2019
Weak membrane interactions allow Rheb to activate mTORC1 signaling without major lysosome enrichment
Angarola B, Ferguson SM. Weak membrane interactions allow Rheb to activate mTORC1 signaling without major lysosome enrichment. Molecular Biology Of The Cell 2019, 30: 2750-2760. PMID: 31532697, PMCID: PMC6789162, DOI: 10.1091/mbc.e19-03-0146.Peer-Reviewed Original ResearchMeSH KeywordsAmino AcidsAnimalsChlorocebus aethiopsCOS CellsEndoplasmic ReticulumHeLa CellsHumansLysosomesMechanistic Target of Rapamycin Complex 1Monomeric GTP-Binding ProteinsMultiprotein ComplexesNeuropeptidesPrenylationRas Homolog Enriched in Brain ProteinSignal TransductionTOR Serine-Threonine KinasesConceptsMembrane interactionsC-terminal CAAX motifTransient membrane interactionsEndoplasmic reticulum localizationMTOR complex 1CAAX motifRheb GTPaseER membraneMTORC1 activationSubcellular localizationTargeting motifRhebLysosome enrichmentHuman cellsFunctional assaysTargeting mechanismStable interactionStable localizationLysosomesFurther systematic analysisMotifActivation
2000
Molecular Cloning of a Human, Hemicholinium-3-Sensitive Choline Transporter
Apparsundaram S, Ferguson S, George A, Blakely R. Molecular Cloning of a Human, Hemicholinium-3-Sensitive Choline Transporter. Biochemical And Biophysical Research Communications 2000, 276: 862-867. PMID: 11027560, DOI: 10.1006/bbrc.2000.3561.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBinding SitesBiological TransportCarrier ProteinsCholineChromosomes, Human, Pair 2Cloning, MolecularCOS CellsHemicholinium 3HumansLigandsMembrane Transport ProteinsMolecular Sequence DataOocytesProtein BindingRadiation Hybrid MappingRNA, MessengerSequence Homology, Amino AcidTransfectionXenopus laevisConceptsRadiation hybrid mapping techniquesCOS-7 cells resultsTransporter gene familyGlucose transporter gene familyHuman Genome ProjectNorthern blot analysisChromosomal localizationGene familyMolecular cloningGenome ProjectKb transcriptSequence informationMembrane vesiclesPrimary structureDependent choline uptakeMembrane fractionAmino acidsCDNAIodide transporterCells resultsBlot analysisChromosome 2q12Biosynthesis of acetylcholineMechanistic analysisCHO-1