1424.e6. PMID: 32888502, PMCID: PMC7680425, DOI: 10.1016/j.chembiol.2020.08.011.Peer-Reviewed Original ResearchConceptsRhomboid proteolysisRhomboid intramembrane proteasesAtypical substrate specificityHost cell invasionHigh-resolution structuresRhomboid proteasesIntramembrane proteasesRhomboid inhibitorsSubstrate specificityBlocks invasionRapid invasionDisease contextsSteric clashesProteolysisUltrastructural analysisProteaseInvasionPathophysiological processesPeptide boronatesBlood-stage malariaSteric exclusionParasitesMalaria culturesSequenceTargeting
2015
A Trichomonas vaginalis Rhomboid Protease and Its Substrate Modulate Parasite Attachment and Cytolysis of Host Cells
Riestra A, Gandhi S, Sweredoski M, Moradian A, Hess S, Urban S, Johnson P. A Trichomonas vaginalis Rhomboid Protease and Its Substrate Modulate Parasite Attachment and Cytolysis of Host Cells. PLOS Pathogens 2015, 11: e1005294. PMID: 26684303, PMCID: PMC4684317, DOI: 10.1371/journal.ppat.1005294.Peer-Reviewed Original ResearchConceptsT. vaginalis pathogenesisRhomboid proteasesHost cellsCell surface membrane proteinsIntramembrane serine proteasesDifferent subcellular localizationsSurface membrane proteinsAmino acid residuesSmall amino acidsEukaryotic parasitesTransmembrane domainBioinformatics approachMembrane proteinsSubcellular localizationSurface proteomeSubstrate specificityMolecular mechanismsGolgi apparatusAcid residuesProtease substratesCleavage assaysCleavage siteAmino acidsCell surfaceParasite attachment