Novel Fluorescent Timer Tool Enables Characterization of Erythropoietic Differentiation Based on Differential Cell Cycling Speeds
Modepalli S, Eastman A, Shaw C, Guo S, Hattangadi S, Kupfer G. Novel Fluorescent Timer Tool Enables Characterization of Erythropoietic Differentiation Based on Differential Cell Cycling Speeds. Blood 2020, 136: 27-28. DOI: 10.1182/blood-2020-141666.Peer-Reviewed Original ResearchCell cycle speedCell divisionErythroid differentiationFusion proteinOrthochromatic erythroblastsLive-cell reporterStress erythropoiesisFluorescent timer proteinsAvailable proteomic dataSubsequent cell divisionRapid cell divisionUpregulation of genesCell cycle dynamicsErythroid cell proliferationSingle-cell levelRed fluorescent proteinFaster cycling cellsCell cycle behaviorFT proteinPhosphoproteomic investigationsDiscrete molecular targetsInducible promoterProteomic dataActive lociStress hematopoiesis2014 – FLUORESCENT CELL CYCLE TIMER ENABLED ANALYSIS OF NORMAL AND INEFFECTIVE ERYTHROPOIESIS
Modepalli S, Eastman A, Shaw C, Guo S, Hattangadi S, Kupfer G. 2014 – FLUORESCENT CELL CYCLE TIMER ENABLED ANALYSIS OF NORMAL AND INEFFECTIVE ERYTHROPOIESIS. Experimental Hematology 2020, 88: s32. DOI: 10.1016/j.exphem.2020.09.176.Peer-Reviewed Original ResearchCell cycle speedCell cycleLive-cell reporterFluorescent timer proteinsErythropoietic responseIneffective erythropoiesisFaster cycling cellsErythroblast stageFlow cytometric sortingStress hematopoiesisDiamond-Blackfan anemiaTimer proteinFusion proteinCellular factorsEarly progenitorsCell stagePrimary cell culturesSorted populationsCell cycling ratesMetabolic pathwaysCycling cellsStress erythropoiesisHuman CD34Cytometric sortingIntracellular ratioResolving Cell Cycle Speed in One Snapshot with a Live-Cell Fluorescent Reporter
Eastman AE, Chen X, Hu X, Hartman AA, Morales A, Yang C, Lu J, Kueh HY, Guo S. Resolving Cell Cycle Speed in One Snapshot with a Live-Cell Fluorescent Reporter. Cell Reports 2020, 31: 107804. PMID: 32579930, PMCID: PMC7418154, DOI: 10.1016/j.celrep.2020.107804.Peer-Reviewed Original ResearchConceptsFluorescent reportersLive-cell fluorescent reporterCell cycle speedFluorescent timer proteinsCell proliferationCell cycle dynamicsRed fluorescent proteinFaster cycling cellsFate transitionsFusion reporterActive lociTimer proteinFluorescent proteinLength heterogeneityComplex tissuesHematopoietic cellsCycling cellsReporterFluorescence ratioCycle dynamicsProteinFunctional heterogeneityMouse strainsSolid tissuesCycle speed