2016
C9orf72 binds SMCR8, localizes to lysosomes, and regulates mTORC1 signaling
Amick J, Roczniak-Ferguson A, Ferguson SM. C9orf72 binds SMCR8, localizes to lysosomes, and regulates mTORC1 signaling. Molecular Biology Of The Cell 2016, 27: 3040-3051. PMID: 27559131, PMCID: PMC5063613, DOI: 10.1091/mbc.e16-01-0003.Peer-Reviewed Original ResearchConceptsAmino acid availabilityAcid availabilityGenome-editing strategiesKO cell linesProtein complexesSubcellular localizationKnockout phenotypesC9orf72 proteinLysosomal siteBioinformatics predictionSMCR8Tumor suppressorSwollen lysosomesFunctional interactionLysosomesC9orf72 geneCell linesStructural similarityNormal functionC9orf72PhenotypeAmyotrophic lateral sclerosisBirt-HoggIntronsMTORC1
2013
Recruitment of folliculin to lysosomes supports the amino acid–dependent activation of Rag GTPases
Petit CS, Roczniak-Ferguson A, Ferguson SM. Recruitment of folliculin to lysosomes supports the amino acid–dependent activation of Rag GTPases. Journal Of Cell Biology 2013, 202: 1107-1122. PMID: 24081491, PMCID: PMC3787382, DOI: 10.1083/jcb.201307084.Peer-Reviewed Original ResearchMeSH KeywordsAmino AcidsBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsBlotting, WesternCarrier ProteinsCytoplasmFluorescent Antibody TechniqueHumansImmunoprecipitationLysosomesMechanistic Target of Rapamycin Complex 1Monomeric GTP-Binding ProteinsMultiprotein ComplexesProto-Oncogene ProteinsRecombinant ProteinsRNA, Small InterferingTOR Serine-Threonine KinasesTumor Suppressor ProteinsConceptsAmino acid-dependent activationAcid-dependent activationTranscription factor EBRag GTPasesSurface of lysosomesMTORC1-dependent phosphorylationAmino acid depletionLysosome recruitmentGTPase domainRAG interactionsCytoplasmic sequestrationLysosome functionGTPasesFLCNHuman diseasesFunction mutationsDevelopment of pneumothoraxProtein 1Direct interactionLysosomesCritical rolePulmonary cystsSite of actionAcid depletionFolliculin gene
2000
Molecular Cloning of a Human, Hemicholinium-3-Sensitive Choline Transporter
Apparsundaram S, Ferguson S, George A, Blakely R. Molecular Cloning of a Human, Hemicholinium-3-Sensitive Choline Transporter. Biochemical And Biophysical Research Communications 2000, 276: 862-867. PMID: 11027560, DOI: 10.1006/bbrc.2000.3561.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBinding SitesBiological TransportCarrier ProteinsCholineChromosomes, Human, Pair 2Cloning, MolecularCOS CellsHemicholinium 3HumansLigandsMembrane Transport ProteinsMolecular Sequence DataOocytesProtein BindingRadiation Hybrid MappingRNA, MessengerSequence Homology, Amino AcidTransfectionXenopus laevisConceptsRadiation hybrid mapping techniquesCOS-7 cells resultsTransporter gene familyGlucose transporter gene familyHuman Genome ProjectNorthern blot analysisChromosomal localizationGene familyMolecular cloningGenome ProjectKb transcriptSequence informationMembrane vesiclesPrimary structureDependent choline uptakeMembrane fractionAmino acidsCDNAIodide transporterCells resultsBlot analysisChromosome 2q12Biosynthesis of acetylcholineMechanistic analysisCHO-1